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Spider silk fibers spun from soluble recombinant silk produced in mammalian cells.
Science 2002; 295(5554):472-6Sci

Abstract

Spider silks are protein-based "biopolymer" filaments or threads secreted by specialized epithelial cells as concentrated soluble precursors of highly repetitive primary sequences. Spider dragline silk is a flexible, lightweight fiber of extraordinary strength and toughness comparable to that of synthetic high-performance fibers. We sought to "biomimic" the process of spider silk production by expressing in mammalian cells the dragline silk genes (ADF-3/MaSpII and MaSpI) of two spider species. We produced soluble recombinant (rc)-dragline silk proteins with molecular masses of 60 to 140 kilodaltons. We demonstrated the wet spinning of silk monofilaments spun from a concentrated aqueous solution of soluble rc-spider silk protein (ADF-3; 60 kilodaltons) under modest shear and coagulation conditions. The spun fibers were water insoluble with a fine diameter (10 to 40 micrometers) and exhibited toughness and modulus values comparable to those of native dragline silks but with lower tenacity. Dope solutions with rc-silk protein concentrations >20% and postspinning draw were necessary to achieve improved mechanical properties of the spun fibers. Fiber properties correlated with finer fiber diameter and increased birefringence.

Authors+Show Affiliations

Nexia Biotechnologies, Vaudreuil-Dorion, Quebec J7V 8P5, Canada. alazaris@nexiabiotech.comNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

11799236

Citation

Lazaris, Anthoula, et al. "Spider Silk Fibers Spun From Soluble Recombinant Silk Produced in Mammalian Cells." Science (New York, N.Y.), vol. 295, no. 5554, 2002, pp. 472-6.
Lazaris A, Arcidiacono S, Huang Y, et al. Spider silk fibers spun from soluble recombinant silk produced in mammalian cells. Science. 2002;295(5554):472-6.
Lazaris, A., Arcidiacono, S., Huang, Y., Zhou, J. F., Duguay, F., Chretien, N., ... Karatzas, C. N. (2002). Spider silk fibers spun from soluble recombinant silk produced in mammalian cells. Science (New York, N.Y.), 295(5554), pp. 472-6.
Lazaris A, et al. Spider Silk Fibers Spun From Soluble Recombinant Silk Produced in Mammalian Cells. Science. 2002 Jan 18;295(5554):472-6. PubMed PMID: 11799236.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Spider silk fibers spun from soluble recombinant silk produced in mammalian cells. AU - Lazaris,Anthoula, AU - Arcidiacono,Steven, AU - Huang,Yue, AU - Zhou,Jiang-Feng, AU - Duguay,Francois, AU - Chretien,Nathalie, AU - Welsh,Elizabeth A, AU - Soares,Jason W, AU - Karatzas,Costas N, PY - 2002/1/19/pubmed PY - 2002/2/9/medline PY - 2002/1/19/entrez SP - 472 EP - 6 JF - Science (New York, N.Y.) JO - Science VL - 295 IS - 5554 N2 - Spider silks are protein-based "biopolymer" filaments or threads secreted by specialized epithelial cells as concentrated soluble precursors of highly repetitive primary sequences. Spider dragline silk is a flexible, lightweight fiber of extraordinary strength and toughness comparable to that of synthetic high-performance fibers. We sought to "biomimic" the process of spider silk production by expressing in mammalian cells the dragline silk genes (ADF-3/MaSpII and MaSpI) of two spider species. We produced soluble recombinant (rc)-dragline silk proteins with molecular masses of 60 to 140 kilodaltons. We demonstrated the wet spinning of silk monofilaments spun from a concentrated aqueous solution of soluble rc-spider silk protein (ADF-3; 60 kilodaltons) under modest shear and coagulation conditions. The spun fibers were water insoluble with a fine diameter (10 to 40 micrometers) and exhibited toughness and modulus values comparable to those of native dragline silks but with lower tenacity. Dope solutions with rc-silk protein concentrations >20% and postspinning draw were necessary to achieve improved mechanical properties of the spun fibers. Fiber properties correlated with finer fiber diameter and increased birefringence. SN - 1095-9203 UR - https://www.unboundmedicine.com/medline/citation/11799236/Spider_silk_fibers_spun_from_soluble_recombinant_silk_produced_in_mammalian_cells_ L2 - http://www.sciencemag.org/cgi/pmidlookup?view=long&pmid=11799236 DB - PRIME DP - Unbound Medicine ER -