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Immunotoxin depletion of T cells and its effect on hematopoietic progenitor cells in human cord blood.
Chin Med J (Engl). 2001 Apr; 114(4):355-9.CM

Abstract

OBJECTIVE

To study the selective toxicity of immunotoxin (IT) on T cells in cord blood and simultaneously determine its effect on hematopoietic progenitor cells.

METHODS

The percentage of CD5 and CD8 T cell subsets in cord blood (CB) and bone marrow (BM) as well as peripheral blood (PB) was measured by immunoenzymatic labeling of monoclonal antibodies using immune complexes of alkaline phosphatase and monoclonal anti-alkaline phosphatase (APAAP complexes). One-way mixed lymphocyte cultures (MLC) were performed to compare the proliferative response of CB with that of PB. The proliferative capability of cord blood T cells and T lymphocyte transformation capacity were evaluated in the presence of anti-CD8 or anti-CD5 immunotoxin by one-way MLC and colorimetric MTT (tetrazolium) assay, respectively. The effect of IT on the growth of hematopoietic progenitor cell of colony forming unit-granulocyte and macrophage (CFU-GM), burst forming unit-erythroid(BFU-E), multipotential hemotapoietic progenitors (CFU-Mix) from CB were estimated by colony-forming assays.

RESULTS

A certain proportion of CD5 and CD8 T cells existed in CB. The alloproliferative capacity of CB was similar to that of PB. CD5: Ricin at a dosage of 1 x 10(-10)-1 x 10(-8) mmol/L and CD8: Ricin concentration in the range of 1 x 10(-9)-1 x 10(-8) mmol/L effectively decreased both the proliferative capability of T cells in MLC during CB and T cell transformation. Over the dosage of 1 x 10(-10)-1 x 10(-9) mmol/L, both kinds of IT didn't obviously affect the growth of hematopoietic progenitor cells.

CONCLUSION

CD5: Ricin and CD8: Ricin may effectively deplete T cells and may not significantly inhibit the function of hemaptopoietic cells at a specific dosage.

Authors+Show Affiliations

Department of Pediatrics, General Hospital of Guangzhou Military Area, Guangzhou 510010, China.No affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

11780453

Citation

Xu, M, et al. "Immunotoxin Depletion of T Cells and Its Effect On Hematopoietic Progenitor Cells in Human Cord Blood." Chinese Medical Journal, vol. 114, no. 4, 2001, pp. 355-9.
Xu M, Lu S, Shen B, et al. Immunotoxin depletion of T cells and its effect on hematopoietic progenitor cells in human cord blood. Chin Med J (Engl). 2001;114(4):355-9.
Xu, M., Lu, S., Shen, B., & Li, Y. (2001). Immunotoxin depletion of T cells and its effect on hematopoietic progenitor cells in human cord blood. Chinese Medical Journal, 114(4), 355-9.
Xu M, et al. Immunotoxin Depletion of T Cells and Its Effect On Hematopoietic Progenitor Cells in Human Cord Blood. Chin Med J (Engl). 2001;114(4):355-9. PubMed PMID: 11780453.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Immunotoxin depletion of T cells and its effect on hematopoietic progenitor cells in human cord blood. AU - Xu,M, AU - Lu,S, AU - Shen,B, AU - Li,Y, PY - 2002/1/10/pubmed PY - 2002/2/22/medline PY - 2002/1/10/entrez SP - 355 EP - 9 JF - Chinese medical journal JO - Chin Med J (Engl) VL - 114 IS - 4 N2 - OBJECTIVE: To study the selective toxicity of immunotoxin (IT) on T cells in cord blood and simultaneously determine its effect on hematopoietic progenitor cells. METHODS: The percentage of CD5 and CD8 T cell subsets in cord blood (CB) and bone marrow (BM) as well as peripheral blood (PB) was measured by immunoenzymatic labeling of monoclonal antibodies using immune complexes of alkaline phosphatase and monoclonal anti-alkaline phosphatase (APAAP complexes). One-way mixed lymphocyte cultures (MLC) were performed to compare the proliferative response of CB with that of PB. The proliferative capability of cord blood T cells and T lymphocyte transformation capacity were evaluated in the presence of anti-CD8 or anti-CD5 immunotoxin by one-way MLC and colorimetric MTT (tetrazolium) assay, respectively. The effect of IT on the growth of hematopoietic progenitor cell of colony forming unit-granulocyte and macrophage (CFU-GM), burst forming unit-erythroid(BFU-E), multipotential hemotapoietic progenitors (CFU-Mix) from CB were estimated by colony-forming assays. RESULTS: A certain proportion of CD5 and CD8 T cells existed in CB. The alloproliferative capacity of CB was similar to that of PB. CD5: Ricin at a dosage of 1 x 10(-10)-1 x 10(-8) mmol/L and CD8: Ricin concentration in the range of 1 x 10(-9)-1 x 10(-8) mmol/L effectively decreased both the proliferative capability of T cells in MLC during CB and T cell transformation. Over the dosage of 1 x 10(-10)-1 x 10(-9) mmol/L, both kinds of IT didn't obviously affect the growth of hematopoietic progenitor cells. CONCLUSION: CD5: Ricin and CD8: Ricin may effectively deplete T cells and may not significantly inhibit the function of hemaptopoietic cells at a specific dosage. SN - 0366-6999 UR - https://www.unboundmedicine.com/medline/citation/11780453/Immunotoxin_depletion_of_T_cells_and_its_effect_on_hematopoietic_progenitor_cells_in_human_cord_blood_ L2 - https://medlineplus.gov/stemcells.html DB - PRIME DP - Unbound Medicine ER -