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Cometabolic biosynthesis of copolyesters consisting of 3-hydroxyvalerate and medium-chain-length 3-hydroxyalkanoates by Pseudomonas sp. DSY-82.
Antonie Van Leeuwenhoek. 2001 Oct; 80(2):185-91.AV

Abstract

A newly isolated strain, designated as Pseudomonas sp. DSY-82, synthesized medium-chain-length polyhydroxyalkanoate (MCL-PHA) copolyesters when grown on alkanoates from hexanoate to undecanoate as the sole carbon source. When used alone, butyrate and valerate supported the growth of the isolate but not PHA production. However, unusual polyesters containing 3-hydroxyvalerate, as well as various MCL 3-hydroxyalkanoate monomeric units, were synthesized when valerate was cofed with either nonanoate or 10-undecenoate, suggesting the formation of monomer units from both substrates. Concentrations of 3-hydroxyvalerate, 3-hydroxyoctanoate, and 3-hydroxydecanoate in the PHAs produced were significantly elevated by the addition of valerate, indicating that the incorporation of these monomer units to PHA occurred primarily through cometabolism. The total amount of these monomer units in the PHAs reached up to 30%. The PHAs produced in this study were most likely random copolyesters as determined by differential scanning calorimetric analysis. This is the first case of microbial synthesis of copolyesters consisting of 3-hydroxyvalerate and MCL 3-hydroxyalkanoate monomer units through cometabolism.

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Authors+Show Affiliations

Kang HO
Department of Microbiology, Chungnam National University, Daejeon, Korea.
Chung CW
No affiliation info available
Kim HW
No affiliation info available
Kim YB
No affiliation info available
Rhee YH
No affiliation info available

MeSH

AerobiosisCulture MediaFatty Acids, NonesterifiedHydroxy AcidsPentanoic AcidsPolyestersPseudomonas

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

11759051

Citation

Kang, H O., et al. "Cometabolic Biosynthesis of Copolyesters Consisting of 3-hydroxyvalerate and Medium-chain-length 3-hydroxyalkanoates By Pseudomonas Sp. DSY-82." Antonie Van Leeuwenhoek, vol. 80, no. 2, 2001, pp. 185-91.
Kang HO, Chung CW, Kim HW, et al. Cometabolic biosynthesis of copolyesters consisting of 3-hydroxyvalerate and medium-chain-length 3-hydroxyalkanoates by Pseudomonas sp. DSY-82. Antonie Van Leeuwenhoek. 2001;80(2):185-91.
Kang, H. O., Chung, C. W., Kim, H. W., Kim, Y. B., & Rhee, Y. H. (2001). Cometabolic biosynthesis of copolyesters consisting of 3-hydroxyvalerate and medium-chain-length 3-hydroxyalkanoates by Pseudomonas sp. DSY-82. Antonie Van Leeuwenhoek, 80(2), 185-91.
Kang HO, et al. Cometabolic Biosynthesis of Copolyesters Consisting of 3-hydroxyvalerate and Medium-chain-length 3-hydroxyalkanoates By Pseudomonas Sp. DSY-82. Antonie Van Leeuwenhoek. 2001;80(2):185-91. PubMed PMID: 11759051.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Cometabolic biosynthesis of copolyesters consisting of 3-hydroxyvalerate and medium-chain-length 3-hydroxyalkanoates by Pseudomonas sp. DSY-82. AU - Kang,H O, AU - Chung,C W, AU - Kim,H W, AU - Kim,Y B, AU - Rhee,Y H, PY - 2002/1/5/pubmed PY - 2002/5/9/medline PY - 2002/1/5/entrez SP - 185 EP - 91 JF - Antonie van Leeuwenhoek JO - Antonie Van Leeuwenhoek VL - 80 IS - 2 N2 - A newly isolated strain, designated as Pseudomonas sp. DSY-82, synthesized medium-chain-length polyhydroxyalkanoate (MCL-PHA) copolyesters when grown on alkanoates from hexanoate to undecanoate as the sole carbon source. When used alone, butyrate and valerate supported the growth of the isolate but not PHA production. However, unusual polyesters containing 3-hydroxyvalerate, as well as various MCL 3-hydroxyalkanoate monomeric units, were synthesized when valerate was cofed with either nonanoate or 10-undecenoate, suggesting the formation of monomer units from both substrates. Concentrations of 3-hydroxyvalerate, 3-hydroxyoctanoate, and 3-hydroxydecanoate in the PHAs produced were significantly elevated by the addition of valerate, indicating that the incorporation of these monomer units to PHA occurred primarily through cometabolism. The total amount of these monomer units in the PHAs reached up to 30%. The PHAs produced in this study were most likely random copolyesters as determined by differential scanning calorimetric analysis. This is the first case of microbial synthesis of copolyesters consisting of 3-hydroxyvalerate and MCL 3-hydroxyalkanoate monomer units through cometabolism. SN - 0003-6072 UR - https://www.unboundmedicine.com/medline/citation/11759051/Cometabolic_biosynthesis_of_copolyesters_consisting_of_3_hydroxyvalerate_and_medium_chain_length_3_hydroxyalkanoates_by_Pseudomonas_sp__DSY_82_ L2 - http://ovidsp.ovid.com/ovidweb.cgi?T=JS&PAGE=linkout&SEARCH=11759051.ui DB - PRIME DP - Unbound Medicine ER -