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[Synergistic effect of monocyte chemotactic protein-1 and aristolochic acid I on transdifferentiation of human tubular epithelial cells in vitro].
Zhonghua Nei Ke Za Zhi. 2000 Dec; 39(12):831-4.ZN

Abstract

OBJECTIVE

To test the possible role of monocyte chemotactic protein-1 (MCP-1) and its synergistic effect with aristolochic acid I (AAI) on tubular epithelial-myofibroblast transdifferentiation (TEMT) of human renal tubular epithelial cells (HKC) in vitro.

METHODS

The cultured HKC cells were divided into four groups: (1) negative control (serum-free); (2) MCP-1 group; (3) AAI group; (4) AAI + MCP-1 group. The expression of alpha-smooth muscle actin (alpha-SMA), vimentin and cytocreatin was assessed by indirect enzyme immunohistochemistry and the percentages of alpha-SMA((+)) HKC cells were assessed by flow cytometry.

RESULTS

The expression of cytocreatin of HKC cells decreased, while the expression of alpha-SMA, vimentin increased when treated with MCP-1 or with AAI and MCP-I concomitantly. Alpha-SMA((+)) HKC cells cultured in serum-free medium was 3.1% by flow cytometry. The percentages of alpha-SMA((+)) HKC cells were 8.6%, 9.6%, 13.4% (P < 0.05 vs control) when treated with 20, 40, 80 microg/L of AAI. The percentages of alpha-SMA((+)) HKC cells were 0.5% and 1.4% (P > 0.05 vs control) when treated with 5, 10 microg/L. The percentages of alpha-SMA((+)) HKC cells were 20%, 26.2%, 20.3%, 23.2% (P < 0.05 vs control) when treated with 0.001, 0.01, 0.05, 0.1 microg/L of MCP-1. When the HKC cells were treated with MCP-1 (0.1 microg/L) and AAI (5, 10, 20, 40 microg/L), the percentage of alpha-SMA((+)) cells increased markedly to 23.2%, 98.7%, 81.5%, 65.1% (P < 0. 01 vs group 1, 2, 3, respectively).

CONCLUSIONS

These findings suggest that: (1) MCP-1 may induce the transdifferentiation of HKC cells into myofibroblasts in vitro; (2) AAI at some doses may partially induce HKC cells transdifferentiation. (3) MCP-1 and AAI may have a synergistic effect on transdifferentiation of HKC cells in vitro.

Authors+Show Affiliations

Division of Nephrology, Peking Union Medical College Hospital, Peking Union Medical College, Chinese Academy of Medical Sciences, Beijing 100730, China.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

chi

PubMed ID

11798547

Citation

Zheng, F, et al. "[Synergistic Effect of Monocyte Chemotactic Protein-1 and Aristolochic Acid I On Transdifferentiation of Human Tubular Epithelial Cells in Vitro]." Zhonghua Nei Ke Za Zhi, vol. 39, no. 12, 2000, pp. 831-4.
Zheng F, Wen X, Li X, et al. [Synergistic effect of monocyte chemotactic protein-1 and aristolochic acid I on transdifferentiation of human tubular epithelial cells in vitro]. Zhonghua Nei Ke Za Zhi. 2000;39(12):831-4.
Zheng, F., Wen, X., Li, X., Gao, R., & Zhang, X. (2000). [Synergistic effect of monocyte chemotactic protein-1 and aristolochic acid I on transdifferentiation of human tubular epithelial cells in vitro]. Zhonghua Nei Ke Za Zhi, 39(12), 831-4.
Zheng F, et al. [Synergistic Effect of Monocyte Chemotactic Protein-1 and Aristolochic Acid I On Transdifferentiation of Human Tubular Epithelial Cells in Vitro]. Zhonghua Nei Ke Za Zhi. 2000;39(12):831-4. PubMed PMID: 11798547.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - [Synergistic effect of monocyte chemotactic protein-1 and aristolochic acid I on transdifferentiation of human tubular epithelial cells in vitro]. AU - Zheng,F, AU - Wen,X, AU - Li,X, AU - Gao,R, AU - Zhang,X, PY - 2002/1/19/pubmed PY - 2003/7/11/medline PY - 2002/1/19/entrez SP - 831 EP - 4 JF - Zhonghua nei ke za zhi JO - Zhonghua Nei Ke Za Zhi VL - 39 IS - 12 N2 - OBJECTIVE: To test the possible role of monocyte chemotactic protein-1 (MCP-1) and its synergistic effect with aristolochic acid I (AAI) on tubular epithelial-myofibroblast transdifferentiation (TEMT) of human renal tubular epithelial cells (HKC) in vitro. METHODS: The cultured HKC cells were divided into four groups: (1) negative control (serum-free); (2) MCP-1 group; (3) AAI group; (4) AAI + MCP-1 group. The expression of alpha-smooth muscle actin (alpha-SMA), vimentin and cytocreatin was assessed by indirect enzyme immunohistochemistry and the percentages of alpha-SMA((+)) HKC cells were assessed by flow cytometry. RESULTS: The expression of cytocreatin of HKC cells decreased, while the expression of alpha-SMA, vimentin increased when treated with MCP-1 or with AAI and MCP-I concomitantly. Alpha-SMA((+)) HKC cells cultured in serum-free medium was 3.1% by flow cytometry. The percentages of alpha-SMA((+)) HKC cells were 8.6%, 9.6%, 13.4% (P < 0.05 vs control) when treated with 20, 40, 80 microg/L of AAI. The percentages of alpha-SMA((+)) HKC cells were 0.5% and 1.4% (P > 0.05 vs control) when treated with 5, 10 microg/L. The percentages of alpha-SMA((+)) HKC cells were 20%, 26.2%, 20.3%, 23.2% (P < 0.05 vs control) when treated with 0.001, 0.01, 0.05, 0.1 microg/L of MCP-1. When the HKC cells were treated with MCP-1 (0.1 microg/L) and AAI (5, 10, 20, 40 microg/L), the percentage of alpha-SMA((+)) cells increased markedly to 23.2%, 98.7%, 81.5%, 65.1% (P < 0. 01 vs group 1, 2, 3, respectively). CONCLUSIONS: These findings suggest that: (1) MCP-1 may induce the transdifferentiation of HKC cells into myofibroblasts in vitro; (2) AAI at some doses may partially induce HKC cells transdifferentiation. (3) MCP-1 and AAI may have a synergistic effect on transdifferentiation of HKC cells in vitro. SN - 0578-1426 UR - https://www.unboundmedicine.com/medline/citation/11798547/[Synergistic_effect_of_monocyte_chemotactic_protein_1_and_aristolochic_acid_I_on_transdifferentiation_of_human_tubular_epithelial_cells_in_vitro]_ L2 - https://antibodies.cancer.gov/detail/CPTC-OTUB1-1 DB - PRIME DP - Unbound Medicine ER -