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[Alterations in DPC4 gene in pancreatic cancers].
Zhonghua Zhong Liu Za Zhi 2001; 23(4):293-5ZZ

Abstract

OBJECTIVE

To examine the alterations in the DPC4 gene in pancreatic cancers.

METHODS

Five pancreatic adenocarcinoma cell lines (P1, P2, P3, P4, and P7) and 11 fresh frozen pancreatic cancer tissues were monitored with polymerase chain reaction (PCR), single-strand conformation polymorphism (SSCP) analysis to demonstrate the sequence of deletion and mutation in 6 exons (1, 2, 3, 4, 8, and 11) of the DPC4 gene.

RESULTS

Mutation in the DPC4 gene was found in 3 of 5 cells lines (P1, P2, and P3). Three of eleven fresh-frozen tissues showed homozygous deletions and two showed intragenic mutations, with a gene alteration frequency of 45.5% (5/11).

CONCLUSION

Alterations in tumor-suppressor gene DPC4 may play an important role during the tumorigenesis of pancreatic cancer.

Authors+Show Affiliations

Peking Union Medical College Hospital, Chinese Academy of Medical Sciences, Beijing 100730, China.No affiliation info availableNo affiliation info available

Pub Type(s)

English Abstract
Journal Article

Language

chi

PubMed ID

11783110

Citation

Gu, L, et al. "[Alterations in DPC4 Gene in Pancreatic Cancers]." Zhonghua Zhong Liu Za Zhi [Chinese Journal of Oncology], vol. 23, no. 4, 2001, pp. 293-5.
Gu L, Chen J, Cui Q. [Alterations in DPC4 gene in pancreatic cancers]. Zhonghua Zhong Liu Za Zhi. 2001;23(4):293-5.
Gu, L., Chen, J., & Cui, Q. (2001). [Alterations in DPC4 gene in pancreatic cancers]. Zhonghua Zhong Liu Za Zhi [Chinese Journal of Oncology], 23(4), pp. 293-5.
Gu L, Chen J, Cui Q. [Alterations in DPC4 Gene in Pancreatic Cancers]. Zhonghua Zhong Liu Za Zhi. 2001;23(4):293-5. PubMed PMID: 11783110.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - [Alterations in DPC4 gene in pancreatic cancers]. AU - Gu,L, AU - Chen,J, AU - Cui,Q, PY - 2002/1/11/pubmed PY - 2002/3/2/medline PY - 2002/1/11/entrez SP - 293 EP - 5 JF - Zhonghua zhong liu za zhi [Chinese journal of oncology] JO - Zhonghua Zhong Liu Za Zhi VL - 23 IS - 4 N2 - OBJECTIVE: To examine the alterations in the DPC4 gene in pancreatic cancers. METHODS: Five pancreatic adenocarcinoma cell lines (P1, P2, P3, P4, and P7) and 11 fresh frozen pancreatic cancer tissues were monitored with polymerase chain reaction (PCR), single-strand conformation polymorphism (SSCP) analysis to demonstrate the sequence of deletion and mutation in 6 exons (1, 2, 3, 4, 8, and 11) of the DPC4 gene. RESULTS: Mutation in the DPC4 gene was found in 3 of 5 cells lines (P1, P2, and P3). Three of eleven fresh-frozen tissues showed homozygous deletions and two showed intragenic mutations, with a gene alteration frequency of 45.5% (5/11). CONCLUSION: Alterations in tumor-suppressor gene DPC4 may play an important role during the tumorigenesis of pancreatic cancer. SN - 0253-3766 UR - https://www.unboundmedicine.com/medline/citation/11783110/[Alterations_in_DPC4_gene_in_pancreatic_cancers]_ L2 - https://biocyc.org/gene?orgid=HUMAN&id=HS06859 DB - PRIME DP - Unbound Medicine ER -