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Essential role(s) of the intrarenal renin-angiotensin system in transforming growth factor-beta1 gene expression and induction of hypertrophy of rat kidney proximal tubular cells in high glucose.
J Am Soc Nephrol. 2002 Feb; 13(2):302-12.JA

Abstract

These studies investigated the question of whether the intrarenal renin-angiotensin system (RAS) is essential for transforming growth factor-beta1 (TGF-beta1) gene expression and induction of hypertrophy of renal proximal tubular cells in high glucose in vitro. Antisense and sense angiotensinogen (ANG) cDNAs were stably transfected into rat immortalized renal proximal tubular cells (IRPTC). ANG secretion from rat IRPTC was quantified by a specific RIA for rat ANG. Cellular ANG, TGF-beta1, and collagen alpha1 (type IV) mRNA levels were determined by Northern blot analysis or by reverse transcriptase-PCR assay. Hypertrophy of IRPTC was analyzed by Western blotting of cellular p27(Kip1) protein, flow cytometry, and cellular protein assay. The results showed that stable transfer of antisense ANG cDNA into IRPTC suppressed the basal TGF-beta1 and collagen alpha1 (type IV) mRNA expression and blocked the stimulatory effect of high glucose (i.e., 25 mM) on TGF-beta1 and collagen alpha1 (type IV) mRNA expression and induction of IRPTC hypertrophy. In contrast, stable transfer of sense ANG cDNA into IRPTC had no significant effect on these parameters. These data demonstrate that local intrarenal RAS activation is essential for TGF-beta1 gene expression and induction of hypertrophy of renal proximal tubular cells in high glucose.

Authors+Show Affiliations

Université de Montréal, Centre hospitalier de l'Université de Montréal-Hôtel-Dieu and Hôpital Maisonneuve-Rosemont, Montreal, Quebec, Canada.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't
Research Support, U.S. Gov't, P.H.S.

Language

eng

PubMed ID

11805157

Citation

Zhang, Shao-Ling, et al. "Essential Role(s) of the Intrarenal Renin-angiotensin System in Transforming Growth Factor-beta1 Gene Expression and Induction of Hypertrophy of Rat Kidney Proximal Tubular Cells in High Glucose." Journal of the American Society of Nephrology : JASN, vol. 13, no. 2, 2002, pp. 302-12.
Zhang SL, To C, Chen X, et al. Essential role(s) of the intrarenal renin-angiotensin system in transforming growth factor-beta1 gene expression and induction of hypertrophy of rat kidney proximal tubular cells in high glucose. J Am Soc Nephrol. 2002;13(2):302-12.
Zhang, S. L., To, C., Chen, X., Filep, J. G., Tang, S. S., Ingelfinger, J. R., & Chan, J. S. (2002). Essential role(s) of the intrarenal renin-angiotensin system in transforming growth factor-beta1 gene expression and induction of hypertrophy of rat kidney proximal tubular cells in high glucose. Journal of the American Society of Nephrology : JASN, 13(2), 302-12.
Zhang SL, et al. Essential Role(s) of the Intrarenal Renin-angiotensin System in Transforming Growth Factor-beta1 Gene Expression and Induction of Hypertrophy of Rat Kidney Proximal Tubular Cells in High Glucose. J Am Soc Nephrol. 2002;13(2):302-12. PubMed PMID: 11805157.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Essential role(s) of the intrarenal renin-angiotensin system in transforming growth factor-beta1 gene expression and induction of hypertrophy of rat kidney proximal tubular cells in high glucose. AU - Zhang,Shao-Ling, AU - To,Catherine, AU - Chen,Xing, AU - Filep,Janos G, AU - Tang,Shiow-Shih, AU - Ingelfinger,Julie R, AU - Chan,John S D, PY - 2002/1/24/pubmed PY - 2002/3/14/medline PY - 2002/1/24/entrez SP - 302 EP - 12 JF - Journal of the American Society of Nephrology : JASN JO - J. Am. Soc. Nephrol. VL - 13 IS - 2 N2 - These studies investigated the question of whether the intrarenal renin-angiotensin system (RAS) is essential for transforming growth factor-beta1 (TGF-beta1) gene expression and induction of hypertrophy of renal proximal tubular cells in high glucose in vitro. Antisense and sense angiotensinogen (ANG) cDNAs were stably transfected into rat immortalized renal proximal tubular cells (IRPTC). ANG secretion from rat IRPTC was quantified by a specific RIA for rat ANG. Cellular ANG, TGF-beta1, and collagen alpha1 (type IV) mRNA levels were determined by Northern blot analysis or by reverse transcriptase-PCR assay. Hypertrophy of IRPTC was analyzed by Western blotting of cellular p27(Kip1) protein, flow cytometry, and cellular protein assay. The results showed that stable transfer of antisense ANG cDNA into IRPTC suppressed the basal TGF-beta1 and collagen alpha1 (type IV) mRNA expression and blocked the stimulatory effect of high glucose (i.e., 25 mM) on TGF-beta1 and collagen alpha1 (type IV) mRNA expression and induction of IRPTC hypertrophy. In contrast, stable transfer of sense ANG cDNA into IRPTC had no significant effect on these parameters. These data demonstrate that local intrarenal RAS activation is essential for TGF-beta1 gene expression and induction of hypertrophy of renal proximal tubular cells in high glucose. SN - 1046-6673 UR - https://www.unboundmedicine.com/medline/citation/11805157/Essential_role_s__of_the_intrarenal_renin_angiotensin_system_in_transforming_growth_factor_beta1_gene_expression_and_induction_of_hypertrophy_of_rat_kidney_proximal_tubular_cells_in_high_glucose_ L2 - http://jasn.asnjournals.org/cgi/pmidlookup?view=long&pmid=11805157 DB - PRIME DP - Unbound Medicine ER -