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The adaptive response of Saccharomyces cerevisiae to mercury exposure.
Yeast. 2002 Feb; 19(3):233-9.Y

Abstract

The budding yeast Saccharomyces cerevisiae has been shown to possess a number of discrete but overlapping adaptive stress responses. We show here that yeast has an adaptive stress response towards mercury and that this response overlaps to some extent with the H(2)O(2) and cadmium-inducible stress responses. Expression of the yeast GSH1 gene, encoding gamma-glutamylcysteine synthetase, is known to be regulated by hydrogen peroxide; in this study we show that expression of a GSH1-lacZ reporter gene is shown to be regulated by exposure to heavy metals, such as mercury and cadmium. Other redox-active metals, including copper and iron, were found not to induce GSH1 expression. We show that mercury-mediated regulation of the GSH1 gene is not by the same mechanism used by cadmium. Moreover, our experiments suggest the possibility that the oxidative stress produced by mercury exposure is similar to that produced by treatment with H(2)O(2), consistent with our finding that the Yap1 protein is also involved in the response of yeast towards mercury.

Authors+Show Affiliations

Westwater J
Department of Biological Sciences, Heriot-Watt University, Edinburgh EH14 4AS, UK.
McLaren NF
No affiliation info available
Dormer UH
No affiliation info available
Jamieson DJ
No affiliation info available

MeSH

Adaptation, BiologicalCadmium ChlorideGene Expression Regulation, FungalGlutathioneHydrogen PeroxideMercuric ChlorideOxidantsOxidative StressSaccharomyces cerevisiaeVitamin K 3

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

11816031

Citation

Westwater, John, et al. "The Adaptive Response of Saccharomyces Cerevisiae to Mercury Exposure." Yeast (Chichester, England), vol. 19, no. 3, 2002, pp. 233-9.
Westwater J, McLaren NF, Dormer UH, et al. The adaptive response of Saccharomyces cerevisiae to mercury exposure. Yeast. 2002;19(3):233-9.
Westwater, J., McLaren, N. F., Dormer, U. H., & Jamieson, D. J. (2002). The adaptive response of Saccharomyces cerevisiae to mercury exposure. Yeast (Chichester, England), 19(3), 233-9.
Westwater J, et al. The Adaptive Response of Saccharomyces Cerevisiae to Mercury Exposure. Yeast. 2002;19(3):233-9. PubMed PMID: 11816031.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - The adaptive response of Saccharomyces cerevisiae to mercury exposure. AU - Westwater,John, AU - McLaren,Niall F, AU - Dormer,Ulla H, AU - Jamieson,Derek J, PY - 2002/1/30/pubmed PY - 2002/4/25/medline PY - 2002/1/30/entrez SP - 233 EP - 9 JF - Yeast (Chichester, England) JO - Yeast VL - 19 IS - 3 N2 - The budding yeast Saccharomyces cerevisiae has been shown to possess a number of discrete but overlapping adaptive stress responses. We show here that yeast has an adaptive stress response towards mercury and that this response overlaps to some extent with the H(2)O(2) and cadmium-inducible stress responses. Expression of the yeast GSH1 gene, encoding gamma-glutamylcysteine synthetase, is known to be regulated by hydrogen peroxide; in this study we show that expression of a GSH1-lacZ reporter gene is shown to be regulated by exposure to heavy metals, such as mercury and cadmium. Other redox-active metals, including copper and iron, were found not to induce GSH1 expression. We show that mercury-mediated regulation of the GSH1 gene is not by the same mechanism used by cadmium. Moreover, our experiments suggest the possibility that the oxidative stress produced by mercury exposure is similar to that produced by treatment with H(2)O(2), consistent with our finding that the Yap1 protein is also involved in the response of yeast towards mercury. SN - 0749-503X UR - https://www.unboundmedicine.com/medline/citation/11816031/The_adaptive_response_of_Saccharomyces_cerevisiae_to_mercury_exposure_ L2 - https://doi.org/10.1002/yea.835 DB - PRIME DP - Unbound Medicine ER -