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Lack of correlation of vacA genotype, cagA gene of Helicobacter pylori and their expression products with various gastroduodenal diseases.
Chin Med J (Engl). 2001 Jul; 114(7):703-6.CM

Abstract

OBJECTIVE

To investigate the correlation among vacA genotypes, cagA gene, VacA, serum CagA antibodies of Helicobacter pylori (H. pylori) and gastroduodenal diseases.

METHODS

vacA genotypes and cagA gene of 62 H. pylori strains isolated from patients with chronic gastritis, peptic ulcer and gastric cancer were tested by polymerase chain reaction, and Hela cell assay for VacA activity in vitro. Serum CagA antibodies were measured by EIA method in the same patients.

RESULTS

All 62 H. pylori strains possessed the vacA gene and vacA genotypes of all strains were type s1a/m2. Total positive rate of cagA gene was 56.45%; the positive rates of cagA gene of H. pylori strains isolated from patients with chronic gastritis, peptic ulcer and gastric cancer were 55.56%, 54.17% and 63.64%, respectively (P > 0.05). The total positive rate of VacA was 37.10%; the positive rates of VacA produced by H. pylori strains isolated from patients with chronic gastritis, peptic ulcer and gastric cancer were 33.33%, 29.17% and 63.64%, respectively (P > 0.05). The positive rates of CagA antibodies in patients with chronic gastritis, peptic ulcer and gastric cancer were 70.37%, 79.17% and 40.00%, respectively (P > 0.05). The total positive rate of CagA antibodies was 68.85%.

CONCLUSION

There was no correlation among cagA gene and vacA genotypes of H. pylori, VacA, serum CagA antibodies and various gastroduodenal diseases.

Authors+Show Affiliations

Department of Gastroenterology, Affiliated Hospital of Zhenjiang Medical College, Zhenjiang 212001, Jiangsu Province, China. youli@public.zj.js.cnNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article

Language

eng

PubMed ID

11780331

Citation

Zhang, Y, et al. "Lack of Correlation of vacA Genotype, cagA Gene of Helicobacter Pylori and Their Expression Products With Various Gastroduodenal Diseases." Chinese Medical Journal, vol. 114, no. 7, 2001, pp. 703-6.
Zhang Y, Liu H, Zhou K. Lack of correlation of vacA genotype, cagA gene of Helicobacter pylori and their expression products with various gastroduodenal diseases. Chin Med J (Engl). 2001;114(7):703-6.
Zhang, Y., Liu, H., & Zhou, K. (2001). Lack of correlation of vacA genotype, cagA gene of Helicobacter pylori and their expression products with various gastroduodenal diseases. Chinese Medical Journal, 114(7), 703-6.
Zhang Y, Liu H, Zhou K. Lack of Correlation of vacA Genotype, cagA Gene of Helicobacter Pylori and Their Expression Products With Various Gastroduodenal Diseases. Chin Med J (Engl). 2001;114(7):703-6. PubMed PMID: 11780331.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Lack of correlation of vacA genotype, cagA gene of Helicobacter pylori and their expression products with various gastroduodenal diseases. AU - Zhang,Y, AU - Liu,H, AU - Zhou,K, PY - 2002/1/10/pubmed PY - 2002/2/22/medline PY - 2002/1/10/entrez SP - 703 EP - 6 JF - Chinese medical journal JO - Chin Med J (Engl) VL - 114 IS - 7 N2 - OBJECTIVE: To investigate the correlation among vacA genotypes, cagA gene, VacA, serum CagA antibodies of Helicobacter pylori (H. pylori) and gastroduodenal diseases. METHODS: vacA genotypes and cagA gene of 62 H. pylori strains isolated from patients with chronic gastritis, peptic ulcer and gastric cancer were tested by polymerase chain reaction, and Hela cell assay for VacA activity in vitro. Serum CagA antibodies were measured by EIA method in the same patients. RESULTS: All 62 H. pylori strains possessed the vacA gene and vacA genotypes of all strains were type s1a/m2. Total positive rate of cagA gene was 56.45%; the positive rates of cagA gene of H. pylori strains isolated from patients with chronic gastritis, peptic ulcer and gastric cancer were 55.56%, 54.17% and 63.64%, respectively (P > 0.05). The total positive rate of VacA was 37.10%; the positive rates of VacA produced by H. pylori strains isolated from patients with chronic gastritis, peptic ulcer and gastric cancer were 33.33%, 29.17% and 63.64%, respectively (P > 0.05). The positive rates of CagA antibodies in patients with chronic gastritis, peptic ulcer and gastric cancer were 70.37%, 79.17% and 40.00%, respectively (P > 0.05). The total positive rate of CagA antibodies was 68.85%. CONCLUSION: There was no correlation among cagA gene and vacA genotypes of H. pylori, VacA, serum CagA antibodies and various gastroduodenal diseases. SN - 0366-6999 UR - https://www.unboundmedicine.com/medline/citation/11780331/Lack_of_correlation_of_vacA_genotype_cagA_gene_of_Helicobacter_pylori_and_their_expression_products_with_various_gastroduodenal_diseases_ L2 - https://medlineplus.gov/pepticulcer.html DB - PRIME DP - Unbound Medicine ER -