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[An application value of detecting K-ras and p53 gene mutation in the stool and pure pancreatic juice for diagnosis of early pancreatic cancer].
Zhonghua Yi Xue Za Zhi. 2001 Sep 10; 81(17):1050-3.ZY

Abstract

OBJECTIVE

To explore new methods for the early diagnosis of pancreatic cancer through detecting of K-ras, p53 mutations in pancreatic juice and stool.

METHODS

201 patients in PUMC Hospital from 1994-2000. 5 and 60-control individuals were enrolled. K-ras point mutations were detected by PCR-RFLP, however p53 mutation was detected by PCR-SSCP.

RESULTS

K-ras mutations in pancreatic juice were found in 87.8% (36/41) of pancreatic cancer, 23.5% (4/17) of benign pancreatic disease. Of 261 stools specimens, amplification was successful in 235 (90.0%). K-ras mutation in stool were found in 88.0% (66/75) of pancreatic cancer 51.1% (24/47) of benign pancreatic disease, 19.6% (9/46) of normal individuals. p53 mutation in pancreatic juice were found in 47.4% (18/38), 12.5% (2/16) of benign pancreatic disease, p53 mutation in stool were found in 37.1% (23/62), 19.1% (4/12) of chronic pancreatitis.

CONCLUSION

K-ras mutation in pancreatic juice has high sensitivity and specificity, so it can be used as a adjunct in the diagnosis of pancreatic cancer. Detection of K-ras mutation combined with p53 mutation in stool can be helpful to screen for pancreatic carcinoma. Combined with serum CA19-9 detection, it might increase the early diagnostic rate of pancreatic carcinoma.

Links

  • Publisher Full Text

    • Authors+Show Affiliations

    Lu X
    Peking Union Medical Hospital, Chinese Academy of Medical Science, Peking Union Medical College, Beijing 100730, China.
    Xu T
    No affiliation info available
    Qian J
    No affiliation info available

    MeSH

    AdultAgedFecesFemaleGenes, p53Genes, rasHumansMaleMiddle AgedMutationPancreatic JuicePancreatic NeoplasmsPolymerase Chain ReactionPolymorphism, Restriction Fragment LengthPolymorphism, Single-Stranded Conformational

    Pub Type(s)

    English Abstract
    Journal Article

    Language

    chi

    PubMed ID

    11758254

    Citation

    Lu, X, et al. "[An Application Value of Detecting K-ras and P53 Gene Mutation in the Stool and Pure Pancreatic Juice for Diagnosis of Early Pancreatic Cancer]." Zhonghua Yi Xue Za Zhi, vol. 81, no. 17, 2001, pp. 1050-3.
    Lu X, Xu T, Qian J. [An application value of detecting K-ras and p53 gene mutation in the stool and pure pancreatic juice for diagnosis of early pancreatic cancer]. Zhonghua Yi Xue Za Zhi. 2001;81(17):1050-3.
    Lu, X., Xu, T., & Qian, J. (2001). [An application value of detecting K-ras and p53 gene mutation in the stool and pure pancreatic juice for diagnosis of early pancreatic cancer]. Zhonghua Yi Xue Za Zhi, 81(17), 1050-3.
    Lu X, Xu T, Qian J. [An Application Value of Detecting K-ras and P53 Gene Mutation in the Stool and Pure Pancreatic Juice for Diagnosis of Early Pancreatic Cancer]. Zhonghua Yi Xue Za Zhi. 2001 Sep 10;81(17):1050-3. PubMed PMID: 11758254.
    * Article titles in AMA citation format should be in sentence-case
    TY - JOUR T1 - [An application value of detecting K-ras and p53 gene mutation in the stool and pure pancreatic juice for diagnosis of early pancreatic cancer]. AU - Lu,X, AU - Xu,T, AU - Qian,J, PY - 2002/1/5/pubmed PY - 2002/1/5/medline PY - 2002/1/5/entrez SP - 1050 EP - 3 JF - Zhonghua yi xue za zhi JO - Zhonghua Yi Xue Za Zhi VL - 81 IS - 17 N2 - OBJECTIVE: To explore new methods for the early diagnosis of pancreatic cancer through detecting of K-ras, p53 mutations in pancreatic juice and stool. METHODS: 201 patients in PUMC Hospital from 1994-2000. 5 and 60-control individuals were enrolled. K-ras point mutations were detected by PCR-RFLP, however p53 mutation was detected by PCR-SSCP. RESULTS: K-ras mutations in pancreatic juice were found in 87.8% (36/41) of pancreatic cancer, 23.5% (4/17) of benign pancreatic disease. Of 261 stools specimens, amplification was successful in 235 (90.0%). K-ras mutation in stool were found in 88.0% (66/75) of pancreatic cancer 51.1% (24/47) of benign pancreatic disease, 19.6% (9/46) of normal individuals. p53 mutation in pancreatic juice were found in 47.4% (18/38), 12.5% (2/16) of benign pancreatic disease, p53 mutation in stool were found in 37.1% (23/62), 19.1% (4/12) of chronic pancreatitis. CONCLUSION: K-ras mutation in pancreatic juice has high sensitivity and specificity, so it can be used as a adjunct in the diagnosis of pancreatic cancer. Detection of K-ras mutation combined with p53 mutation in stool can be helpful to screen for pancreatic carcinoma. Combined with serum CA19-9 detection, it might increase the early diagnostic rate of pancreatic carcinoma. SN - 0376-2491 UR - https://www.unboundmedicine.com/medline/citation/11758254/[An_application_value_of_detecting_K_ras_and_p53_gene_mutation_in_the_stool_and_pure_pancreatic_juice_for_diagnosis_of_early_pancreatic_cancer]_ L2 - http://journal.yiigle.com/LinkIn.do?linkin_type=pubmed&issn=0376-2491&year=2001&vol=81&issue=17&fpage=1050 DB - PRIME DP - Unbound Medicine ER -