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Identification of myosin light chains in Rana pipiens skeletal muscle and their expression patterns along single fibres.
J Exp Biol. 2001 Dec; 204(Pt 24):4237-48.JE

Abstract

Isoforms of myosin heavy chain (MHC) and myosin light chain (MLC) influence contractile kinetics of skeletal muscle. We previously showed that the four major skeletal muscle fibre types in Rana pipiens (type 1, type 2, type 3 and tonic; amphibian nomenclature) contain four unique MHC isoforms. In the present study we defined the MLCs expressed in each of these R. pipiens fibre types. The MLC composition of single MHC-typed fibres was determined from western blots using a panel of monoclonal MLC antibodies. A total of seven MLCs were identified, including four types of MLC1, two of MLC2 and a single MLC3. Twitch fibre types (types 1, 2 and 3) expressed MLC1(f) and MLC2(f), while tonic fibres contained a unique set of isoforms, MLC1(Ta), MLC1(Tb) and MLC2(T). MLC3 was expressed primarily in type 1, type 1-2 and type 2 fibres. Surprisingly, some frogs displayed a striking pattern of MLC expression where a unique isoform of MLC1 (MLC1(x)) was coexpressed along with the normal MLC1 isoform(s) in all fibre types. MLC1(x) was either expressed in all fibres of a given frog or was completely absent. The intraspecific polymorphism in MLC1 expression is likely to have a genetic basis, but is unlikely to be caused by allelic variation. The ratio of MLC3/MLC1 increased in direct proportion to the percentage of type 1 MHC, but was only weakly correlated. The variability in MLC3/MLC1 within a fibre type was extremely large. Both the MHC isoform and MLC3/MLC1 ratio varied significantly between 1 mm segments along the length of fibres. For all segments combined, MLC3/MLC1 increased with the percentage of type 1 MHC, but the correlation between segments was weaker than between fibres.

Authors+Show Affiliations

Department of Orthopaedics, University of California San Diego School of Medicine, Veterans Affairs Medical Center, 3350 La Jolla Village Drive, San Diego, CA 92161, USA. glutz@ucsd.eduNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, U.S. Gov't, Non-P.H.S.
Research Support, U.S. Gov't, P.H.S.

Language

eng

PubMed ID

11815648

Citation

Lutz, G J., et al. "Identification of Myosin Light Chains in Rana Pipiens Skeletal Muscle and Their Expression Patterns Along Single Fibres." The Journal of Experimental Biology, vol. 204, no. Pt 24, 2001, pp. 4237-48.
Lutz GJ, Bremner SN, Bade MJ, et al. Identification of myosin light chains in Rana pipiens skeletal muscle and their expression patterns along single fibres. J Exp Biol. 2001;204(Pt 24):4237-48.
Lutz, G. J., Bremner, S. N., Bade, M. J., & Lieber, R. L. (2001). Identification of myosin light chains in Rana pipiens skeletal muscle and their expression patterns along single fibres. The Journal of Experimental Biology, 204(Pt 24), 4237-48.
Lutz GJ, et al. Identification of Myosin Light Chains in Rana Pipiens Skeletal Muscle and Their Expression Patterns Along Single Fibres. J Exp Biol. 2001;204(Pt 24):4237-48. PubMed PMID: 11815648.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Identification of myosin light chains in Rana pipiens skeletal muscle and their expression patterns along single fibres. AU - Lutz,G J, AU - Bremner,S N, AU - Bade,M J, AU - Lieber,R L, PY - 2002/1/30/pubmed PY - 2002/4/18/medline PY - 2002/1/30/entrez SP - 4237 EP - 48 JF - The Journal of experimental biology JO - J Exp Biol VL - 204 IS - Pt 24 N2 - Isoforms of myosin heavy chain (MHC) and myosin light chain (MLC) influence contractile kinetics of skeletal muscle. We previously showed that the four major skeletal muscle fibre types in Rana pipiens (type 1, type 2, type 3 and tonic; amphibian nomenclature) contain four unique MHC isoforms. In the present study we defined the MLCs expressed in each of these R. pipiens fibre types. The MLC composition of single MHC-typed fibres was determined from western blots using a panel of monoclonal MLC antibodies. A total of seven MLCs were identified, including four types of MLC1, two of MLC2 and a single MLC3. Twitch fibre types (types 1, 2 and 3) expressed MLC1(f) and MLC2(f), while tonic fibres contained a unique set of isoforms, MLC1(Ta), MLC1(Tb) and MLC2(T). MLC3 was expressed primarily in type 1, type 1-2 and type 2 fibres. Surprisingly, some frogs displayed a striking pattern of MLC expression where a unique isoform of MLC1 (MLC1(x)) was coexpressed along with the normal MLC1 isoform(s) in all fibre types. MLC1(x) was either expressed in all fibres of a given frog or was completely absent. The intraspecific polymorphism in MLC1 expression is likely to have a genetic basis, but is unlikely to be caused by allelic variation. The ratio of MLC3/MLC1 increased in direct proportion to the percentage of type 1 MHC, but was only weakly correlated. The variability in MLC3/MLC1 within a fibre type was extremely large. Both the MHC isoform and MLC3/MLC1 ratio varied significantly between 1 mm segments along the length of fibres. For all segments combined, MLC3/MLC1 increased with the percentage of type 1 MHC, but the correlation between segments was weaker than between fibres. SN - 0022-0949 UR - https://www.unboundmedicine.com/medline/citation/11815648/Identification_of_myosin_light_chains_in_Rana_pipiens_skeletal_muscle_and_their_expression_patterns_along_single_fibres_ L2 - http://jeb.biologists.org/cgi/pmidlookup?view=long&pmid=11815648 DB - PRIME DP - Unbound Medicine ER -