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Antitissue transglutaminase antibodies outside celiac disease.
J Pediatr Gastroenterol Nutr 2002; 34(1):31-4JP

Abstract

BACKGROUND

Tissue transglutaminase enzyme-linked immunosorbent assay (tTG-ELISA) has recently been proposed as a simple and fast screening test for celiac disease (CD). The rate of false-positive and false-negative tests with tTG-ELISA, however, has not been definitively established. Therefore, the aim of our study was to investigate anti-tTG antibodies (TGA) not only in untreated patients with CD and in healthy controls, but also in a large group of patients with other autoimmune diseases.

METHODS

The presence of TGA was investigated in sera from 111 patients with untreated CD, 96 patients with other autoimmune conditions (28 with autoimmune liver disease, 46 with insulin-dependent diabetes mellitus, 10 with inflammatory bowel syndrome, 12 with type 1 polyglandular syndrome) and from 100 healthy controls using guinea pig tTG-ELISA (gp-TG/ELISA) and highly purified recombinant human tTG-ELISA (h-TG/ELISA). Western blotting with guinea pig tTG was also performed.

RESULTS

Ninety-four patients with CD who tested positive for antiendomysial antibodies (AEA) and one who tested negative for AEA showed antibodies against the gp-TG. Among the controls, 50% of patients with autoimmune liver disease and 6.5% of patients with insulin-dependent diabetes mellitus tested positive with gp-TG/ELISA. Western blotting experiments revealed that the high rate of positive tests observed using ELISA among the control group sera is attributable to impurities in the gp-TG preparation. However, h-TG/ELISA tests were positive for the sera from all patients who tested positive for AEA and from one control who tested negative for AEA, whereas h-TG/ELISA tests were negative for all CD patients who tested negative for AEA and for other controls who tested negative for AEA.

CONCLUSIONS

The frequency of false-negative and false-positive tests represents the major limit to the use of gp-tTG/ELISA. However, because h-TG/ELISA is both simple and fast, it could be used in large screening programs for CD.

Authors+Show Affiliations

Department of Biomedical Sciences and Biotechnologies, Second Pediatric Clinic, Cagliari University, Cagliari, Italy.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

11753161

Citation

Clemente, Maria Grazia, et al. "Antitissue Transglutaminase Antibodies Outside Celiac Disease." Journal of Pediatric Gastroenterology and Nutrition, vol. 34, no. 1, 2002, pp. 31-4.
Clemente MG, Musu MP, Frau F, et al. Antitissue transglutaminase antibodies outside celiac disease. J Pediatr Gastroenterol Nutr. 2002;34(1):31-4.
Clemente, M. G., Musu, M. P., Frau, F., Lucia, C., & De Virgiliis, S. (2002). Antitissue transglutaminase antibodies outside celiac disease. Journal of Pediatric Gastroenterology and Nutrition, 34(1), pp. 31-4.
Clemente MG, et al. Antitissue Transglutaminase Antibodies Outside Celiac Disease. J Pediatr Gastroenterol Nutr. 2002;34(1):31-4. PubMed PMID: 11753161.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Antitissue transglutaminase antibodies outside celiac disease. AU - Clemente,Maria Grazia, AU - Musu,Maria Paola, AU - Frau,Fulvia, AU - Lucia,Cicotto, AU - De Virgiliis,Stefano, PY - 2001/12/26/pubmed PY - 2002/5/3/medline PY - 2001/12/26/entrez SP - 31 EP - 4 JF - Journal of pediatric gastroenterology and nutrition JO - J. Pediatr. Gastroenterol. Nutr. VL - 34 IS - 1 N2 - BACKGROUND: Tissue transglutaminase enzyme-linked immunosorbent assay (tTG-ELISA) has recently been proposed as a simple and fast screening test for celiac disease (CD). The rate of false-positive and false-negative tests with tTG-ELISA, however, has not been definitively established. Therefore, the aim of our study was to investigate anti-tTG antibodies (TGA) not only in untreated patients with CD and in healthy controls, but also in a large group of patients with other autoimmune diseases. METHODS: The presence of TGA was investigated in sera from 111 patients with untreated CD, 96 patients with other autoimmune conditions (28 with autoimmune liver disease, 46 with insulin-dependent diabetes mellitus, 10 with inflammatory bowel syndrome, 12 with type 1 polyglandular syndrome) and from 100 healthy controls using guinea pig tTG-ELISA (gp-TG/ELISA) and highly purified recombinant human tTG-ELISA (h-TG/ELISA). Western blotting with guinea pig tTG was also performed. RESULTS: Ninety-four patients with CD who tested positive for antiendomysial antibodies (AEA) and one who tested negative for AEA showed antibodies against the gp-TG. Among the controls, 50% of patients with autoimmune liver disease and 6.5% of patients with insulin-dependent diabetes mellitus tested positive with gp-TG/ELISA. Western blotting experiments revealed that the high rate of positive tests observed using ELISA among the control group sera is attributable to impurities in the gp-TG preparation. However, h-TG/ELISA tests were positive for the sera from all patients who tested positive for AEA and from one control who tested negative for AEA, whereas h-TG/ELISA tests were negative for all CD patients who tested negative for AEA and for other controls who tested negative for AEA. CONCLUSIONS: The frequency of false-negative and false-positive tests represents the major limit to the use of gp-tTG/ELISA. However, because h-TG/ELISA is both simple and fast, it could be used in large screening programs for CD. SN - 0277-2116 UR - https://www.unboundmedicine.com/medline/citation/11753161/Antitissue_transglutaminase_antibodies_outside_celiac_disease_ L2 - http://dx.doi.org/10.1097/00005176-200201000-00008 DB - PRIME DP - Unbound Medicine ER -