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Dual effects of sulfated D-galactans from the red algae Botryocladia occidentalis preventing thrombosis and inducing platelet aggregation.
Thromb Haemost. 2001 Dec; 86(6):1540-6.TH

Abstract

Sulfated D-galactans occur on the red algae Botryocladia occidentalis as three fractions that differ in their sulfate content. Fractions F2 and F3 are potent anticoagulants. Like heparin, they enhance thrombin and factor Xa inhibition by antithrombin and/or heparin cofactor II. The inhibition potency increases simultaneously with the sulfate content of the fractions. The antithrombotic activity of these sulfated D-galactans was investigated on an experimental thrombosis model in which thrombus formation was induced by a combination of stasis and hypercoagulability. In contrast with heparin. the sulfated D-galactans showed a dual dose-response curve preventing thrombosis at doses up to approximately 0.5 mg/ kg body weight but losing the effect at higher doses. This unexpected behavior is probably due to a combined action of the sulfated D-galactan as anticoagulant and also as a strong inducer of platelet aggregation. In platelet-depleted animals the antithrombotic activity at higher dose of sulfated D-galactan is restored and almost total inhibition of thrombus formation is achieved. The sulfated D-galactan has no hemorrhagic effect even at high doses, possibly as a consequence of its effect on platelet aggregation. At comparable dose heparin has an intense bleeding effect. These results indicate that new polysaccharides, with well-defined structures, can help to distinguish events, such as antithrombotic and anticoagulant activities, bleeding and platelet-aggregating effects, which are obscure when induced simultaneously by a single compound.

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Authors+Show Affiliations

Farias WR
Hospital Universitário Clementino Fraga Filho and Departamento de Bioquímica Médica, Centro de Ciências da Saúde, Universidade Federal do Rio de Janeiro, RJ, Brazil.
Nazareth RA
No affiliation info available
Mourão PA
No affiliation info available

MeSH

AnimalsAnticoagulantsBrazilDose-Response Relationship, DrugDrug Evaluation, PreclinicalFemaleFibrinolytic AgentsGalactansHemorrhageHeparinMalePartial Thromboplastin TimePhytotherapyPlant ExtractsPlatelet AggregationRatsRats, WistarRhodophytaSulfatesThromboplastinVena Cava, InferiorVenous Thrombosis

Pub Type(s)

Comparative Study
Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

11776325

Citation

Farias, W R., et al. "Dual Effects of Sulfated D-galactans From the Red Algae Botryocladia Occidentalis Preventing Thrombosis and Inducing Platelet Aggregation." Thrombosis and Haemostasis, vol. 86, no. 6, 2001, pp. 1540-6.
Farias WR, Nazareth RA, Mourão PA. Dual effects of sulfated D-galactans from the red algae Botryocladia occidentalis preventing thrombosis and inducing platelet aggregation. Thromb Haemost. 2001;86(6):1540-6.
Farias, W. R., Nazareth, R. A., & Mourão, P. A. (2001). Dual effects of sulfated D-galactans from the red algae Botryocladia occidentalis preventing thrombosis and inducing platelet aggregation. Thrombosis and Haemostasis, 86(6), 1540-6.
Farias WR, Nazareth RA, Mourão PA. Dual Effects of Sulfated D-galactans From the Red Algae Botryocladia Occidentalis Preventing Thrombosis and Inducing Platelet Aggregation. Thromb Haemost. 2001;86(6):1540-6. PubMed PMID: 11776325.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Dual effects of sulfated D-galactans from the red algae Botryocladia occidentalis preventing thrombosis and inducing platelet aggregation. AU - Farias,W R, AU - Nazareth,R A, AU - Mourão,P A, PY - 2002/1/5/pubmed PY - 2002/7/13/medline PY - 2002/1/5/entrez SP - 1540 EP - 6 JF - Thrombosis and haemostasis JO - Thromb Haemost VL - 86 IS - 6 N2 - Sulfated D-galactans occur on the red algae Botryocladia occidentalis as three fractions that differ in their sulfate content. Fractions F2 and F3 are potent anticoagulants. Like heparin, they enhance thrombin and factor Xa inhibition by antithrombin and/or heparin cofactor II. The inhibition potency increases simultaneously with the sulfate content of the fractions. The antithrombotic activity of these sulfated D-galactans was investigated on an experimental thrombosis model in which thrombus formation was induced by a combination of stasis and hypercoagulability. In contrast with heparin. the sulfated D-galactans showed a dual dose-response curve preventing thrombosis at doses up to approximately 0.5 mg/ kg body weight but losing the effect at higher doses. This unexpected behavior is probably due to a combined action of the sulfated D-galactan as anticoagulant and also as a strong inducer of platelet aggregation. In platelet-depleted animals the antithrombotic activity at higher dose of sulfated D-galactan is restored and almost total inhibition of thrombus formation is achieved. The sulfated D-galactan has no hemorrhagic effect even at high doses, possibly as a consequence of its effect on platelet aggregation. At comparable dose heparin has an intense bleeding effect. These results indicate that new polysaccharides, with well-defined structures, can help to distinguish events, such as antithrombotic and anticoagulant activities, bleeding and platelet-aggregating effects, which are obscure when induced simultaneously by a single compound. SN - 0340-6245 UR - https://www.unboundmedicine.com/medline/citation/11776325/Dual_effects_of_sulfated_D_galactans_from_the_red_algae_Botryocladia_occidentalis_preventing_thrombosis_and_inducing_platelet_aggregation_ L2 - http://ovidsp.ovid.com/ovidweb.cgi?T=JS&PAGE=linkout&SEARCH=11776325.ui DB - PRIME DP - Unbound Medicine ER -