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Hyperbaric oxygen preconditioning induces neuroprotection against ischemia in transient not permanent middle cerebral artery occlusion rat model.
Chin Med J (Engl). 2000 Sep; 113(9):836-9.CM

Abstract

OBJECTIVE

This study was designed to determine if repeated hyperbaric oxygen (HBO) exposure induces ischemic tolerance in focal cerebral ischemia.

METHODS

Sixty male SD rats were used in this study. Thirty animals underwent transient middle cerebral artery occlusion (MCAO) and the other thirty permanent MCAO model. The rats were randomly allocated to 3 sub-groups: control group (n = 10), HBO-3 group (n = 10), and HBO-5 group (n = 10). The animals in HBO-3 and HBO-5 groups received 1 hour hyperbaric oxygenation at 2.5 atmosphere absolute (ATA) in 100% oxygen every day for 3 and 5 days, respectively. The animals in the control group received sham treatments. 24 hours after the last HBO, transient MCAO (120 min) and permanent MCAO were induced by introducing a 3-0 nylon monofilament suture through internal carotid artery based on the Koizumi technique. The neurological outcome was evaluated until 24 hours after reperfusion in transient MCAO rats and ischemia in permanent MCAO rats. The infarct volume was then assessed by TTC staining.

RESULTS

In transient MCAO rats, the neurological outcome in both the HBO-3 and HBO-5 groups was better than that of the control group (P < 0.05 and 0.001). The infarct volume decreased from 171.5 +/- 113 mm3 to 40.6 +/- 49.9 mm3(P < 0.05) in the HBO-3 group and 16.2 +/- 28.8 mm3(P < 0.01) in the HBO-5 group. There were no significant differences in neurological outcome and infarct volume among the three groups in permanent MCAO rats.

CONCLUSIONS

The present study demonstrated that HBO preconditioning can induce ischemic tolerance in transient not permanent MCAO rats in a "dose-dependent" manner.

Authors+Show Affiliations

Department of Anesthesiology, Xijing Hospital of the Fourth Military Medical University, Xi'an 710032, China.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

11776082

Citation

Xiong, L, et al. "Hyperbaric Oxygen Preconditioning Induces Neuroprotection Against Ischemia in Transient Not Permanent Middle Cerebral Artery Occlusion Rat Model." Chinese Medical Journal, vol. 113, no. 9, 2000, pp. 836-9.
Xiong L, Zhu Z, Dong H, et al. Hyperbaric oxygen preconditioning induces neuroprotection against ischemia in transient not permanent middle cerebral artery occlusion rat model. Chin Med J. 2000;113(9):836-9.
Xiong, L., Zhu, Z., Dong, H., Hu, W., Hou, L., & Chen, S. (2000). Hyperbaric oxygen preconditioning induces neuroprotection against ischemia in transient not permanent middle cerebral artery occlusion rat model. Chinese Medical Journal, 113(9), 836-9.
Xiong L, et al. Hyperbaric Oxygen Preconditioning Induces Neuroprotection Against Ischemia in Transient Not Permanent Middle Cerebral Artery Occlusion Rat Model. Chin Med J. 2000;113(9):836-9. PubMed PMID: 11776082.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Hyperbaric oxygen preconditioning induces neuroprotection against ischemia in transient not permanent middle cerebral artery occlusion rat model. AU - Xiong,L, AU - Zhu,Z, AU - Dong,H, AU - Hu,W, AU - Hou,L, AU - Chen,S, PY - 2002/1/5/pubmed PY - 2002/2/6/medline PY - 2002/1/5/entrez SP - 836 EP - 9 JF - Chinese medical journal JO - Chin. Med. J. VL - 113 IS - 9 N2 - OBJECTIVE: This study was designed to determine if repeated hyperbaric oxygen (HBO) exposure induces ischemic tolerance in focal cerebral ischemia. METHODS: Sixty male SD rats were used in this study. Thirty animals underwent transient middle cerebral artery occlusion (MCAO) and the other thirty permanent MCAO model. The rats were randomly allocated to 3 sub-groups: control group (n = 10), HBO-3 group (n = 10), and HBO-5 group (n = 10). The animals in HBO-3 and HBO-5 groups received 1 hour hyperbaric oxygenation at 2.5 atmosphere absolute (ATA) in 100% oxygen every day for 3 and 5 days, respectively. The animals in the control group received sham treatments. 24 hours after the last HBO, transient MCAO (120 min) and permanent MCAO were induced by introducing a 3-0 nylon monofilament suture through internal carotid artery based on the Koizumi technique. The neurological outcome was evaluated until 24 hours after reperfusion in transient MCAO rats and ischemia in permanent MCAO rats. The infarct volume was then assessed by TTC staining. RESULTS: In transient MCAO rats, the neurological outcome in both the HBO-3 and HBO-5 groups was better than that of the control group (P < 0.05 and 0.001). The infarct volume decreased from 171.5 +/- 113 mm3 to 40.6 +/- 49.9 mm3(P < 0.05) in the HBO-3 group and 16.2 +/- 28.8 mm3(P < 0.01) in the HBO-5 group. There were no significant differences in neurological outcome and infarct volume among the three groups in permanent MCAO rats. CONCLUSIONS: The present study demonstrated that HBO preconditioning can induce ischemic tolerance in transient not permanent MCAO rats in a "dose-dependent" manner. SN - 0366-6999 UR - https://www.unboundmedicine.com/medline/citation/11776082/Hyperbaric_oxygen_preconditioning_induces_neuroprotection_against_ischemia_in_transient_not_permanent_middle_cerebral_artery_occlusion_rat_model_ DB - PRIME DP - Unbound Medicine ER -