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[Study on the anti-müllerian hormone served as a marker for granulosa cell tumor of ovary].
Zhonghua Fu Chan Ke Za Zhi 2000; 35(6):356-8ZF

Abstract

OBJECTIVE

To probe the experimental basis for anti-mullerian hormone(AMH) served as a specific tumor label in diagnosis of granulosa cell tumor(GCT) of ovary.

METHODS

The expression of AMH and its receptor mRNA in early and late GCT of ovary in transgenic mice was studied by using immunohistochemistry, in stiu hybridization and Northern hybridization. Furthermore, the expression of AMH and its receptor mRNA in cultured GCT cells was also determined by using radioiodine labeling method and Northern hybridization.

RESULTS

In the early ovary GCT of transgenic mice, immunohistochemistry showed that AMH was positively stained as brownish yellow granulars, which were located in the cell membrane of the tumor, in situ hybridization showed its receptor mRNA was positive as blue granulars, which were also located in the cell membrane. The positive rate of both AMH and its receptor mRNA was 100%. Results by northern hybridization also showed that the positive rate of AMH and its receptor mRNA in the early ovary GCT and GCT cells was 100%. Radioautography indicated that there was a radioactive bright point, the binding rate of 125I labeled AMH with tumor cells reached 100%.

CONCLUSIONS

AMH can be served as a specific tumor marker in the diagnosis of difficult and complicated tumors of ovary, and in the diagnosis, treatment and follow-up survey of the early GCT.

Authors+Show Affiliations

Department of Obstetrics and Gynecology, RuiJin Hospital, Shanghai Second Medical University, Shanghai 200025, China.No affiliation info availableNo affiliation info available

Pub Type(s)

English Abstract
Journal Article

Language

chi

PubMed ID

11776178

Citation

Long, W, et al. "[Study On the Anti-müllerian Hormone Served as a Marker for Granulosa Cell Tumor of Ovary]." Zhonghua Fu Chan Ke Za Zhi, vol. 35, no. 6, 2000, pp. 356-8.
Long W, Wang W, Rey R. [Study on the anti-müllerian hormone served as a marker for granulosa cell tumor of ovary]. Zhonghua Fu Chan Ke Za Zhi. 2000;35(6):356-8.
Long, W., Wang, W., & Rey, R. (2000). [Study on the anti-müllerian hormone served as a marker for granulosa cell tumor of ovary]. Zhonghua Fu Chan Ke Za Zhi, 35(6), pp. 356-8.
Long W, Wang W, Rey R. [Study On the Anti-müllerian Hormone Served as a Marker for Granulosa Cell Tumor of Ovary]. Zhonghua Fu Chan Ke Za Zhi. 2000;35(6):356-8. PubMed PMID: 11776178.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - [Study on the anti-müllerian hormone served as a marker for granulosa cell tumor of ovary]. AU - Long,W, AU - Wang,W, AU - Rey,R, PY - 2002/1/5/pubmed PY - 2002/1/31/medline PY - 2002/1/5/entrez SP - 356 EP - 8 JF - Zhonghua fu chan ke za zhi JO - Zhonghua Fu Chan Ke Za Zhi VL - 35 IS - 6 N2 - OBJECTIVE: To probe the experimental basis for anti-mullerian hormone(AMH) served as a specific tumor label in diagnosis of granulosa cell tumor(GCT) of ovary. METHODS: The expression of AMH and its receptor mRNA in early and late GCT of ovary in transgenic mice was studied by using immunohistochemistry, in stiu hybridization and Northern hybridization. Furthermore, the expression of AMH and its receptor mRNA in cultured GCT cells was also determined by using radioiodine labeling method and Northern hybridization. RESULTS: In the early ovary GCT of transgenic mice, immunohistochemistry showed that AMH was positively stained as brownish yellow granulars, which were located in the cell membrane of the tumor, in situ hybridization showed its receptor mRNA was positive as blue granulars, which were also located in the cell membrane. The positive rate of both AMH and its receptor mRNA was 100%. Results by northern hybridization also showed that the positive rate of AMH and its receptor mRNA in the early ovary GCT and GCT cells was 100%. Radioautography indicated that there was a radioactive bright point, the binding rate of 125I labeled AMH with tumor cells reached 100%. CONCLUSIONS: AMH can be served as a specific tumor marker in the diagnosis of difficult and complicated tumors of ovary, and in the diagnosis, treatment and follow-up survey of the early GCT. SN - 0529-567X UR - https://www.unboundmedicine.com/medline/citation/11776178/[Study_on_the_anti_müllerian_hormone_served_as_a_marker_for_granulosa_cell_tumor_of_ovary]_ L2 - https://medlineplus.gov/ovariancancer.html DB - PRIME DP - Unbound Medicine ER -