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Dietary polyunsaturated fatty acids improve histological and biochemical alterations in rats with experimental ulcerative colitis.
J Nutr. 2002 Jan; 132(1):11-9.JN

Abstract

The aim of the present study was to determine whether dietary intake of monounsaturated (MUFA) and/or polyunsaturated fatty acids (PUFA) of the (n- 3) and (n-6) series could improve intestinal damage and reduce inflammation in experimental ulcerative colitis (UC). Rats were treated with 80 mg/kg body of 2,4,6-trinitrobenzenesulfonic acid and fed for 1 or 2 wk diets enriched in olive oil (OO), fish oil (FO), or purified pig brain phospholipids (BPL), as sources of monounsaturated and PUFA of the (n-3) and (n-3) + (n-6) series. Evaluation of macroscopic and microscopic colonic damage was assessed. Ultrastructural and histologic changes were analyzed as well as plasma and colonic mucosa fatty acid profiles and some biochemical markers of injury and inflammation [alkaline phosphatase (AP), mieloperoxidase (MPO), prostaglandin E(2) (PGE(2)) and leukotriene B(4)]. Fatty acid profiles of both plasma and mucosa mostly reflected the dietary fatty acid composition. Plasma MUFA proportions were higher in UC animals fed the OO diet compared with FO or BPL groups 1 and 2 wk and (n-3) long chain PUFA (LC-PUFA) were higher in the FO than in the OO and BPL groups. At 1 wk, UC led to lower MUFA mucosa levels and (n-3)LC-PUFA were higher in the FO group compared with the OO and BPL groups. Rats with UC fed FO at 1 wk showed significantly less macroscopic and microscopic colonic damage. They also have lower AP and MPO activities and PGE(2) levels compared with the OO and BPL groups and showed enhanced histological repair, less necrotic areas within the mucosa, and more goblet cells with mature mucin granules. These results suggest that the use of balanced diets containing (n-3) LC-PUFA could ameliorate the inflammation and mucosal damage in UC.

Authors+Show Affiliations

Department of Biochemistry and Molecular Biology, University of Granada, 18071 Granada, Spain.No affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

11773501

Citation

Nieto, Natalia, et al. "Dietary Polyunsaturated Fatty Acids Improve Histological and Biochemical Alterations in Rats With Experimental Ulcerative Colitis." The Journal of Nutrition, vol. 132, no. 1, 2002, pp. 11-9.
Nieto N, Torres MI, Ríos A, et al. Dietary polyunsaturated fatty acids improve histological and biochemical alterations in rats with experimental ulcerative colitis. J Nutr. 2002;132(1):11-9.
Nieto, N., Torres, M. I., Ríos, A., & Gil, A. (2002). Dietary polyunsaturated fatty acids improve histological and biochemical alterations in rats with experimental ulcerative colitis. The Journal of Nutrition, 132(1), 11-9.
Nieto N, et al. Dietary Polyunsaturated Fatty Acids Improve Histological and Biochemical Alterations in Rats With Experimental Ulcerative Colitis. J Nutr. 2002;132(1):11-9. PubMed PMID: 11773501.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Dietary polyunsaturated fatty acids improve histological and biochemical alterations in rats with experimental ulcerative colitis. AU - Nieto,Natalia, AU - Torres,María Isabel, AU - Ríos,Antonio, AU - Gil,Angel, PY - 2002/1/5/pubmed PY - 2002/2/6/medline PY - 2002/1/5/entrez SP - 11 EP - 9 JF - The Journal of nutrition JO - J. Nutr. VL - 132 IS - 1 N2 - The aim of the present study was to determine whether dietary intake of monounsaturated (MUFA) and/or polyunsaturated fatty acids (PUFA) of the (n- 3) and (n-6) series could improve intestinal damage and reduce inflammation in experimental ulcerative colitis (UC). Rats were treated with 80 mg/kg body of 2,4,6-trinitrobenzenesulfonic acid and fed for 1 or 2 wk diets enriched in olive oil (OO), fish oil (FO), or purified pig brain phospholipids (BPL), as sources of monounsaturated and PUFA of the (n-3) and (n-3) + (n-6) series. Evaluation of macroscopic and microscopic colonic damage was assessed. Ultrastructural and histologic changes were analyzed as well as plasma and colonic mucosa fatty acid profiles and some biochemical markers of injury and inflammation [alkaline phosphatase (AP), mieloperoxidase (MPO), prostaglandin E(2) (PGE(2)) and leukotriene B(4)]. Fatty acid profiles of both plasma and mucosa mostly reflected the dietary fatty acid composition. Plasma MUFA proportions were higher in UC animals fed the OO diet compared with FO or BPL groups 1 and 2 wk and (n-3) long chain PUFA (LC-PUFA) were higher in the FO than in the OO and BPL groups. At 1 wk, UC led to lower MUFA mucosa levels and (n-3)LC-PUFA were higher in the FO group compared with the OO and BPL groups. Rats with UC fed FO at 1 wk showed significantly less macroscopic and microscopic colonic damage. They also have lower AP and MPO activities and PGE(2) levels compared with the OO and BPL groups and showed enhanced histological repair, less necrotic areas within the mucosa, and more goblet cells with mature mucin granules. These results suggest that the use of balanced diets containing (n-3) LC-PUFA could ameliorate the inflammation and mucosal damage in UC. SN - 0022-3166 UR - https://www.unboundmedicine.com/medline/citation/11773501/Dietary_polyunsaturated_fatty_acids_improve_histological_and_biochemical_alterations_in_rats_with_experimental_ulcerative_colitis_ L2 - https://academic.oup.com/jn/article-lookup/doi/10.1093/jn/132.1.11 DB - PRIME DP - Unbound Medicine ER -