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Clastogenicity of pentachlorophenol, 2,4-D and butachlor evaluated by Allium root tip test.
Mutat Res. 2002 Feb 15; 514(1-2):105-13.MR

Abstract

The meristematic mitotic cells of Allium cepa is an efficient cytogenetic material for chromosome aberration assay on environmental pollutants. For assessing genotoxicity of pentachlorophenol (PCP), 2,4-dichlorophenoxyacetic acid (2,4-D) and 2-chloro-2,6-diethyl-N-(butoxymethyl) acetanilide (butachlor), 50% effective concentration (EC(50)), c-mitosis, stickiness, chromosome breaks and mitotic index (MI) were used as endpoints of genotoxicity. EC(50) values for PCP and butachlor are 0.73 and 5.13 ppm, respectively. 2,4-D evidently induced morphological changes at higher concentrations. Some changes like crochet hooks, c-tumours and broken roots were unique to 2,4-D at 5-20 ppm. No such abnormalities were found in PCP and butachlor treated groups, however, root deteriorated and degenerated at higher concentrations (<3 ppm) in PCP. MI in 2,4-D showed a low average of 14.32% followed by PCP (19.53%), while in butachlor it was recorded 71.6%, which is near to the control value. All chemicals induced chromosome aberrations at statistically significant level. The highest chromosome aberration frequency (11.90%) was recorded in PCP at 3 ppm. Large number of c-mitotic anaphases indicated that butachlor acts as potent spindle inhibitor, whereas, breaks, bridges, stickiness and laggards were most frequently found in PCP showing that it is a potent clastogen.

Authors+Show Affiliations

Gene-Tox Laboratory, Division of Genetics, Department of Zoology, Aligarh Muslim University, 202002, UP, Aligarh, India.No affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

11815249

Citation

Ateeq, Bushra, et al. "Clastogenicity of Pentachlorophenol, 2,4-D and Butachlor Evaluated By Allium Root Tip Test." Mutation Research, vol. 514, no. 1-2, 2002, pp. 105-13.
Ateeq B, Abul Farah M, Niamat Ali M, et al. Clastogenicity of pentachlorophenol, 2,4-D and butachlor evaluated by Allium root tip test. Mutat Res. 2002;514(1-2):105-13.
Ateeq, B., Abul Farah, M., Niamat Ali, M., & Ahmad, W. (2002). Clastogenicity of pentachlorophenol, 2,4-D and butachlor evaluated by Allium root tip test. Mutation Research, 514(1-2), 105-13.
Ateeq B, et al. Clastogenicity of Pentachlorophenol, 2,4-D and Butachlor Evaluated By Allium Root Tip Test. Mutat Res. 2002 Feb 15;514(1-2):105-13. PubMed PMID: 11815249.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Clastogenicity of pentachlorophenol, 2,4-D and butachlor evaluated by Allium root tip test. AU - Ateeq,Bushra, AU - Abul Farah,M, AU - Niamat Ali,M, AU - Ahmad,Waseem, PY - 2002/1/30/pubmed PY - 2002/3/29/medline PY - 2002/1/30/entrez SP - 105 EP - 13 JF - Mutation research JO - Mutat. Res. VL - 514 IS - 1-2 N2 - The meristematic mitotic cells of Allium cepa is an efficient cytogenetic material for chromosome aberration assay on environmental pollutants. For assessing genotoxicity of pentachlorophenol (PCP), 2,4-dichlorophenoxyacetic acid (2,4-D) and 2-chloro-2,6-diethyl-N-(butoxymethyl) acetanilide (butachlor), 50% effective concentration (EC(50)), c-mitosis, stickiness, chromosome breaks and mitotic index (MI) were used as endpoints of genotoxicity. EC(50) values for PCP and butachlor are 0.73 and 5.13 ppm, respectively. 2,4-D evidently induced morphological changes at higher concentrations. Some changes like crochet hooks, c-tumours and broken roots were unique to 2,4-D at 5-20 ppm. No such abnormalities were found in PCP and butachlor treated groups, however, root deteriorated and degenerated at higher concentrations (<3 ppm) in PCP. MI in 2,4-D showed a low average of 14.32% followed by PCP (19.53%), while in butachlor it was recorded 71.6%, which is near to the control value. All chemicals induced chromosome aberrations at statistically significant level. The highest chromosome aberration frequency (11.90%) was recorded in PCP at 3 ppm. Large number of c-mitotic anaphases indicated that butachlor acts as potent spindle inhibitor, whereas, breaks, bridges, stickiness and laggards were most frequently found in PCP showing that it is a potent clastogen. SN - 0027-5107 UR - https://www.unboundmedicine.com/medline/citation/11815249/Clastogenicity_of_pentachlorophenol_24_D_and_butachlor_evaluated_by_Allium_root_tip_test_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S1383571801003278 DB - PRIME DP - Unbound Medicine ER -