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[Placental leptin correlates with intrauterine fetal growth and development].
Zhonghua Yi Xue Za Zhi 2001; 81(8):489-92ZY

Abstract

OBJECTIVE

To study the role of placental leptin in intrauterine cord leptin production and its relationship with neonatal anthropometry.

METHODS

Forty women and their babies were enrolled and approved by Xinhua Hospital (Shanghai, China) and Jiangbei Hospital (Nanjing, China) in this study. Placental tissue was assayed for leptin mRNA by reverse transcription/polymerase chain reaction (RT/PCR), and assayed for ob gene protein, leptin, by Western-Blot and immunohistochemistry. Blood was taken from the umbilical cord of the babies at delivery. Serum leptin was measured by radio-immunoassay. Neonatal anthropometric measurements were recorded within 48 hours after delivery. Linear regression analysis was used to explore the relationship between placental leptin, cord leptin and neonatal anthropometric measures.

RESULTS

Ob gene was expressed in placental tissue at comparable or greater levels than that in adipose tissue. Comparison of the relative levels of leptin to beta-actin mRNA by multiplex RT/PCR revealed that the placenta of the small for gestational age (SGA) neonates expressed leptin mRNA at significantly lower levels 0.61 +/- 0.15 than that of the appropriate for gestational age (AGA) neonates 0.83 +/- 0.20 (P = 0.0034), while the placenta of the large for gestational age (LGA) neonates expressed leptin mRNA at significantly higher levels 1.00 +/- 0.23 than that of the AGA neonates (P = 0.043). Immunohistochemical techniques showed the immunostaining pattern in the cytoplasm of trophoblastic cells. Western-blot showed that the placenta of the SGA neonates expressed leptin at significantly lower levels 0.26 +/- 0.05 ng/mg than that of the AGA neonates 0.34 +/- 0.09 ng/mg (P = 0.007 6), while the placenta of the LGA neonates expressed leptin at significantly higher levels 0.43 +/- 0.10 ng/mg than that of the AGA neonates (P = 0.021). Linear regression analysis showed placental ob gene transcription and leptin translation correlated significantly with cord leptin (r = 0.39 and 0.43), and neonatal Ponderal Index (r = 0.66 and 0.69).

CONCLUSIONS

Placenta provides a source of leptin for the growing fetus, and this placental leptin might be a growth factor in intrauterine fetal development.

Authors+Show Affiliations

Shanghai Institute for Pediatric Research, Xinhua Hospital Shanghai Second Medical University, Shanghai 200092, China.No affiliation info availableNo affiliation info available

Pub Type(s)

English Abstract
Journal Article

Language

chi

PubMed ID

11798925

Citation

Ben, X, et al. "[Placental Leptin Correlates With Intrauterine Fetal Growth and Development]." Zhonghua Yi Xue Za Zhi, vol. 81, no. 8, 2001, pp. 489-92.
Ben X, Qin Y, Wu S. [Placental leptin correlates with intrauterine fetal growth and development]. Zhonghua Yi Xue Za Zhi. 2001;81(8):489-92.
Ben, X., Qin, Y., & Wu, S. (2001). [Placental leptin correlates with intrauterine fetal growth and development]. Zhonghua Yi Xue Za Zhi, 81(8), pp. 489-92.
Ben X, Qin Y, Wu S. [Placental Leptin Correlates With Intrauterine Fetal Growth and Development]. Zhonghua Yi Xue Za Zhi. 2001 Apr 25;81(8):489-92. PubMed PMID: 11798925.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - [Placental leptin correlates with intrauterine fetal growth and development]. AU - Ben,X, AU - Qin,Y, AU - Wu,S, PY - 2002/1/19/pubmed PY - 2002/5/1/medline PY - 2002/1/19/entrez SP - 489 EP - 92 JF - Zhonghua yi xue za zhi JO - Zhonghua Yi Xue Za Zhi VL - 81 IS - 8 N2 - OBJECTIVE: To study the role of placental leptin in intrauterine cord leptin production and its relationship with neonatal anthropometry. METHODS: Forty women and their babies were enrolled and approved by Xinhua Hospital (Shanghai, China) and Jiangbei Hospital (Nanjing, China) in this study. Placental tissue was assayed for leptin mRNA by reverse transcription/polymerase chain reaction (RT/PCR), and assayed for ob gene protein, leptin, by Western-Blot and immunohistochemistry. Blood was taken from the umbilical cord of the babies at delivery. Serum leptin was measured by radio-immunoassay. Neonatal anthropometric measurements were recorded within 48 hours after delivery. Linear regression analysis was used to explore the relationship between placental leptin, cord leptin and neonatal anthropometric measures. RESULTS: Ob gene was expressed in placental tissue at comparable or greater levels than that in adipose tissue. Comparison of the relative levels of leptin to beta-actin mRNA by multiplex RT/PCR revealed that the placenta of the small for gestational age (SGA) neonates expressed leptin mRNA at significantly lower levels 0.61 +/- 0.15 than that of the appropriate for gestational age (AGA) neonates 0.83 +/- 0.20 (P = 0.0034), while the placenta of the large for gestational age (LGA) neonates expressed leptin mRNA at significantly higher levels 1.00 +/- 0.23 than that of the AGA neonates (P = 0.043). Immunohistochemical techniques showed the immunostaining pattern in the cytoplasm of trophoblastic cells. Western-blot showed that the placenta of the SGA neonates expressed leptin at significantly lower levels 0.26 +/- 0.05 ng/mg than that of the AGA neonates 0.34 +/- 0.09 ng/mg (P = 0.007 6), while the placenta of the LGA neonates expressed leptin at significantly higher levels 0.43 +/- 0.10 ng/mg than that of the AGA neonates (P = 0.021). Linear regression analysis showed placental ob gene transcription and leptin translation correlated significantly with cord leptin (r = 0.39 and 0.43), and neonatal Ponderal Index (r = 0.66 and 0.69). CONCLUSIONS: Placenta provides a source of leptin for the growing fetus, and this placental leptin might be a growth factor in intrauterine fetal development. SN - 0376-2491 UR - https://www.unboundmedicine.com/medline/citation/11798925/[Placental_leptin_correlates_with_intrauterine_fetal_growth_and_development]_ L2 - http://journal.yiigle.com/LinkIn.do?linkin_type=pubmed&issn=0376-2491&year=2001&vol=81&issue=8&fpage=489 DB - PRIME DP - Unbound Medicine ER -