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[An application value of detecting K-ras and p53 gene mutation in the stool and pure pancreatic juice for diagnosis of early pancreatic cancer].
Zhonghua Yi Xue Za Zhi 2001; 81(17):1050-3ZY

Abstract

OBJECTIVE

To explore new methods for the early diagnosis of pancreatic cancer through detecting of K-ras, p53 mutations in pancreatic juice and stool.

METHODS

201 patients in PUMC Hospital from 1994-2000. 5 and 60-control individuals were enrolled. K-ras point mutations were detected by PCR-RFLP, however p53 mutation was detected by PCR-SSCP.

RESULTS

K-ras mutations in pancreatic juice were found in 87.8% (36/41) of pancreatic cancer, 23.5% (4/17) of benign pancreatic disease. Of 261 stools specimens, amplification was successful in 235 (90.0%). K-ras mutation in stool were found in 88.0% (66/75) of pancreatic cancer 51.1% (24/47) of benign pancreatic disease, 19.6% (9/46) of normal individuals. p53 mutation in pancreatic juice were found in 47.4% (18/38), 12.5% (2/16) of benign pancreatic disease, p53 mutation in stool were found in 37.1% (23/62), 19.1% (4/12) of chronic pancreatitis.

CONCLUSION

K-ras mutation in pancreatic juice has high sensitivity and specificity, so it can be used as a adjunct in the diagnosis of pancreatic cancer. Detection of K-ras mutation combined with p53 mutation in stool can be helpful to screen for pancreatic carcinoma. Combined with serum CA19-9 detection, it might increase the early diagnostic rate of pancreatic carcinoma.

Authors+Show Affiliations

Peking Union Medical Hospital, Chinese Academy of Medical Science, Peking Union Medical College, Beijing 100730, China.No affiliation info availableNo affiliation info available

Pub Type(s)

English Abstract
Journal Article

Language

chi

PubMed ID

11758254

Citation

Lu, X, et al. "[An Application Value of Detecting K-ras and P53 Gene Mutation in the Stool and Pure Pancreatic Juice for Diagnosis of Early Pancreatic Cancer]." Zhonghua Yi Xue Za Zhi, vol. 81, no. 17, 2001, pp. 1050-3.
Lu X, Xu T, Qian J. [An application value of detecting K-ras and p53 gene mutation in the stool and pure pancreatic juice for diagnosis of early pancreatic cancer]. Zhonghua Yi Xue Za Zhi. 2001;81(17):1050-3.
Lu, X., Xu, T., & Qian, J. (2001). [An application value of detecting K-ras and p53 gene mutation in the stool and pure pancreatic juice for diagnosis of early pancreatic cancer]. Zhonghua Yi Xue Za Zhi, 81(17), pp. 1050-3.
Lu X, Xu T, Qian J. [An Application Value of Detecting K-ras and P53 Gene Mutation in the Stool and Pure Pancreatic Juice for Diagnosis of Early Pancreatic Cancer]. Zhonghua Yi Xue Za Zhi. 2001 Sep 10;81(17):1050-3. PubMed PMID: 11758254.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - [An application value of detecting K-ras and p53 gene mutation in the stool and pure pancreatic juice for diagnosis of early pancreatic cancer]. AU - Lu,X, AU - Xu,T, AU - Qian,J, PY - 2002/1/5/pubmed PY - 2002/1/5/medline PY - 2002/1/5/entrez SP - 1050 EP - 3 JF - Zhonghua yi xue za zhi JO - Zhonghua Yi Xue Za Zhi VL - 81 IS - 17 N2 - OBJECTIVE: To explore new methods for the early diagnosis of pancreatic cancer through detecting of K-ras, p53 mutations in pancreatic juice and stool. METHODS: 201 patients in PUMC Hospital from 1994-2000. 5 and 60-control individuals were enrolled. K-ras point mutations were detected by PCR-RFLP, however p53 mutation was detected by PCR-SSCP. RESULTS: K-ras mutations in pancreatic juice were found in 87.8% (36/41) of pancreatic cancer, 23.5% (4/17) of benign pancreatic disease. Of 261 stools specimens, amplification was successful in 235 (90.0%). K-ras mutation in stool were found in 88.0% (66/75) of pancreatic cancer 51.1% (24/47) of benign pancreatic disease, 19.6% (9/46) of normal individuals. p53 mutation in pancreatic juice were found in 47.4% (18/38), 12.5% (2/16) of benign pancreatic disease, p53 mutation in stool were found in 37.1% (23/62), 19.1% (4/12) of chronic pancreatitis. CONCLUSION: K-ras mutation in pancreatic juice has high sensitivity and specificity, so it can be used as a adjunct in the diagnosis of pancreatic cancer. Detection of K-ras mutation combined with p53 mutation in stool can be helpful to screen for pancreatic carcinoma. Combined with serum CA19-9 detection, it might increase the early diagnostic rate of pancreatic carcinoma. SN - 0376-2491 UR - https://www.unboundmedicine.com/medline/citation/11758254/[An_application_value_of_detecting_K_ras_and_p53_gene_mutation_in_the_stool_and_pure_pancreatic_juice_for_diagnosis_of_early_pancreatic_cancer]_ L2 - http://journal.yiigle.com/LinkIn.do?linkin_type=pubmed&issn=0376-2491&year=2001&vol=81&issue=17&fpage=1050 DB - PRIME DP - Unbound Medicine ER -