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Opposite influences of endogenous dopamine D1 and D2 receptor activation on activity states and electrophysiological properties of striatal neurons: studies combining in vivo intracellular recordings and reverse microdialysis.
J Neurosci. 2002 Jan 01; 22(1):294-304.JN

Abstract

The tonic influence of dopamine D1 and D2 receptors on the activity of striatal neurons in vivo was investigated by performing intracellular recordings concurrently with reverse microdialysis in chloral hydrate-anesthetized rats. Striatal neurons were recorded in the vicinity of the microdialysis probe to assess their activity during infusions of artificial CSF (aCSF), the D1 receptor antagonist SCH 23390 (10 microm), or the D2 receptor antagonist eticlopride (20 microm). SCH 23390 perfusion decreased the excitability of striatal neurons exhibiting electrophysiological characteristics of spiny projection cells as evidenced by a decrease in the maximal depolarized membrane potential, a decrease in the amplitude of up-state events, and an increase in the intracellular current injection amplitude required to elicit an action potential. Conversely, a marked depolarization of up- and down-state membrane potential modes, a decrease in the amplitude of intracellular current injection required to elicit an action potential, and an increase in the number of spikes evoked by depolarizing current steps were observed in striatal neurons after local eticlopride infusion. A significant increase in maximal EPSP amplitude evoked by electrical stimulation of the prefrontal cortex was also observed during local eticlopride but not SCH 23390 infusion. These results indicate that in intact systems, ongoing dopaminergic neurotransmission exerts a powerful tonic modulatory influence on the up- and down-state membrane properties of striatal neurons and controls their excitability differentially via both D1- and D2-like receptors. Moreover, a significant component of D2 receptor-mediated inhibition of striatal neuron activity in vivo occurs via suppression of excitatory synaptic transmission.

Authors+Show Affiliations

Department of Neuroscience, Center for Neuroscience, University of Pittsburgh, Pittsburgh, Pennsylvania 15260, USA. West@brain.bns.pitt.eduNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't
Research Support, U.S. Gov't, P.H.S.

Language

eng

PubMed ID

11756513

Citation

West, Anthony R., and Anthony A. Grace. "Opposite Influences of Endogenous Dopamine D1 and D2 Receptor Activation On Activity States and Electrophysiological Properties of Striatal Neurons: Studies Combining in Vivo Intracellular Recordings and Reverse Microdialysis." The Journal of Neuroscience : the Official Journal of the Society for Neuroscience, vol. 22, no. 1, 2002, pp. 294-304.
West AR, Grace AA. Opposite influences of endogenous dopamine D1 and D2 receptor activation on activity states and electrophysiological properties of striatal neurons: studies combining in vivo intracellular recordings and reverse microdialysis. J Neurosci. 2002;22(1):294-304.
West, A. R., & Grace, A. A. (2002). Opposite influences of endogenous dopamine D1 and D2 receptor activation on activity states and electrophysiological properties of striatal neurons: studies combining in vivo intracellular recordings and reverse microdialysis. The Journal of Neuroscience : the Official Journal of the Society for Neuroscience, 22(1), 294-304.
West AR, Grace AA. Opposite Influences of Endogenous Dopamine D1 and D2 Receptor Activation On Activity States and Electrophysiological Properties of Striatal Neurons: Studies Combining in Vivo Intracellular Recordings and Reverse Microdialysis. J Neurosci. 2002 Jan 1;22(1):294-304. PubMed PMID: 11756513.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Opposite influences of endogenous dopamine D1 and D2 receptor activation on activity states and electrophysiological properties of striatal neurons: studies combining in vivo intracellular recordings and reverse microdialysis. AU - West,Anthony R, AU - Grace,Anthony A, PY - 2002/1/5/pubmed PY - 2002/2/5/medline PY - 2002/1/5/entrez SP - 294 EP - 304 JF - The Journal of neuroscience : the official journal of the Society for Neuroscience JO - J Neurosci VL - 22 IS - 1 N2 - The tonic influence of dopamine D1 and D2 receptors on the activity of striatal neurons in vivo was investigated by performing intracellular recordings concurrently with reverse microdialysis in chloral hydrate-anesthetized rats. Striatal neurons were recorded in the vicinity of the microdialysis probe to assess their activity during infusions of artificial CSF (aCSF), the D1 receptor antagonist SCH 23390 (10 microm), or the D2 receptor antagonist eticlopride (20 microm). SCH 23390 perfusion decreased the excitability of striatal neurons exhibiting electrophysiological characteristics of spiny projection cells as evidenced by a decrease in the maximal depolarized membrane potential, a decrease in the amplitude of up-state events, and an increase in the intracellular current injection amplitude required to elicit an action potential. Conversely, a marked depolarization of up- and down-state membrane potential modes, a decrease in the amplitude of intracellular current injection required to elicit an action potential, and an increase in the number of spikes evoked by depolarizing current steps were observed in striatal neurons after local eticlopride infusion. A significant increase in maximal EPSP amplitude evoked by electrical stimulation of the prefrontal cortex was also observed during local eticlopride but not SCH 23390 infusion. These results indicate that in intact systems, ongoing dopaminergic neurotransmission exerts a powerful tonic modulatory influence on the up- and down-state membrane properties of striatal neurons and controls their excitability differentially via both D1- and D2-like receptors. Moreover, a significant component of D2 receptor-mediated inhibition of striatal neuron activity in vivo occurs via suppression of excitatory synaptic transmission. SN - 1529-2401 UR - https://www.unboundmedicine.com/medline/citation/11756513/Opposite_influences_of_endogenous_dopamine_D1_and_D2_receptor_activation_on_activity_states_and_electrophysiological_properties_of_striatal_neurons:_studies_combining_in_vivo_intracellular_recordings_and_reverse_microdialysis_ L2 - http://www.jneurosci.org/cgi/pmidlookup?view=long&pmid=11756513 DB - PRIME DP - Unbound Medicine ER -