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Genes active in developing wheat endosperm.
Funct Integr Genomics. 2000 May; 1(1):44-55.FI

Abstract

This paper describes the construction and characterisation of a cDNA library from wheat endosperm tissue during the early stages of grain filling. Developing wheat endosperm tissue was characterised with respect to standard measures including dry weight, cytological appearance and timing of expression of major sources of mRNA such as the seed storage protein genes. In addition, the full complement of proteins present at mid-endosperm development was examined using 2D-electrophoretic techniques. Based on this characterisation, endosperm from the developing grain 8-12 days post-anthesis was chosen for isolating mRNA and preparing cDNA. At this stage in development the mRNA population is not yet dominated by the accumulation of mRNA from seed storage protein genes. A cDNA library, not normalised, containing a high percentage of full length cDNA clones was constructed and 4,319 clones sequenced ("single-pass"). Partitioning of the cDNA sequences into gene families and singletons provided the basis for quantifying the accumulation of sequence classes relative to the total number of sequences determined. The accumulation of gene families/singletons was not linear. However, mathematical modeling of the data suggested that the maximum number of different genes expressed is within the range of 4,500-8,000 (detailed in the Appendix). If an average is taken of these extremes, approximately 27% of the gene products were visible as proteins in the 2D-electrophoretic analysis. Analysis of a functional class of genes relevant to wheat grain end-use, namely the glutenin/gliadin seed storage protein class of genes, revealed a new category of gene characterised by a distinctive N-terminal domain and a reduced central repetitive domain.

Authors+Show Affiliations

Clarke BC
CSIRO Plant Industry, PO Box 1600, Canberra, ACT 2601, Australia. b.clarke@pi.csiro.au
Hobbs M
No affiliation info available
Skylas D
No affiliation info available
Appels R
No affiliation info available

MeSH

Amino Acid SequenceBase SequenceDNA, PlantExpressed Sequence TagsGene LibraryGenes, PlantMicrosatellite RepeatsModels, TheoreticalMolecular Sequence DataNonlinear DynamicsNucleic Acid HybridizationOligonucleotide Array Sequence AnalysisPlant ProteinsProtein Structure, TertiarySeedsSequence AlignmentSequence Analysis, DNATime FactorsTranscription, GeneticTriticum

Pub Type(s)

Comparative Study
Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

11793221

Citation

Clarke, B C., et al. "Genes Active in Developing Wheat Endosperm." Functional & Integrative Genomics, vol. 1, no. 1, 2000, pp. 44-55.
Clarke BC, Hobbs M, Skylas D, et al. Genes active in developing wheat endosperm. Funct Integr Genomics. 2000;1(1):44-55.
Clarke, B. C., Hobbs, M., Skylas, D., & Appels, R. (2000). Genes active in developing wheat endosperm. Functional & Integrative Genomics, 1(1), 44-55.
Clarke BC, et al. Genes Active in Developing Wheat Endosperm. Funct Integr Genomics. 2000;1(1):44-55. PubMed PMID: 11793221.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Genes active in developing wheat endosperm. AU - Clarke,B C, AU - Hobbs,M, AU - Skylas,D, AU - Appels,R, PY - 1999/10/20/received PY - 2000/02/04/accepted PY - 2002/1/17/pubmed PY - 2002/3/23/medline PY - 2002/1/17/entrez SP - 44 EP - 55 JF - Functional & integrative genomics JO - Funct Integr Genomics VL - 1 IS - 1 N2 - This paper describes the construction and characterisation of a cDNA library from wheat endosperm tissue during the early stages of grain filling. Developing wheat endosperm tissue was characterised with respect to standard measures including dry weight, cytological appearance and timing of expression of major sources of mRNA such as the seed storage protein genes. In addition, the full complement of proteins present at mid-endosperm development was examined using 2D-electrophoretic techniques. Based on this characterisation, endosperm from the developing grain 8-12 days post-anthesis was chosen for isolating mRNA and preparing cDNA. At this stage in development the mRNA population is not yet dominated by the accumulation of mRNA from seed storage protein genes. A cDNA library, not normalised, containing a high percentage of full length cDNA clones was constructed and 4,319 clones sequenced ("single-pass"). Partitioning of the cDNA sequences into gene families and singletons provided the basis for quantifying the accumulation of sequence classes relative to the total number of sequences determined. The accumulation of gene families/singletons was not linear. However, mathematical modeling of the data suggested that the maximum number of different genes expressed is within the range of 4,500-8,000 (detailed in the Appendix). If an average is taken of these extremes, approximately 27% of the gene products were visible as proteins in the 2D-electrophoretic analysis. Analysis of a functional class of genes relevant to wheat grain end-use, namely the glutenin/gliadin seed storage protein class of genes, revealed a new category of gene characterised by a distinctive N-terminal domain and a reduced central repetitive domain. SN - 1438-793X UR - https://www.unboundmedicine.com/medline/citation/11793221/Genes_active_in_developing_wheat_endosperm_ L2 - https://dx.doi.org/10.1007/s101420000008 DB - PRIME DP - Unbound Medicine ER -