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[Relationship between gene polymorphism at the apolipoprotein E locus and serum lipid profile in urban children of school age in Beijing].
Zhonghua Yu Fang Yi Xue Za Zhi 2001; 35(5):297-300ZY

Abstract

OBJECTIVE

To study the relationship between gene polymorphism at the apolipoprotein E (ApoE) locus and serum lipid levels in children of school age.

METHODS

Blood samples were collected from 307 normal children aged 7 to 11 years, including 150 boys and 157 girls, and their serum lipid profile, including total cholesterol (TC), triglyceride (TG), low-density lipoprotein-cholesterol (LDL-C), high-density lipoprotein-cholesterol (HDL-C), apolipoprotein B(apoB), apoprotein A1 (ApoA I) and lipoptoein alpha[LP (alpha)], were detected and their ApoE-Hha I gene polymorphism were tested by the PCR-RFLP method.

RESULTS

The detection rates for the most frequent genotype ApoE3/3 was 54.7%, other genotypes E4/3, E4/4, E3/2, E4/2 and E2/2 were 23.8%, 9.1%, 8.1%, 3.9% and 0.3%, respectively, in 307 children, with no significant difference between girls and boys(P > 0.05). Frequency of the often seen allele E3 was 70.7%, and that of E4 and E2 was 23.0% and 6.4, respectively, with E4 significantly higher than and E2 similar to that in the other domestic reports. The levels of TC, LDL-C, ApoA I and ApoB were higher in boys with genotypes E4/3 and E3/3 than those with genotypes E3/2 and E4/2. Levels of TC and ApoA I in the boys with genotype E3/2 were higher, with TC of 4.28 mmol/L and ApoA I of 3.41 mmol/L. There were no significant difference in serum lipid levels of girls with different genotypes(P > 0.05). There also was no significant difference in the ApoE-Hha I genotype distribution between normal children and those with high TC. ApoE2 could decrease the level of TC by 0.377 mmol/L, and LDL-C by 0.329 mmol/L.

CONCLUSIONS

Serum level of TC associated with polymorphism of ApoE gene carrier in boys, with the lowest levels of TC, LDL-C and ApoE in three with allele E2.

Authors+Show Affiliations

Institute of Child and Adolescent Health, Peking University, Beijing 100083, China.No affiliation info availableNo affiliation info available

Pub Type(s)

English Abstract
Journal Article

Language

chi

PubMed ID

11769625

Citation

Zhu, W, et al. "[Relationship Between Gene Polymorphism at the Apolipoprotein E Locus and Serum Lipid Profile in Urban Children of School Age in Beijing]." Zhonghua Yu Fang Yi Xue Za Zhi [Chinese Journal of Preventive Medicine], vol. 35, no. 5, 2001, pp. 297-300.
Zhu W, Feng N, Wang Y. [Relationship between gene polymorphism at the apolipoprotein E locus and serum lipid profile in urban children of school age in Beijing]. Zhonghua Yu Fang Yi Xue Za Zhi. 2001;35(5):297-300.
Zhu, W., Feng, N., & Wang, Y. (2001). [Relationship between gene polymorphism at the apolipoprotein E locus and serum lipid profile in urban children of school age in Beijing]. Zhonghua Yu Fang Yi Xue Za Zhi [Chinese Journal of Preventive Medicine], 35(5), pp. 297-300.
Zhu W, Feng N, Wang Y. [Relationship Between Gene Polymorphism at the Apolipoprotein E Locus and Serum Lipid Profile in Urban Children of School Age in Beijing]. Zhonghua Yu Fang Yi Xue Za Zhi. 2001;35(5):297-300. PubMed PMID: 11769625.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - [Relationship between gene polymorphism at the apolipoprotein E locus and serum lipid profile in urban children of school age in Beijing]. AU - Zhu,W, AU - Feng,N, AU - Wang,Y, PY - 2002/1/5/pubmed PY - 2002/3/5/medline PY - 2002/1/5/entrez SP - 297 EP - 300 JF - Zhonghua yu fang yi xue za zhi [Chinese journal of preventive medicine] JO - Zhonghua Yu Fang Yi Xue Za Zhi VL - 35 IS - 5 N2 - OBJECTIVE: To study the relationship between gene polymorphism at the apolipoprotein E (ApoE) locus and serum lipid levels in children of school age. METHODS: Blood samples were collected from 307 normal children aged 7 to 11 years, including 150 boys and 157 girls, and their serum lipid profile, including total cholesterol (TC), triglyceride (TG), low-density lipoprotein-cholesterol (LDL-C), high-density lipoprotein-cholesterol (HDL-C), apolipoprotein B(apoB), apoprotein A1 (ApoA I) and lipoptoein alpha[LP (alpha)], were detected and their ApoE-Hha I gene polymorphism were tested by the PCR-RFLP method. RESULTS: The detection rates for the most frequent genotype ApoE3/3 was 54.7%, other genotypes E4/3, E4/4, E3/2, E4/2 and E2/2 were 23.8%, 9.1%, 8.1%, 3.9% and 0.3%, respectively, in 307 children, with no significant difference between girls and boys(P > 0.05). Frequency of the often seen allele E3 was 70.7%, and that of E4 and E2 was 23.0% and 6.4, respectively, with E4 significantly higher than and E2 similar to that in the other domestic reports. The levels of TC, LDL-C, ApoA I and ApoB were higher in boys with genotypes E4/3 and E3/3 than those with genotypes E3/2 and E4/2. Levels of TC and ApoA I in the boys with genotype E3/2 were higher, with TC of 4.28 mmol/L and ApoA I of 3.41 mmol/L. There were no significant difference in serum lipid levels of girls with different genotypes(P > 0.05). There also was no significant difference in the ApoE-Hha I genotype distribution between normal children and those with high TC. ApoE2 could decrease the level of TC by 0.377 mmol/L, and LDL-C by 0.329 mmol/L. CONCLUSIONS: Serum level of TC associated with polymorphism of ApoE gene carrier in boys, with the lowest levels of TC, LDL-C and ApoE in three with allele E2. SN - 0253-9624 UR - https://www.unboundmedicine.com/medline/citation/11769625/[Relationship_between_gene_polymorphism_at_the_apolipoprotein_E_locus_and_serum_lipid_profile_in_urban_children_of_school_age_in_Beijing]_ DB - PRIME DP - Unbound Medicine ER -