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An analysis of inter- and intraspecific genetic variabilities in the Kluyveromyces marxianus group of yeast species for the reconsideration of the K. lactis taxon.
Yeast. 2002 Feb; 19(3):257-68.Y

Abstract

In the present work, we analyse the sequences of the 5.8S rRNA gene and the two internal transcribed spacers 1 and 2 (5.8S-ITS region), obtained from 39 strains belonging to the species Kluyveromyces aestuarii, K. dobzhanskii, K. lactis and K. marxianus, K. nonfermentans and K. wickerhamii, to solve the phylogenetic relationships among these species and also to determine the possible genetic basis for the delimitation of the two currently accepted K. lactis varieties: lactis, including lactose-positive strains isolated from dairy products, and drosophilarum, comprising lactose-negative strains isolated from insects and plant exudates. The determination of the phylogenetic relationships within the species K. lactis, together with the examination of the electrophoretic karyotypes and phenotypic characterization of strains representatives of K. lactis var. lactis and var. drosophilarum, allowed differentiation of two groups of strains. The first, and ancestral, group comprises lactose-negative strains isolated from natural habitats in North America. The second, and derived, group includes both lactose-negative strains isolated from natural habitats in Europe and wine fermentation in South Africa, and lactose-positive strains associated with dairy products. These results suggest that the present taxon K. lactis is a complex of different species, subspecies or, at least, genetically structured populations.

Authors+Show Affiliations

Belloch C
Colección Española de Cultivos Tipo (CECT), Universitat de Valencia, Calle Dr Moliner 50, 46100 Burjassot, Valencia, Spain.
Fernández-Espinar T
No affiliation info available
Querol A
No affiliation info available
Dolores García M
No affiliation info available
Barrio E
No affiliation info available

MeSH

Base SequenceDNA, FungalDNA, Ribosomal SpacerElectrophoresis, Gel, Pulsed-FieldEuropeFood MicrobiologyGenetic VariationKluyveromycesMolecular Sequence DataNorth AmericaPhylogenyPolymerase Chain ReactionPolymorphism, GeneticRNA, Ribosomal, 5.8SSequence AlignmentSequence Analysis, DNASequence Homology, Nucleic AcidSouth Africa

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

11816033

Citation

Belloch, Carmela, et al. "An Analysis of Inter- and Intraspecific Genetic Variabilities in the Kluyveromyces Marxianus Group of Yeast Species for the Reconsideration of the K. Lactis Taxon." Yeast (Chichester, England), vol. 19, no. 3, 2002, pp. 257-68.
Belloch C, Fernández-Espinar T, Querol A, et al. An analysis of inter- and intraspecific genetic variabilities in the Kluyveromyces marxianus group of yeast species for the reconsideration of the K. lactis taxon. Yeast. 2002;19(3):257-68.
Belloch, C., Fernández-Espinar, T., Querol, A., Dolores García, M., & Barrio, E. (2002). An analysis of inter- and intraspecific genetic variabilities in the Kluyveromyces marxianus group of yeast species for the reconsideration of the K. lactis taxon. Yeast (Chichester, England), 19(3), 257-68.
Belloch C, et al. An Analysis of Inter- and Intraspecific Genetic Variabilities in the Kluyveromyces Marxianus Group of Yeast Species for the Reconsideration of the K. Lactis Taxon. Yeast. 2002;19(3):257-68. PubMed PMID: 11816033.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - An analysis of inter- and intraspecific genetic variabilities in the Kluyveromyces marxianus group of yeast species for the reconsideration of the K. lactis taxon. AU - Belloch,Carmela, AU - Fernández-Espinar,Teresa, AU - Querol,Amparo, AU - Dolores García,M, AU - Barrio,Eladio, PY - 2002/1/30/pubmed PY - 2002/4/25/medline PY - 2002/1/30/entrez SP - 257 EP - 68 JF - Yeast (Chichester, England) JO - Yeast VL - 19 IS - 3 N2 - In the present work, we analyse the sequences of the 5.8S rRNA gene and the two internal transcribed spacers 1 and 2 (5.8S-ITS region), obtained from 39 strains belonging to the species Kluyveromyces aestuarii, K. dobzhanskii, K. lactis and K. marxianus, K. nonfermentans and K. wickerhamii, to solve the phylogenetic relationships among these species and also to determine the possible genetic basis for the delimitation of the two currently accepted K. lactis varieties: lactis, including lactose-positive strains isolated from dairy products, and drosophilarum, comprising lactose-negative strains isolated from insects and plant exudates. The determination of the phylogenetic relationships within the species K. lactis, together with the examination of the electrophoretic karyotypes and phenotypic characterization of strains representatives of K. lactis var. lactis and var. drosophilarum, allowed differentiation of two groups of strains. The first, and ancestral, group comprises lactose-negative strains isolated from natural habitats in North America. The second, and derived, group includes both lactose-negative strains isolated from natural habitats in Europe and wine fermentation in South Africa, and lactose-positive strains associated with dairy products. These results suggest that the present taxon K. lactis is a complex of different species, subspecies or, at least, genetically structured populations. SN - 0749-503X UR - https://www.unboundmedicine.com/medline/citation/11816033/An_analysis_of_inter__and_intraspecific_genetic_variabilities_in_the_Kluyveromyces_marxianus_group_of_yeast_species_for_the_reconsideration_of_the_K__lactis_taxon_ L2 - https://doi.org/10.1002/yea.832 DB - PRIME DP - Unbound Medicine ER -