[Alterations in DPC4 gene in pancreatic cancers].Zhonghua Zhong Liu Za Zhi 2001; 23(4):293-5ZZ
To examine the alterations in the DPC4 gene in pancreatic cancers.
Five pancreatic adenocarcinoma cell lines (P1, P2, P3, P4, and P7) and 11 fresh frozen pancreatic cancer tissues were monitored with polymerase chain reaction (PCR), single-strand conformation polymorphism (SSCP) analysis to demonstrate the sequence of deletion and mutation in 6 exons (1, 2, 3, 4, 8, and 11) of the DPC4 gene.
Mutation in the DPC4 gene was found in 3 of 5 cells lines (P1, P2, and P3). Three of eleven fresh-frozen tissues showed homozygous deletions and two showed intragenic mutations, with a gene alteration frequency of 45.5% (5/11).
Alterations in tumor-suppressor gene DPC4 may play an important role during the tumorigenesis of pancreatic cancer.