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Detection of measles virus-specific immunoglobulin M in dried venous blood samples by using a commercial enzyme immunoassay.
J Clin Microbiol. 2002 Jan; 40(1):5-9.JC

Abstract

The optical densities (ODs) of 216 dried venous blood (DVB) samples submitted to the Victorian Infectious Diseases Reference Laboratory as part of enhanced measles surveillance were compared to the ODs of the corresponding serum samples collected at the same time. DVB samples, stored for up to 24 months at 4 degrees C, were tested by the Dade Behring Enzygnost Anti-Measles-Virus/IgM immunoassay. Elution and testing conditions were optimized with the use of spiked DVB samples. The assay showed an overall sensitivity of 90.2% and a specificity of 98.8% for DVB samples compared to the results for serum. When the results were analyzed according to the length of time that the DVB sample had been stored, the assay was 100% sensitive and 97% specific according to the ODs for those samples stored for less than 6 months compared to the results for the corresponding serum samples, with 97.7% agreement between the results for the two sample types. These results demonstrate the potential for the use of DVB samples for the diagnosis of measles in routine diagnostic laboratories.

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Authors+Show Affiliations

Riddell MA
Victorian Infectious Diseases Reference Laboratory/WHO Western Pacific Measles Regional Reference Laboratory, North Melbourne, Australia. michaela.riddell@mh.org.au
Leydon JA
No affiliation info available
Catton MG
No affiliation info available
Kelly HA
No affiliation info available

MeSH

AdolescentAntibodies, ViralAntibody SpecificityBlood Specimen CollectionHumansImmunoenzyme TechniquesImmunoglobulin MMeaslesMeasles virusReagent Kits, DiagnosticSensitivity and SpecificityVeins

Pub Type(s)

Evaluation Study
Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

11773084

Citation

Riddell, Michaela A., et al. "Detection of Measles Virus-specific Immunoglobulin M in Dried Venous Blood Samples By Using a Commercial Enzyme Immunoassay." Journal of Clinical Microbiology, vol. 40, no. 1, 2002, pp. 5-9.
Riddell MA, Leydon JA, Catton MG, et al. Detection of measles virus-specific immunoglobulin M in dried venous blood samples by using a commercial enzyme immunoassay. J Clin Microbiol. 2002;40(1):5-9.
Riddell, M. A., Leydon, J. A., Catton, M. G., & Kelly, H. A. (2002). Detection of measles virus-specific immunoglobulin M in dried venous blood samples by using a commercial enzyme immunoassay. Journal of Clinical Microbiology, 40(1), 5-9.
Riddell MA, et al. Detection of Measles Virus-specific Immunoglobulin M in Dried Venous Blood Samples By Using a Commercial Enzyme Immunoassay. J Clin Microbiol. 2002;40(1):5-9. PubMed PMID: 11773084.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Detection of measles virus-specific immunoglobulin M in dried venous blood samples by using a commercial enzyme immunoassay. AU - Riddell,Michaela A, AU - Leydon,Jennie A, AU - Catton,Mike G, AU - Kelly,Heath A, PY - 2002/1/5/pubmed PY - 2002/2/13/medline PY - 2002/1/5/entrez SP - 5 EP - 9 JF - Journal of clinical microbiology JO - J Clin Microbiol VL - 40 IS - 1 N2 - The optical densities (ODs) of 216 dried venous blood (DVB) samples submitted to the Victorian Infectious Diseases Reference Laboratory as part of enhanced measles surveillance were compared to the ODs of the corresponding serum samples collected at the same time. DVB samples, stored for up to 24 months at 4 degrees C, were tested by the Dade Behring Enzygnost Anti-Measles-Virus/IgM immunoassay. Elution and testing conditions were optimized with the use of spiked DVB samples. The assay showed an overall sensitivity of 90.2% and a specificity of 98.8% for DVB samples compared to the results for serum. When the results were analyzed according to the length of time that the DVB sample had been stored, the assay was 100% sensitive and 97% specific according to the ODs for those samples stored for less than 6 months compared to the results for the corresponding serum samples, with 97.7% agreement between the results for the two sample types. These results demonstrate the potential for the use of DVB samples for the diagnosis of measles in routine diagnostic laboratories. SN - 0095-1137 UR - https://www.unboundmedicine.com/medline/citation/11773084/Detection_of_measles_virus_specific_immunoglobulin_M_in_dried_venous_blood_samples_by_using_a_commercial_enzyme_immunoassay_ L2 - https://journals.asm.org/doi/10.1128/JCM.40.1.5-9.2002?url_ver=Z39.88-2003&rfr_id=ori:rid:crossref.org&rfr_dat=cr_pub=pubmed DB - PRIME DP - Unbound Medicine ER -