Tags

Type your tag names separated by a space and hit enter

Composition of LDL as determinant of its susceptibility to in vitro oxidation in patients with well-controlled type 2 diabetes.

Abstract

BACKGROUND

There is increasing evidence that oxidation of low-density lipoprotein (LDL) in the vascular wall plays an important role in the development of atherosclerosis. The present study was undertaken to characterise how different constituents of LDL contribute to its in vitro oxidisability.

METHODS

The LDL composition, i.e. lipids, antioxidants, fatty acids, plasmenylcholines, and baseline level of conjugated dienes (CD) of 94 well-controlled and normolipidaemic type 2 diabetic patients was measured. Two oxidisability indices were determined: the lag time, reflecting the resistance of LDL to copper-induced oxidation, and the amount of conjugated dienes formed during oxidation of LDL.

RESULTS

The lag time was not related to the total level of saturated, monounsaturated, and polyunsaturated fatty acids, but a strong inverse relationship was observed with fatty acids with three or more double bonds (r = -0.56, p < 0.001). In addition, an inverse relation was observed between the lag time and LDL-plasmenylcholine (r = -0.35, p < 0.001). Although not related to lag time in univariate analysis, alpha-tocopherol was a significant determinant in multiple regression analysis. A multiple linear regression model with LDL polyunsaturated fatty acids with three or more double bonds, alpha-tocopherol, monounsaturated fatty acids, and plasmenylcholines as determinants explained 47% of the variation in lag time. CD production was negatively correlated to oleic acid and positively to linoleic acid (r = -0.45 and r = 0.73, respectively; p<0.001).

CONCLUSIONS

Fatty acids with three or more double bonds were the most important predictor of LDL lag time, whereas oleic acid and linoleic acid were major determinants of the amount of CD formed during oxidation of LDL.

Links

  • Publisher Full Text
  • Authors+Show Affiliations

    ,

    Metabolic Unit, Department of Clinical Chemistry, University Hospital Vrije Universiteit, Amsterdam, The Netherlands. pscheffer@azvu.nl

    , , , ,

    Source

    MeSH

    Cholesterol
    Cholesterol Esters
    Copper
    Diabetes Mellitus, Type 2
    Fatty Acids
    Female
    Humans
    Linear Models
    Lipoproteins, LDL
    Male
    Middle Aged
    Multivariate Analysis
    Oxidation-Reduction
    Phospholipids
    Triglycerides
    Ubiquinone
    alpha-Tocopherol

    Pub Type(s)

    Journal Article

    Language

    eng

    PubMed ID

    11757082

    Citation

    Scheffer, P G., et al. "Composition of LDL as Determinant of Its Susceptibility to in Vitro Oxidation in Patients With Well-controlled Type 2 Diabetes." Diabetes/metabolism Research and Reviews, vol. 17, no. 6, 2001, pp. 459-66.
    Scheffer PG, Bakker SJ, Popp-Snijders C, et al. Composition of LDL as determinant of its susceptibility to in vitro oxidation in patients with well-controlled type 2 diabetes. Diabetes Metab Res Rev. 2001;17(6):459-66.
    Scheffer, P. G., Bakker, S. J., Popp-Snijders, C., Heine, R. J., Schutgens, R. B., & Teerlink, T. (2001). Composition of LDL as determinant of its susceptibility to in vitro oxidation in patients with well-controlled type 2 diabetes. Diabetes/metabolism Research and Reviews, 17(6), pp. 459-66.
    Scheffer PG, et al. Composition of LDL as Determinant of Its Susceptibility to in Vitro Oxidation in Patients With Well-controlled Type 2 Diabetes. Diabetes Metab Res Rev. 2001;17(6):459-66. PubMed PMID: 11757082.
    * Article titles in AMA citation format should be in sentence-case
    TY - JOUR T1 - Composition of LDL as determinant of its susceptibility to in vitro oxidation in patients with well-controlled type 2 diabetes. AU - Scheffer,P G, AU - Bakker,S J, AU - Popp-Snijders,C, AU - Heine,R J, AU - Schutgens,R B, AU - Teerlink,T, PY - 2002/1/5/pubmed PY - 2002/3/30/medline PY - 2002/1/5/entrez SP - 459 EP - 66 JF - Diabetes/metabolism research and reviews JO - Diabetes Metab. Res. Rev. VL - 17 IS - 6 N2 - BACKGROUND: There is increasing evidence that oxidation of low-density lipoprotein (LDL) in the vascular wall plays an important role in the development of atherosclerosis. The present study was undertaken to characterise how different constituents of LDL contribute to its in vitro oxidisability. METHODS: The LDL composition, i.e. lipids, antioxidants, fatty acids, plasmenylcholines, and baseline level of conjugated dienes (CD) of 94 well-controlled and normolipidaemic type 2 diabetic patients was measured. Two oxidisability indices were determined: the lag time, reflecting the resistance of LDL to copper-induced oxidation, and the amount of conjugated dienes formed during oxidation of LDL. RESULTS: The lag time was not related to the total level of saturated, monounsaturated, and polyunsaturated fatty acids, but a strong inverse relationship was observed with fatty acids with three or more double bonds (r = -0.56, p < 0.001). In addition, an inverse relation was observed between the lag time and LDL-plasmenylcholine (r = -0.35, p < 0.001). Although not related to lag time in univariate analysis, alpha-tocopherol was a significant determinant in multiple regression analysis. A multiple linear regression model with LDL polyunsaturated fatty acids with three or more double bonds, alpha-tocopherol, monounsaturated fatty acids, and plasmenylcholines as determinants explained 47% of the variation in lag time. CD production was negatively correlated to oleic acid and positively to linoleic acid (r = -0.45 and r = 0.73, respectively; p<0.001). CONCLUSIONS: Fatty acids with three or more double bonds were the most important predictor of LDL lag time, whereas oleic acid and linoleic acid were major determinants of the amount of CD formed during oxidation of LDL. SN - 1520-7552 UR - https://www.unboundmedicine.com/medline/citation/11757082/Composition_of_LDL_as_determinant_of_its_susceptibility_to_in_vitro_oxidation_in_patients_with_well_controlled_type_2_diabetes_ L2 - https://onlinelibrary.wiley.com/resolve/openurl?genre=article&amp;sid=nlm:pubmed&amp;issn=1520-7552&amp;date=2001&amp;volume=17&amp;issue=6&amp;spage=459 DB - PRIME DP - Unbound Medicine ER -