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NO mediated increase of Fos protein and NMDA1A R mRNA expression in rat spinal cord during morphine withdrawal.
Acta Pharmacol Sin. 2001 Jun; 22(6):505-11.AP

Abstract

AIM

To investigate the effects of nitric oxide (NO) on activation of the rat spinal cord neurons during naloxone-precipitated morphine withdrawal.

METHODS

Fos immunocytochemistry, NADPH-d histochemistry, Fos/NADPH-d double-labeling, intrathecal injection, antisense oligonucleotides (AS-ONs) techniques, and RT-PCR were used.

RESULTS

Acute administration of naloxone and chronic administration of morphine did not change the expression of Fos protein and NADPH-d positive neurons, and there was no expression of Fos/NADPH-d double-labeled neurons in the spinal cord of rats. Morphine withdrawal increased the expression of Fos protein, NADPH-d positive, and Fos/NADPH-d double-labeled neurons, and they were observed in all the laminae of the rat spinal cord. Intrathecal injection of nNOS antisense oligonucleotides (nNOS-AS) inhibited the increase of Fos protein and NMDA(1A)R mRNA expression in the rat spinal cord during morphine withdrawal and decreased the scores of morphine withdrawal symptoms. The effect of nNOS-AS was greater than that of eNOS-AS. There was no effect in nNOS sense oligonucleotides (nNOS-S) group.

CONCLUSION

NO mediated the increase of Fos protein and NMDA1A R mRNA expression in the rat spinal cord during morphine withdrawal.

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Authors+Show Affiliations

Gao JL
Department of Anesthesiology, Affiliated Hospital of Xuzhou Medical College, Xuzhou 221002, China.
Zeng YM
No affiliation info available
Zhang LC
No affiliation info available
Gu J
No affiliation info available
Liu HF
No affiliation info available
Zhou WH
No affiliation info available
Yang GD
No affiliation info available

MeSH

AnimalsMaleMorphineNitric OxideNitric Oxide SynthaseNitric Oxide Synthase Type IOligonucleotides, AntisenseProto-Oncogene Proteins c-fosRNA, MessengerRatsRats, Sprague-DawleyReceptors, N-Methyl-D-AspartateSpinal CordSubstance Withdrawal Syndrome

Pub Type(s)

Journal Article

Language

eng

PubMed ID

11747755

Citation

Gao, J L., et al. "NO Mediated Increase of Fos Protein and NMDA1A R mRNA Expression in Rat Spinal Cord During Morphine Withdrawal." Acta Pharmacologica Sinica, vol. 22, no. 6, 2001, pp. 505-11.
Gao JL, Zeng YM, Zhang LC, et al. NO mediated increase of Fos protein and NMDA1A R mRNA expression in rat spinal cord during morphine withdrawal. Acta Pharmacol Sin. 2001;22(6):505-11.
Gao, J. L., Zeng, Y. M., Zhang, L. C., Gu, J., Liu, H. F., Zhou, W. H., & Yang, G. D. (2001). NO mediated increase of Fos protein and NMDA1A R mRNA expression in rat spinal cord during morphine withdrawal. Acta Pharmacologica Sinica, 22(6), 505-11.
Gao JL, et al. NO Mediated Increase of Fos Protein and NMDA1A R mRNA Expression in Rat Spinal Cord During Morphine Withdrawal. Acta Pharmacol Sin. 2001;22(6):505-11. PubMed PMID: 11747755.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - NO mediated increase of Fos protein and NMDA1A R mRNA expression in rat spinal cord during morphine withdrawal. AU - Gao,J L, AU - Zeng,Y M, AU - Zhang,L C, AU - Gu,J, AU - Liu,H F, AU - Zhou,W H, AU - Yang,G D, PY - 2001/12/19/pubmed PY - 2003/6/19/medline PY - 2001/12/19/entrez SP - 505 EP - 11 JF - Acta pharmacologica Sinica JO - Acta Pharmacol Sin VL - 22 IS - 6 N2 - AIM: To investigate the effects of nitric oxide (NO) on activation of the rat spinal cord neurons during naloxone-precipitated morphine withdrawal. METHODS: Fos immunocytochemistry, NADPH-d histochemistry, Fos/NADPH-d double-labeling, intrathecal injection, antisense oligonucleotides (AS-ONs) techniques, and RT-PCR were used. RESULTS: Acute administration of naloxone and chronic administration of morphine did not change the expression of Fos protein and NADPH-d positive neurons, and there was no expression of Fos/NADPH-d double-labeled neurons in the spinal cord of rats. Morphine withdrawal increased the expression of Fos protein, NADPH-d positive, and Fos/NADPH-d double-labeled neurons, and they were observed in all the laminae of the rat spinal cord. Intrathecal injection of nNOS antisense oligonucleotides (nNOS-AS) inhibited the increase of Fos protein and NMDA(1A)R mRNA expression in the rat spinal cord during morphine withdrawal and decreased the scores of morphine withdrawal symptoms. The effect of nNOS-AS was greater than that of eNOS-AS. There was no effect in nNOS sense oligonucleotides (nNOS-S) group. CONCLUSION: NO mediated the increase of Fos protein and NMDA1A R mRNA expression in the rat spinal cord during morphine withdrawal. SN - 1671-4083 UR - https://www.unboundmedicine.com/medline/citation/11747755/NO_mediated_increase_of_Fos_protein_and_NMDA1A_R_mRNA_expression_in_rat_spinal_cord_during_morphine_withdrawal_ L2 - http://www.chinaphar.com/1671-4083/22/505.pdf DB - PRIME DP - Unbound Medicine ER -