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Blood flow-dependent changes in renal interstitial guanosine 3',5'-cyclic monophosphate in rabbits.
Am J Physiol Renal Physiol. 2002 Feb; 282(2):F238-44.AJ

Abstract

We examined responses of renal interstitial guanosine 3',5'-cyclic monophosphate (cGMP) to changes in renal perfusion pressure (RPP) within and below the range of renal blood flow (RBF) autoregulation. A microdialysis method was used to monitor renal cortical and medullary interstitial cGMP levels in anesthetized rabbits. RPP was reduced in two steps: from ambient pressure (89 +/- 3 mmHg) to 70 +/- 2 mmHg (step 1) and then to 48 +/- 3 mmHg (step 2). RBF was maintained in step 1 but was significantly decreased in step 2 from 2.94 +/- 0.23 to 1.47 +/- 0.08 ml x min(-1) x g(-1). Basal interstitial concentrations of cGMP were significantly lower in the cortex than in the medulla (12.1 +/- 1.4 and 19.9 +/- 0.4 nmol/l, respectively). Cortical and medullary cGMP did not change in step 1 but were significantly decreased in step 2, with significantly less reduction in cGMP concentrations in the medulla than in the cortex (-25 +/- 3 and -44 +/- 3%, respectively). Over this pressure range, changes in cortical and medullary cGMP were highly correlated with changes in RBF (r = 0.94, P < 0.005 for cortex; r = 0.82, P < 0.01 for medulla). Renal interstitial nitrate/nitrite was not changed in step 1 but was significantly decreased in step 2 (-38 +/- 2% in cortex and -20 +/- 2% in medulla). Nitric oxide synthase inhibition with N(G)-nitro-L-arginine methyl ester (L-NAME, 30 mg/kg bolus, 50 mg x kg(-1) x h(-1) i.v. infusion) significantly decreased RBF (by -46 +/- 4%) and interstitial concentrations of cGMP (-27 +/- 4% in cortex and -22 +/- 4% in medulla, respectively). During L-NAME treatment, renal interstitial concentrations of cGMP in the cortex and medulla were similarly not altered in step 1. However, L-NAME significantly attenuated cGMP responses to a reduction in RPP in step 2. These results indicate that acute changes in RBF result in alterations in nitric oxide-dependent renal interstitial cGMP levels, with differential effects in the medulla compared with the cortex.

Authors+Show Affiliations

Department of Pharmacology, Kagawa Medical University, Kagawa 761-0793, Japan. yakuri@kms.ac.jpNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

11788437

Citation

Nishiyama, Akira, et al. "Blood Flow-dependent Changes in Renal Interstitial Guanosine 3',5'-cyclic Monophosphate in Rabbits." American Journal of Physiology. Renal Physiology, vol. 282, no. 2, 2002, pp. F238-44.
Nishiyama A, Kimura S, Fukui T, et al. Blood flow-dependent changes in renal interstitial guanosine 3',5'-cyclic monophosphate in rabbits. Am J Physiol Renal Physiol. 2002;282(2):F238-44.
Nishiyama, A., Kimura, S., Fukui, T., Rahman, M., Yoneyama, H., Kosaka, H., & Abe, Y. (2002). Blood flow-dependent changes in renal interstitial guanosine 3',5'-cyclic monophosphate in rabbits. American Journal of Physiology. Renal Physiology, 282(2), F238-44.
Nishiyama A, et al. Blood Flow-dependent Changes in Renal Interstitial Guanosine 3',5'-cyclic Monophosphate in Rabbits. Am J Physiol Renal Physiol. 2002;282(2):F238-44. PubMed PMID: 11788437.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Blood flow-dependent changes in renal interstitial guanosine 3',5'-cyclic monophosphate in rabbits. AU - Nishiyama,Akira, AU - Kimura,Shoji, AU - Fukui,Toshiki, AU - Rahman,Matlubur, AU - Yoneyama,Hirohito, AU - Kosaka,Hiroaki, AU - Abe,Youichi, PY - 2002/1/15/pubmed PY - 2002/2/22/medline PY - 2002/1/15/entrez SP - F238 EP - 44 JF - American journal of physiology. Renal physiology JO - Am J Physiol Renal Physiol VL - 282 IS - 2 N2 - We examined responses of renal interstitial guanosine 3',5'-cyclic monophosphate (cGMP) to changes in renal perfusion pressure (RPP) within and below the range of renal blood flow (RBF) autoregulation. A microdialysis method was used to monitor renal cortical and medullary interstitial cGMP levels in anesthetized rabbits. RPP was reduced in two steps: from ambient pressure (89 +/- 3 mmHg) to 70 +/- 2 mmHg (step 1) and then to 48 +/- 3 mmHg (step 2). RBF was maintained in step 1 but was significantly decreased in step 2 from 2.94 +/- 0.23 to 1.47 +/- 0.08 ml x min(-1) x g(-1). Basal interstitial concentrations of cGMP were significantly lower in the cortex than in the medulla (12.1 +/- 1.4 and 19.9 +/- 0.4 nmol/l, respectively). Cortical and medullary cGMP did not change in step 1 but were significantly decreased in step 2, with significantly less reduction in cGMP concentrations in the medulla than in the cortex (-25 +/- 3 and -44 +/- 3%, respectively). Over this pressure range, changes in cortical and medullary cGMP were highly correlated with changes in RBF (r = 0.94, P < 0.005 for cortex; r = 0.82, P < 0.01 for medulla). Renal interstitial nitrate/nitrite was not changed in step 1 but was significantly decreased in step 2 (-38 +/- 2% in cortex and -20 +/- 2% in medulla). Nitric oxide synthase inhibition with N(G)-nitro-L-arginine methyl ester (L-NAME, 30 mg/kg bolus, 50 mg x kg(-1) x h(-1) i.v. infusion) significantly decreased RBF (by -46 +/- 4%) and interstitial concentrations of cGMP (-27 +/- 4% in cortex and -22 +/- 4% in medulla, respectively). During L-NAME treatment, renal interstitial concentrations of cGMP in the cortex and medulla were similarly not altered in step 1. However, L-NAME significantly attenuated cGMP responses to a reduction in RPP in step 2. These results indicate that acute changes in RBF result in alterations in nitric oxide-dependent renal interstitial cGMP levels, with differential effects in the medulla compared with the cortex. SN - 1931-857X UR - https://www.unboundmedicine.com/medline/citation/11788437/Blood_flow_dependent_changes_in_renal_interstitial_guanosine_3'5'_cyclic_monophosphate_in_rabbits_ L2 - https://journals.physiology.org/doi/10.1152/ajprenal.00087.2001?url_ver=Z39.88-2003&amp;rfr_id=ori:rid:crossref.org&amp;rfr_dat=cr_pub=pubmed DB - PRIME DP - Unbound Medicine ER -