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Genetic profile of 22 pancreatic carcinoma cell lines. Analysis of K-ras, p53, p16 and DPC4/Smad4.

Abstract

The K-ras, p53, p16 and DPC4 genes are among those most frequently altered in pancreatic ductal carcinoma. We analyzed 22 cell lines for alterations in these genes by direct sequence analysis and methylation-specific polymerase chain reaction. These cell lines showed mutations in K-ras and p53 at frequencies of 91% and 95%, respectively. Alterations in p16INK4a were found in all cases and included nine homozygous deletions, seven mutations and promoter methylation in six cases. Eight cell lines (36%) had an alteration of DPC4, including one mutation and seven homozygous deletions. The most typical mutational profile involved K-ras, p53, and p16INK4a, concurrently aberrated in 20 cases (91%). Eight cell lines had alterations in all four genes. Inactivation of DPC4 was always accompanied by alteration of all of the other three genes. This comprehensive data regarding the cumulative genetic alterations in pancreatic carcinoma cell lines will be of great value for studies involving drug sensitivity or resistance that may be associated with inactivation of a particular gene or molecular pathway.

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  • Authors+Show Affiliations

    ,

    Department of Pathology, University of Verona, Italy.

    , , , , , , , , , , ,

    Source

    MeSH

    Carcinoma, Pancreatic Ductal
    DNA Mutational Analysis
    DNA, Neoplasm
    DNA-Binding Proteins
    Genes, p16
    Genes, p53
    Genes, ras
    Humans
    Mutation
    Pancreatic Neoplasms
    Polymerase Chain Reaction
    Smad4 Protein
    Trans-Activators
    Tumor Cells, Cultured

    Pub Type(s)

    Journal Article
    Research Support, Non-U.S. Gov't

    Language

    eng

    PubMed ID

    11787853

    Citation

    Moore, P S., et al. "Genetic Profile of 22 Pancreatic Carcinoma Cell Lines. Analysis of K-ras, P53, P16 and DPC4/Smad4." Virchows Archiv : an International Journal of Pathology, vol. 439, no. 6, 2001, pp. 798-802.
    Moore PS, Sipos B, Orlandini S, et al. Genetic profile of 22 pancreatic carcinoma cell lines. Analysis of K-ras, p53, p16 and DPC4/Smad4. Virchows Arch. 2001;439(6):798-802.
    Moore, P. S., Sipos, B., Orlandini, S., Sorio, C., Real, F. X., Lemoine, N. R., ... Scarpa, A. (2001). Genetic profile of 22 pancreatic carcinoma cell lines. Analysis of K-ras, p53, p16 and DPC4/Smad4. Virchows Archiv : an International Journal of Pathology, 439(6), pp. 798-802.
    Moore PS, et al. Genetic Profile of 22 Pancreatic Carcinoma Cell Lines. Analysis of K-ras, P53, P16 and DPC4/Smad4. Virchows Arch. 2001;439(6):798-802. PubMed PMID: 11787853.
    * Article titles in AMA citation format should be in sentence-case
    TY - JOUR T1 - Genetic profile of 22 pancreatic carcinoma cell lines. Analysis of K-ras, p53, p16 and DPC4/Smad4. AU - Moore,P S, AU - Sipos,B, AU - Orlandini,S, AU - Sorio,C, AU - Real,F X, AU - Lemoine,N R, AU - Gress,T, AU - Bassi,C, AU - Klöppel,G, AU - Kalthoff,H, AU - Ungefroren,H, AU - Löhr,M, AU - Scarpa,A, PY - 2002/1/15/pubmed PY - 2002/1/26/medline PY - 2002/1/15/entrez SP - 798 EP - 802 JF - Virchows Archiv : an international journal of pathology JO - Virchows Arch. VL - 439 IS - 6 N2 - The K-ras, p53, p16 and DPC4 genes are among those most frequently altered in pancreatic ductal carcinoma. We analyzed 22 cell lines for alterations in these genes by direct sequence analysis and methylation-specific polymerase chain reaction. These cell lines showed mutations in K-ras and p53 at frequencies of 91% and 95%, respectively. Alterations in p16INK4a were found in all cases and included nine homozygous deletions, seven mutations and promoter methylation in six cases. Eight cell lines (36%) had an alteration of DPC4, including one mutation and seven homozygous deletions. The most typical mutational profile involved K-ras, p53, and p16INK4a, concurrently aberrated in 20 cases (91%). Eight cell lines had alterations in all four genes. Inactivation of DPC4 was always accompanied by alteration of all of the other three genes. This comprehensive data regarding the cumulative genetic alterations in pancreatic carcinoma cell lines will be of great value for studies involving drug sensitivity or resistance that may be associated with inactivation of a particular gene or molecular pathway. SN - 0945-6317 UR - https://www.unboundmedicine.com/medline/citation/11787853/Genetic_profile_of_22_pancreatic_carcinoma_cell_lines__Analysis_of_K_ras_p53_p16_and_DPC4/Smad4_ L2 - https://web.expasy.org/cgi-bin/cellosaurus/search?input=PubMed=11787853 DB - PRIME DP - Unbound Medicine ER -