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Gene expression of glucose transporters and its regulation by glucose in mesothelial cells.
Chin Med J (Engl) 2001; 114(5):477-80CM

Abstract

OBJECTIVE

To observe the influence of glucose on the expression of glucose transporters (GluTs) in peritoneal tissues.

METHODS

Mesothelial cells (MsCs) from Sprague-Dawley (SD) rats were cultured in medium with glucose 214.4 mmol/L or 75.5 mmol/L. The normal medium with glucose 17.5 mmol/L was used as control. Total RNA was extracted from each sample after 24 hours incubation. Reverse transcript polymerase chain reaction (RT-PCR) was performed with primers corresponding to sodium-glucose transporter (SGIT1) and GluT1-GluT4. mRNA expression of the above GluTs from each sample was measured with quantitative PCR.

RESULTS

GluT1 and GluT2 mRNA can be detected in MsCs from SD rats, while no positive bands can be found specifically for GluT3, GluT4 and SGiT1. Quantitating the amount of PCR products indicated that the levels of GluT1 mRNA in MsCs cultured 24 h in both 214.4 mmol/L glucose and 75.5 mmol/L glucose medium decreased dramatically compared with that in normal medium (P < or = 0.01). While under the same conditions, the levels of GluT2 mRNA in MsCs cultured 24 h in 214.4 mmol/L and 75.5 mmol/L glucose medium both increased significantly (P < 0.01).

CONCLUSIONS

GluT1 is strongly expressed in MsCs under normal glucose levels and decreased dramatically under high glucose conditions, while GluT2 expressed at a low level in normal medium and increased greatly after incubation in high glucose conditions. This may play a great role in glucose absorption during peritoneal dialysis and have some connection with ultrafiltration failure due to the alteration of glucose absorption after long-term dialysis.

Authors+Show Affiliations

Renal Department, First Affiliated Hospital of Sun Yat-Sen University of Medical Sciences, Guangzhou 510089, China.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

11780407

Citation

Lu, C, et al. "Gene Expression of Glucose Transporters and Its Regulation By Glucose in Mesothelial Cells." Chinese Medical Journal, vol. 114, no. 5, 2001, pp. 477-80.
Lu C, Ye R, Yang Q, et al. Gene expression of glucose transporters and its regulation by glucose in mesothelial cells. Chin Med J. 2001;114(5):477-80.
Lu, C., Ye, R., Yang, Q., Yang, X., Li, H., & Li, Y. (2001). Gene expression of glucose transporters and its regulation by glucose in mesothelial cells. Chinese Medical Journal, 114(5), pp. 477-80.
Lu C, et al. Gene Expression of Glucose Transporters and Its Regulation By Glucose in Mesothelial Cells. Chin Med J. 2001;114(5):477-80. PubMed PMID: 11780407.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Gene expression of glucose transporters and its regulation by glucose in mesothelial cells. AU - Lu,C, AU - Ye,R, AU - Yang,Q, AU - Yang,X, AU - Li,H, AU - Li,Y, PY - 2002/1/10/pubmed PY - 2002/2/22/medline PY - 2002/1/10/entrez SP - 477 EP - 80 JF - Chinese medical journal JO - Chin. Med. J. VL - 114 IS - 5 N2 - OBJECTIVE: To observe the influence of glucose on the expression of glucose transporters (GluTs) in peritoneal tissues. METHODS: Mesothelial cells (MsCs) from Sprague-Dawley (SD) rats were cultured in medium with glucose 214.4 mmol/L or 75.5 mmol/L. The normal medium with glucose 17.5 mmol/L was used as control. Total RNA was extracted from each sample after 24 hours incubation. Reverse transcript polymerase chain reaction (RT-PCR) was performed with primers corresponding to sodium-glucose transporter (SGIT1) and GluT1-GluT4. mRNA expression of the above GluTs from each sample was measured with quantitative PCR. RESULTS: GluT1 and GluT2 mRNA can be detected in MsCs from SD rats, while no positive bands can be found specifically for GluT3, GluT4 and SGiT1. Quantitating the amount of PCR products indicated that the levels of GluT1 mRNA in MsCs cultured 24 h in both 214.4 mmol/L glucose and 75.5 mmol/L glucose medium decreased dramatically compared with that in normal medium (P < or = 0.01). While under the same conditions, the levels of GluT2 mRNA in MsCs cultured 24 h in 214.4 mmol/L and 75.5 mmol/L glucose medium both increased significantly (P < 0.01). CONCLUSIONS: GluT1 is strongly expressed in MsCs under normal glucose levels and decreased dramatically under high glucose conditions, while GluT2 expressed at a low level in normal medium and increased greatly after incubation in high glucose conditions. This may play a great role in glucose absorption during peritoneal dialysis and have some connection with ultrafiltration failure due to the alteration of glucose absorption after long-term dialysis. SN - 0366-6999 UR - https://www.unboundmedicine.com/medline/citation/11780407/Gene_expression_of_glucose_transporters_and_its_regulation_by_glucose_in_mesothelial_cells_ DB - PRIME DP - Unbound Medicine ER -