Tags

Type your tag names separated by a space and hit enter

Dual regulation of mouse Delta(5)- and Delta(6)-desaturase gene expression by SREBP-1 and PPARalpha.
J Lipid Res. 2002 Jan; 43(1):107-14.JL

Abstract

In the process of seeking sterol regulatory element-binding protein 1a (SREBP-1a) target genes, we identified and cloned a cDNA clone encoding mouse Delta(5)-desaturase (D5D). The hepatic expression of D5D as well as Delta(6)-desaturase (D6D) was highly activated in transgenic mice overexpressing nuclear SREBP-1a, -1c, and -2. Disruption of the SREBP-1 gene significantly reduced the expression of both desaturases in the livers of SREBP-1-deficient mice refed after fasting. The hepatic expression of both desaturases was downregulated by dietary PUFA, which were reported to suppress SREBP-1c gene expression. Sustained expression of hepatic nuclear SREBP-1c protein in the transgenic mice abolished the PUFA suppression of both desaturases. Although these data suggested that SREBP-1c regulates D5D and D6D expression, there was no difference in either the D5D or D6D mRNA level between fasted and refed normal mouse livers, indicating a mechanism for fasting induction of both desaturases. Administration of fibrate, a pharmacological ligand for peroxisome proliferator activating receptor alpha (PPARalpha), caused a significant increase in expression of both desaturases. The data suggested that D5D and D6D expression is dually regulated by SREBP-1c and PPARalpha, two reciprocal transcription factors for fatty acid metabolism, and could be involved in lipogenic gene regulation by producing PUFA.

Authors+Show Affiliations

Department of Metabolic Diseases, University of Tokyo, 7-3-1 Hongo, Bunkyo-ku, Tokyo, 113-8655, Japan.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

11792729

Citation

Matsuzaka, Takashi, et al. "Dual Regulation of Mouse Delta(5)- and Delta(6)-desaturase Gene Expression By SREBP-1 and PPARalpha." Journal of Lipid Research, vol. 43, no. 1, 2002, pp. 107-14.
Matsuzaka T, Shimano H, Yahagi N, et al. Dual regulation of mouse Delta(5)- and Delta(6)-desaturase gene expression by SREBP-1 and PPARalpha. J Lipid Res. 2002;43(1):107-14.
Matsuzaka, T., Shimano, H., Yahagi, N., Amemiya-Kudo, M., Yoshikawa, T., Hasty, A. H., Tamura, Y., Osuga, J., Okazaki, H., Iizuka, Y., Takahashi, A., Sone, H., Gotoda, T., Ishibashi, S., & Yamada, N. (2002). Dual regulation of mouse Delta(5)- and Delta(6)-desaturase gene expression by SREBP-1 and PPARalpha. Journal of Lipid Research, 43(1), 107-14.
Matsuzaka T, et al. Dual Regulation of Mouse Delta(5)- and Delta(6)-desaturase Gene Expression By SREBP-1 and PPARalpha. J Lipid Res. 2002;43(1):107-14. PubMed PMID: 11792729.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Dual regulation of mouse Delta(5)- and Delta(6)-desaturase gene expression by SREBP-1 and PPARalpha. AU - Matsuzaka,Takashi, AU - Shimano,Hitoshi, AU - Yahagi,Naoya, AU - Amemiya-Kudo,Michiyo, AU - Yoshikawa,Tomohiro, AU - Hasty,Alyssa H, AU - Tamura,Yoshiaki, AU - Osuga,Jun-ichi, AU - Okazaki,Hiroaki, AU - Iizuka,Yoko, AU - Takahashi,Akimitsu, AU - Sone,Hirohito, AU - Gotoda,Takanari, AU - Ishibashi,Shun, AU - Yamada,Nobuhiro, PY - 2002/1/17/pubmed PY - 2002/5/1/medline PY - 2002/1/17/entrez SP - 107 EP - 14 JF - Journal of lipid research JO - J Lipid Res VL - 43 IS - 1 N2 - In the process of seeking sterol regulatory element-binding protein 1a (SREBP-1a) target genes, we identified and cloned a cDNA clone encoding mouse Delta(5)-desaturase (D5D). The hepatic expression of D5D as well as Delta(6)-desaturase (D6D) was highly activated in transgenic mice overexpressing nuclear SREBP-1a, -1c, and -2. Disruption of the SREBP-1 gene significantly reduced the expression of both desaturases in the livers of SREBP-1-deficient mice refed after fasting. The hepatic expression of both desaturases was downregulated by dietary PUFA, which were reported to suppress SREBP-1c gene expression. Sustained expression of hepatic nuclear SREBP-1c protein in the transgenic mice abolished the PUFA suppression of both desaturases. Although these data suggested that SREBP-1c regulates D5D and D6D expression, there was no difference in either the D5D or D6D mRNA level between fasted and refed normal mouse livers, indicating a mechanism for fasting induction of both desaturases. Administration of fibrate, a pharmacological ligand for peroxisome proliferator activating receptor alpha (PPARalpha), caused a significant increase in expression of both desaturases. The data suggested that D5D and D6D expression is dually regulated by SREBP-1c and PPARalpha, two reciprocal transcription factors for fatty acid metabolism, and could be involved in lipogenic gene regulation by producing PUFA. SN - 0022-2275 UR - https://www.unboundmedicine.com/medline/citation/11792729/Dual_regulation_of_mouse_Delta_5___and_Delta_6__desaturase_gene_expression_by_SREBP_1_and_PPARalpha_ L2 - http://www.jlr.org/cgi/pmidlookup?view=long&pmid=11792729 DB - PRIME DP - Unbound Medicine ER -