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Effect of in vivo gene transfer of nNOS in the PVN on renal nerve discharge in rats.
Am J Physiol Heart Circ Physiol. 2002 Feb; 282(2):H594-601.AJ

Abstract

The paraventricular nucleus (PVN) of the hypothalamus is known to be involved in the control of sympathetic outflow. Nitric oxide (NO) has been shown to have a sympathoinhibitory effect in the PVN. The goal of the present study was to examine the influence of overexpression of neuronal NO synthase (nNOS) within the PVN on renal sympathetic nerve discharge (RSND). Adenovirus vectors encoding either nNOS (Ad.nNOS) or beta-galactosidase (Ad.beta-Gal) were transfected into the PVN in vivo. Initially, the dose of adenovirus needed for infection was determined from in vitro infection of cultured fibroblasts. In Ad.nNOS-treated rats, the local expression of nNOS within the PVN was confirmed by histochemistry for NADPH-diaphorase-positive neurons. There was a robust increase in staining of NADPH-diaphorase-positive cells in the PVN on the side injected with Ad.nNOS. The staining peaked at 3 days after injection of the virus. In alpha-chloralose- and urethane-anesthetized rats, microinjection of N(G)-monomethyl-L-arginine (L-NMMA), a NO antagonist, into the PVN produced a dose-dependent increase in RSND, blood pressure, and heart rate. There was a potentiation of the increase in RSND, blood pressure, and heart rate due to L-NMMA in Ad.nNOS-injected rats compared with Ad.beta-Gal-injected rats. These results suggest that the endogenous NO-mediated effect in the PVN of Ad.nNOS-treated rats is more effective in suppressing RSND compared with Ad.beta-Gal-treated rats. These observations support the contention that an overexpression of nNOS within the PVN may be responsible for increased suppression of sympathetic outflow. This technique may be useful in pathological conditions know to have increased sympathetic outflow, such as hypertension or heart failure.

Authors+Show Affiliations

Department of Physiology and Biophysics, University of Nebraska Medical Center, Omaha, Nebraska 68198-4575, USA.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, U.S. Gov't, P.H.S.

Language

eng

PubMed ID

11788407

Citation

Li, Yi-Fan, et al. "Effect of in Vivo Gene Transfer of nNOS in the PVN On Renal Nerve Discharge in Rats." American Journal of Physiology. Heart and Circulatory Physiology, vol. 282, no. 2, 2002, pp. H594-601.
Li YF, Roy SK, Channon KM, et al. Effect of in vivo gene transfer of nNOS in the PVN on renal nerve discharge in rats. Am J Physiol Heart Circ Physiol. 2002;282(2):H594-601.
Li, Y. F., Roy, S. K., Channon, K. M., Zucker, I. H., & Patel, K. P. (2002). Effect of in vivo gene transfer of nNOS in the PVN on renal nerve discharge in rats. American Journal of Physiology. Heart and Circulatory Physiology, 282(2), H594-601.
Li YF, et al. Effect of in Vivo Gene Transfer of nNOS in the PVN On Renal Nerve Discharge in Rats. Am J Physiol Heart Circ Physiol. 2002;282(2):H594-601. PubMed PMID: 11788407.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Effect of in vivo gene transfer of nNOS in the PVN on renal nerve discharge in rats. AU - Li,Yi-Fan, AU - Roy,Shyamal K, AU - Channon,Keith M, AU - Zucker,Irving H, AU - Patel,Kaushik P, PY - 2002/1/15/pubmed PY - 2002/2/15/medline PY - 2002/1/15/entrez SP - H594 EP - 601 JF - American journal of physiology. Heart and circulatory physiology JO - Am J Physiol Heart Circ Physiol VL - 282 IS - 2 N2 - The paraventricular nucleus (PVN) of the hypothalamus is known to be involved in the control of sympathetic outflow. Nitric oxide (NO) has been shown to have a sympathoinhibitory effect in the PVN. The goal of the present study was to examine the influence of overexpression of neuronal NO synthase (nNOS) within the PVN on renal sympathetic nerve discharge (RSND). Adenovirus vectors encoding either nNOS (Ad.nNOS) or beta-galactosidase (Ad.beta-Gal) were transfected into the PVN in vivo. Initially, the dose of adenovirus needed for infection was determined from in vitro infection of cultured fibroblasts. In Ad.nNOS-treated rats, the local expression of nNOS within the PVN was confirmed by histochemistry for NADPH-diaphorase-positive neurons. There was a robust increase in staining of NADPH-diaphorase-positive cells in the PVN on the side injected with Ad.nNOS. The staining peaked at 3 days after injection of the virus. In alpha-chloralose- and urethane-anesthetized rats, microinjection of N(G)-monomethyl-L-arginine (L-NMMA), a NO antagonist, into the PVN produced a dose-dependent increase in RSND, blood pressure, and heart rate. There was a potentiation of the increase in RSND, blood pressure, and heart rate due to L-NMMA in Ad.nNOS-injected rats compared with Ad.beta-Gal-injected rats. These results suggest that the endogenous NO-mediated effect in the PVN of Ad.nNOS-treated rats is more effective in suppressing RSND compared with Ad.beta-Gal-treated rats. These observations support the contention that an overexpression of nNOS within the PVN may be responsible for increased suppression of sympathetic outflow. This technique may be useful in pathological conditions know to have increased sympathetic outflow, such as hypertension or heart failure. SN - 0363-6135 UR - https://www.unboundmedicine.com/medline/citation/11788407/Effect_of_in_vivo_gene_transfer_of_nNOS_in_the_PVN_on_renal_nerve_discharge_in_rats_ L2 - https://journals.physiology.org/doi/10.1152/ajpheart.00503.2001?url_ver=Z39.88-2003&rfr_id=ori:rid:crossref.org&rfr_dat=cr_pub=pubmed DB - PRIME DP - Unbound Medicine ER -