Tags

Type your tag names separated by a space and hit enter

Subretinally transplanted embryonic stem cells rescue photoreceptor cells from degeneration in the RCS rats.
Cell Transplant. 2001; 10(8):673-80.CT

Abstract

The Royal College of Surgeons (RCS) rat is an animal model for retinal degeneration such as the age-related macular degeneration. The RCS rat undergoes a progressive retinal degeneration during the early postnatal period. A potential treatment to prevent this retinal degeneration is the transplantation into the subretinal space of cells that would replace functions of the degenerating retinal pigment epithelium (RPE) cells or may form neurotrophic factors. In this study we have investigated the potential of subretinally transplanted embryonic stem cells to prevent the genetically determined photoreceptor cell degeneration in the RCS rat. Embryonic stem cells from the inner cell mass of the mouse blastocyst were allowed to differentiate to neural precursor cells in vitro and were then transplanted into the subretinal space of 20-day-old RCS rats. Transplanted and sham-operated rats were sacrificed 2 months following cell transplantation. The eyes were enucleated and photoreceptor degeneration was quantified by analyzing and determining the thickness of the outer nuclear layer by light and electron microscopy. In the eyes transplanted with embryonic cells up to 8 rows of photoreceptor cell nuclei were observed, whereas in nontreated control eyes the outer nuclear layer had degenerated completely. Transplantation of embryonic stem cells appears to delay photoreceptor cell degeneration in RCS rats.

Authors+Show Affiliations

Department of Vitreoretinal Surgery, University of Cologne, Germany. U.Schraemeyer@uni-koeln.deNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Comparative Study
Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

11814109

Citation

Schraermeyer, U, et al. "Subretinally Transplanted Embryonic Stem Cells Rescue Photoreceptor Cells From Degeneration in the RCS Rats." Cell Transplantation, vol. 10, no. 8, 2001, pp. 673-80.
Schraermeyer U, Thumann G, Luther T, et al. Subretinally transplanted embryonic stem cells rescue photoreceptor cells from degeneration in the RCS rats. Cell Transplant. 2001;10(8):673-80.
Schraermeyer, U., Thumann, G., Luther, T., Kociok, N., Armhold, S., Kruttwig, K., Andressen, C., Addicks, K., & Bartz-Schmidt, K. U. (2001). Subretinally transplanted embryonic stem cells rescue photoreceptor cells from degeneration in the RCS rats. Cell Transplantation, 10(8), 673-80.
Schraermeyer U, et al. Subretinally Transplanted Embryonic Stem Cells Rescue Photoreceptor Cells From Degeneration in the RCS Rats. Cell Transplant. 2001;10(8):673-80. PubMed PMID: 11814109.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Subretinally transplanted embryonic stem cells rescue photoreceptor cells from degeneration in the RCS rats. AU - Schraermeyer,U, AU - Thumann,G, AU - Luther,T, AU - Kociok,N, AU - Armhold,S, AU - Kruttwig,K, AU - Andressen,C, AU - Addicks,K, AU - Bartz-Schmidt,K U, PY - 2002/1/30/pubmed PY - 2002/9/6/medline PY - 2002/1/30/entrez SP - 673 EP - 80 JF - Cell transplantation JO - Cell Transplant VL - 10 IS - 8 N2 - The Royal College of Surgeons (RCS) rat is an animal model for retinal degeneration such as the age-related macular degeneration. The RCS rat undergoes a progressive retinal degeneration during the early postnatal period. A potential treatment to prevent this retinal degeneration is the transplantation into the subretinal space of cells that would replace functions of the degenerating retinal pigment epithelium (RPE) cells or may form neurotrophic factors. In this study we have investigated the potential of subretinally transplanted embryonic stem cells to prevent the genetically determined photoreceptor cell degeneration in the RCS rat. Embryonic stem cells from the inner cell mass of the mouse blastocyst were allowed to differentiate to neural precursor cells in vitro and were then transplanted into the subretinal space of 20-day-old RCS rats. Transplanted and sham-operated rats were sacrificed 2 months following cell transplantation. The eyes were enucleated and photoreceptor degeneration was quantified by analyzing and determining the thickness of the outer nuclear layer by light and electron microscopy. In the eyes transplanted with embryonic cells up to 8 rows of photoreceptor cell nuclei were observed, whereas in nontreated control eyes the outer nuclear layer had degenerated completely. Transplantation of embryonic stem cells appears to delay photoreceptor cell degeneration in RCS rats. SN - 0963-6897 UR - https://www.unboundmedicine.com/medline/citation/11814109/Subretinally_transplanted_embryonic_stem_cells_rescue_photoreceptor_cells_from_degeneration_in_the_RCS_rats_ L2 - https://www.lens.org/lens/search/patent/list?q=citation_id:11814109 DB - PRIME DP - Unbound Medicine ER -