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Suppressive effects of genistein dosage and resistance exercise on bone loss in ovariectomized rats.

Abstract

This study was designed to determine whether combined treatments with genistein dosage and moderate resistance exercise would exhibit synergistically preventive effects on bone loss following the onset of menopause. Forty-one 12 wk-old female SD rats were assigned to five groups: 1) Sham operated (Sham); 2) ovariectomized (OVX-Cont); 3) OVX received genistein (OVX-GEN); 4) OVX exercised (OVX-EXE); and 5) OVX treated with both genistein and exercise (OVX-GEN-EXE). All rats were fed a low Ca (0.1%) diet ad libitum. Daily genistein dosage was 12 mg/kg body weight. Exercising rats took 40 sets of 1-min run interspersed with 1-min rest with a 100 g weight on the back on an uphill treadmill at 20 m/min. The experimental duration consisted of the adaptation and treatment periods of 4 weeks each. Uterine weight in OVX-Cont, OVX-GEN, OVX-EXE and OVX-GEN-EXE decreased to about 15% of that in Sham (p < 0.001). The femoral BMD (mg/cm2; mean +/- SE), assessed by DEXA (Lunar), of OVX-Cont was significantly lowered to 206 +/- 5 by -9%, as compared to 226 +/- 2 of Sham (p < 0.001). The BMD of OVX-GEN, OVX-EXE and OVX-GEN-EXE were 217 +/- 2, 217 +/- 2 and 222 +/- 2, respectively, and genistein dosage and resistance exercise equally increased the BMD of OVX rats by 5% (p < 0.01). Combined treatment of genistein and exercise more successfully recovered their decreased BMD by 8% (p < 0.001). BMD of the fourth lumbar vertebrae in OVX-Cont was declined to 191 +/- 7 by -15%, as compared to 225 +/- 4 in Sham (p < 0.001). OVX-EXE and OVX-GEN-EXE gained the BMD by 6% to 205 +/- 4 and 203 +/- 3, respectively, as compared to that of OVX-Cont (p < 0.01). These results suggest the possibility that the combined treatment of genistein dosage and resistance exercise have more beneficial effects by acting rather independently than their separate trials on the prevention of ovx-induced bone loss in femurs.

Authors+Show Affiliations

Department of Sports Sciences, School of Human Sciences, Waseda University. daito@human.waseda.ac.jpNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article

Language

eng

PubMed ID

11759267

Citation

Nakajima, D, et al. "Suppressive Effects of Genistein Dosage and Resistance Exercise On Bone Loss in Ovariectomized Rats." Journal of Physiological Anthropology and Applied Human Science, vol. 20, no. 5, 2001, pp. 285-91.
Nakajima D, Kim CS, Oh TW, et al. Suppressive effects of genistein dosage and resistance exercise on bone loss in ovariectomized rats. J Physiol Anthropol Appl Human Sci. 2001;20(5):285-91.
Nakajima, D., Kim, C. S., Oh, T. W., Yang, C. Y., Naka, T., Igawa, S., & Ohta, F. (2001). Suppressive effects of genistein dosage and resistance exercise on bone loss in ovariectomized rats. Journal of Physiological Anthropology and Applied Human Science, 20(5), pp. 285-91.
Nakajima D, et al. Suppressive Effects of Genistein Dosage and Resistance Exercise On Bone Loss in Ovariectomized Rats. J Physiol Anthropol Appl Human Sci. 2001;20(5):285-91. PubMed PMID: 11759267.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Suppressive effects of genistein dosage and resistance exercise on bone loss in ovariectomized rats. AU - Nakajima,D, AU - Kim,C S, AU - Oh,T W, AU - Yang,C Y, AU - Naka,T, AU - Igawa,S, AU - Ohta,F, PY - 2002/1/5/pubmed PY - 2002/1/16/medline PY - 2002/1/5/entrez SP - 285 EP - 91 JF - Journal of physiological anthropology and applied human science JO - J Physiol Anthropol Appl Human Sci VL - 20 IS - 5 N2 - This study was designed to determine whether combined treatments with genistein dosage and moderate resistance exercise would exhibit synergistically preventive effects on bone loss following the onset of menopause. Forty-one 12 wk-old female SD rats were assigned to five groups: 1) Sham operated (Sham); 2) ovariectomized (OVX-Cont); 3) OVX received genistein (OVX-GEN); 4) OVX exercised (OVX-EXE); and 5) OVX treated with both genistein and exercise (OVX-GEN-EXE). All rats were fed a low Ca (0.1%) diet ad libitum. Daily genistein dosage was 12 mg/kg body weight. Exercising rats took 40 sets of 1-min run interspersed with 1-min rest with a 100 g weight on the back on an uphill treadmill at 20 m/min. The experimental duration consisted of the adaptation and treatment periods of 4 weeks each. Uterine weight in OVX-Cont, OVX-GEN, OVX-EXE and OVX-GEN-EXE decreased to about 15% of that in Sham (p < 0.001). The femoral BMD (mg/cm2; mean +/- SE), assessed by DEXA (Lunar), of OVX-Cont was significantly lowered to 206 +/- 5 by -9%, as compared to 226 +/- 2 of Sham (p < 0.001). The BMD of OVX-GEN, OVX-EXE and OVX-GEN-EXE were 217 +/- 2, 217 +/- 2 and 222 +/- 2, respectively, and genistein dosage and resistance exercise equally increased the BMD of OVX rats by 5% (p < 0.01). Combined treatment of genistein and exercise more successfully recovered their decreased BMD by 8% (p < 0.001). BMD of the fourth lumbar vertebrae in OVX-Cont was declined to 191 +/- 7 by -15%, as compared to 225 +/- 4 in Sham (p < 0.001). OVX-EXE and OVX-GEN-EXE gained the BMD by 6% to 205 +/- 4 and 203 +/- 3, respectively, as compared to that of OVX-Cont (p < 0.01). These results suggest the possibility that the combined treatment of genistein dosage and resistance exercise have more beneficial effects by acting rather independently than their separate trials on the prevention of ovx-induced bone loss in femurs. SN - 1345-3475 UR - https://www.unboundmedicine.com/medline/citation/11759267/Suppressive_effects_of_genistein_dosage_and_resistance_exercise_on_bone_loss_in_ovariectomized_rats_ L2 - http://joi.jlc.jst.go.jp/JST.JSTAGE/jpa/20.285?from=PubMed DB - PRIME DP - Unbound Medicine ER -