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Adrenomedullin inhibits neurotransmission of calcitonin gene-related peptide (CGRP)-containing vasodilator nerves in rat mesenteric resistance arteries.
Peptides. 2001 Nov; 22(11):1887-93.P

Abstract

We have reported that the rat mesenteric resistance artery has innervation of calcitonin gene-related peptide (CGRP)-containing vasodilator nerves (CGRPergic nerves). We also demonstrated that adrenomedullin (AM) causes mesenteric vasodilation through activation of CGRP receptors. The present study was designed to examine the effect of AM on neurotransmission of CGRPergic nerves in rat mesenteric arteries. In preconstricted preparations without endothelium, periarterial nerve stimulation (PNS, 1 and 2 Hz) induced a frequency-dependent vasodilation. A bolus injection of CGRP (10 pmol) into the perfusate also caused a vasodilation. AM (0.1 to 10 nM) concentration-dependently caused 40% to 60% inhibition of the PNS-induced vasodilation, but AM did not attenuate vasodilation induced by exogenous CGRP injection. The inhibitory effect of AM (10 nM) on PNS-induced vasodilation was further potentiated by CGRP [8-37] (CGRP receptor antagonist, 50 nM), which attenuated the vasodilator response to the CGRP injection. Combined perfusion of AM [22-52] (AM receptor antagonist, 10 to 100 nM) resulted in further inhibition of PNS-induced neurogenic vasodilation without affecting the vasodilator response to the CGRP injection. CGRP [8-37] but not AM [22-52] antagonized vasodilation induced by AM perfusion. These findings suggest that AM presynaptically inhibits neurotransmission of CGRPergic nerves, probably decreasing CGRP release, via receptors different from CGRP receptors.

Authors+Show Affiliations

Department of Clinical Pharmaceutical Science, Graduate School of Natural Science and Technology, Okayama University, 1-1-1 Tsushima-naka, 700-8530, Okayama, Japan.No affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

11754977

Citation

Akiyama, S, et al. "Adrenomedullin Inhibits Neurotransmission of Calcitonin Gene-related Peptide (CGRP)-containing Vasodilator Nerves in Rat Mesenteric Resistance Arteries." Peptides, vol. 22, no. 11, 2001, pp. 1887-93.
Akiyama S, Kawasaki H, Shimogai A, et al. Adrenomedullin inhibits neurotransmission of calcitonin gene-related peptide (CGRP)-containing vasodilator nerves in rat mesenteric resistance arteries. Peptides. 2001;22(11):1887-93.
Akiyama, S., Kawasaki, H., Shimogai, A., & Kurosaki, Y. (2001). Adrenomedullin inhibits neurotransmission of calcitonin gene-related peptide (CGRP)-containing vasodilator nerves in rat mesenteric resistance arteries. Peptides, 22(11), 1887-93.
Akiyama S, et al. Adrenomedullin Inhibits Neurotransmission of Calcitonin Gene-related Peptide (CGRP)-containing Vasodilator Nerves in Rat Mesenteric Resistance Arteries. Peptides. 2001;22(11):1887-93. PubMed PMID: 11754977.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Adrenomedullin inhibits neurotransmission of calcitonin gene-related peptide (CGRP)-containing vasodilator nerves in rat mesenteric resistance arteries. AU - Akiyama,S, AU - Kawasaki,H, AU - Shimogai,A, AU - Kurosaki,Y, PY - 2002/1/5/pubmed PY - 2002/2/28/medline PY - 2002/1/5/entrez SP - 1887 EP - 93 JF - Peptides JO - Peptides VL - 22 IS - 11 N2 - We have reported that the rat mesenteric resistance artery has innervation of calcitonin gene-related peptide (CGRP)-containing vasodilator nerves (CGRPergic nerves). We also demonstrated that adrenomedullin (AM) causes mesenteric vasodilation through activation of CGRP receptors. The present study was designed to examine the effect of AM on neurotransmission of CGRPergic nerves in rat mesenteric arteries. In preconstricted preparations without endothelium, periarterial nerve stimulation (PNS, 1 and 2 Hz) induced a frequency-dependent vasodilation. A bolus injection of CGRP (10 pmol) into the perfusate also caused a vasodilation. AM (0.1 to 10 nM) concentration-dependently caused 40% to 60% inhibition of the PNS-induced vasodilation, but AM did not attenuate vasodilation induced by exogenous CGRP injection. The inhibitory effect of AM (10 nM) on PNS-induced vasodilation was further potentiated by CGRP [8-37] (CGRP receptor antagonist, 50 nM), which attenuated the vasodilator response to the CGRP injection. Combined perfusion of AM [22-52] (AM receptor antagonist, 10 to 100 nM) resulted in further inhibition of PNS-induced neurogenic vasodilation without affecting the vasodilator response to the CGRP injection. CGRP [8-37] but not AM [22-52] antagonized vasodilation induced by AM perfusion. These findings suggest that AM presynaptically inhibits neurotransmission of CGRPergic nerves, probably decreasing CGRP release, via receptors different from CGRP receptors. SN - 0196-9781 UR - https://www.unboundmedicine.com/medline/citation/11754977/Adrenomedullin_inhibits_neurotransmission_of_calcitonin_gene_related_peptide__CGRP__containing_vasodilator_nerves_in_rat_mesenteric_resistance_arteries_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0196-9781(01)00511-3 DB - PRIME DP - Unbound Medicine ER -