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Renal arginine and protein synthesis are increased during early endotoxemia in mice.
Am J Physiol Renal Physiol. 2002 Feb; 282(2):F316-23.AJ

Abstract

The kidney has an important function in arginine metabolism, because the kidney is the main endogenous source for de novo arginine production from circulating citrulline. In conditions such as sepsis, nitric oxide (NO) production is increased and is dependent on extracellular arginine availability. To elucidate the adaptive role of renal de novo arginine synthesis in a condition of increased NO production, we studied renal arginine metabolism in a mouse model of endotoxemia. Because arginine flux is largely dependent on protein flux, we also measured protein metabolism in mice. Female mice were injected intraperitoneally with lipopolysaccharide; control mice received 0.9% NaCl. Six hours later, renal blood flow was measured with the use of para-aminohippuric acid. Arginine and protein metabolism were studied using organ-balance, stable-isotope techniques. Systemic NO production was increased in the endotoxin-treated mice. In addition, renal protein synthesis and de novo arginine production from citrulline were increased. However, no effect on renal NO production was observed. In conclusion, increased renal de novo arginine production may serve to sustain systemic NO production. To our knowledge, it was shown for the first time that renal protein synthesis is enhanced in the early response to endotoxemia.

Authors+Show Affiliations

Department of Surgery, Maastricht University, 6200 MD Maastricht, The Netherlands.No affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

11788446

Citation

Hallemeesch, Marcella M., et al. "Renal Arginine and Protein Synthesis Are Increased During Early Endotoxemia in Mice." American Journal of Physiology. Renal Physiology, vol. 282, no. 2, 2002, pp. F316-23.
Hallemeesch MM, Soeters PB, Deutz NE. Renal arginine and protein synthesis are increased during early endotoxemia in mice. Am J Physiol Renal Physiol. 2002;282(2):F316-23.
Hallemeesch, M. M., Soeters, P. B., & Deutz, N. E. (2002). Renal arginine and protein synthesis are increased during early endotoxemia in mice. American Journal of Physiology. Renal Physiology, 282(2), F316-23.
Hallemeesch MM, Soeters PB, Deutz NE. Renal Arginine and Protein Synthesis Are Increased During Early Endotoxemia in Mice. Am J Physiol Renal Physiol. 2002;282(2):F316-23. PubMed PMID: 11788446.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Renal arginine and protein synthesis are increased during early endotoxemia in mice. AU - Hallemeesch,Marcella M, AU - Soeters,Peter B, AU - Deutz,Nicolaas E P, PY - 2002/1/15/pubmed PY - 2002/2/22/medline PY - 2002/1/15/entrez SP - F316 EP - 23 JF - American journal of physiology. Renal physiology JO - Am. J. Physiol. Renal Physiol. VL - 282 IS - 2 N2 - The kidney has an important function in arginine metabolism, because the kidney is the main endogenous source for de novo arginine production from circulating citrulline. In conditions such as sepsis, nitric oxide (NO) production is increased and is dependent on extracellular arginine availability. To elucidate the adaptive role of renal de novo arginine synthesis in a condition of increased NO production, we studied renal arginine metabolism in a mouse model of endotoxemia. Because arginine flux is largely dependent on protein flux, we also measured protein metabolism in mice. Female mice were injected intraperitoneally with lipopolysaccharide; control mice received 0.9% NaCl. Six hours later, renal blood flow was measured with the use of para-aminohippuric acid. Arginine and protein metabolism were studied using organ-balance, stable-isotope techniques. Systemic NO production was increased in the endotoxin-treated mice. In addition, renal protein synthesis and de novo arginine production from citrulline were increased. However, no effect on renal NO production was observed. In conclusion, increased renal de novo arginine production may serve to sustain systemic NO production. To our knowledge, it was shown for the first time that renal protein synthesis is enhanced in the early response to endotoxemia. SN - 1931-857X UR - https://www.unboundmedicine.com/medline/citation/11788446/Renal_arginine_and_protein_synthesis_are_increased_during_early_endotoxemia_in_mice_ L2 - http://www.physiology.org/doi/full/10.1152/ajprenal.0039.2001?url_ver=Z39.88-2003&rfr_id=ori:rid:crossref.org&rfr_dat=cr_pub=pubmed DB - PRIME DP - Unbound Medicine ER -