Tags

Type your tag names separated by a space and hit enter

Isoprenoid biosynthesis via 1-deoxy-D-xylulose 5-phosphate/2-C-methyl-D-erythritol 4-phosphate (DOXP/MEP) pathway.
Acta Biochim Pol. 2001; 48(3):663-72.AB

Abstract

Higher plants, several algae, bacteria, some strains of Streptomyces and possibly malaria parasite Plasmodium falciparum contain the novel, plastidic DOXP/MEP pathway for isoprenoid biosynthesis. This pathway, alternative with respect to the classical mevalonate pathway, starts with condensation of pyruvate and glyceraldehyde-3-phosphate which yields 1-deoxy-D-xylulose 5-phosphate (DOXP); the latter product can be converted to isopentenyl diphosphate (IPP) and eventually to isoprenoids or thiamine and pyridoxal. Subsequent reactions of this pathway involve transformation of DOXP to 2-C-methyl-D-erythritol 4-phosphate (MEP) which after condensation with CTP forms 4-diphosphocytidyl-2-amethyl-D-erythritol (CDP-ME). Then CDP-ME is phosphorylated to 4-diphosphocytidyl-2-amethyl-D-erythritol 2-phosphate (CDP-ME2P) and to 2-C-methyl-D-erythritol-2,4-cyclodiphosphate (ME-2,4cPP) which is the last known intermediate of the DOXP/MEP pathway. For- mation of IPP and dimethylallyl diphosphate (DMAPP) from ME-2,4cPP still requires clarification. This novel pathway appears to be involved in biosynthesis of carotenoids, phytol (side chain of chlorophylls), isoprene, mono-, di-, tetraterpenes and plastoquinone whereas the mevalonate pathway is responsible for formation of sterols, sesquiterpenes and triterpenes. Several isoprenoids were found to be of mixed origin suggesting that some exchange and/or cooperation exists between these two pathways of different biosynthetic origin. Contradictory results described below could indicate that these two pathways are operating under different physiological conditions of the cell and are dependent on the developmental state of plastids.

Links

FREE Publisher Full Text

Authors+Show Affiliations

Wanke M
Institute of Biochemistry and Biophysics, Polish Academy of Sciences, Warszawa. malgorzataw@ibb.waw.pl
Skorupinska-Tudek K
No affiliation info available
Swiezewska E
No affiliation info available

MeSH

AnimalsEndocytosisErythritolHemiterpenesOrganophosphorus CompoundsPentosephosphatesPlantsSugar PhosphatesSymbiosis

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't
Review

Language

eng

PubMed ID

11833775

Citation

Wanke, M, et al. "Isoprenoid Biosynthesis Via 1-deoxy-D-xylulose 5-phosphate/2-C-methyl-D-erythritol 4-phosphate (DOXP/MEP) Pathway." Acta Biochimica Polonica, vol. 48, no. 3, 2001, pp. 663-72.
Wanke M, Skorupinska-Tudek K, Swiezewska E. Isoprenoid biosynthesis via 1-deoxy-D-xylulose 5-phosphate/2-C-methyl-D-erythritol 4-phosphate (DOXP/MEP) pathway. Acta Biochim Pol. 2001;48(3):663-72.
Wanke, M., Skorupinska-Tudek, K., & Swiezewska, E. (2001). Isoprenoid biosynthesis via 1-deoxy-D-xylulose 5-phosphate/2-C-methyl-D-erythritol 4-phosphate (DOXP/MEP) pathway. Acta Biochimica Polonica, 48(3), 663-72.
Wanke M, Skorupinska-Tudek K, Swiezewska E. Isoprenoid Biosynthesis Via 1-deoxy-D-xylulose 5-phosphate/2-C-methyl-D-erythritol 4-phosphate (DOXP/MEP) Pathway. Acta Biochim Pol. 2001;48(3):663-72. PubMed PMID: 11833775.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Isoprenoid biosynthesis via 1-deoxy-D-xylulose 5-phosphate/2-C-methyl-D-erythritol 4-phosphate (DOXP/MEP) pathway. AU - Wanke,M, AU - Skorupinska-Tudek,K, AU - Swiezewska,E, PY - 2002/2/9/pubmed PY - 2002/8/16/medline PY - 2002/2/9/entrez SP - 663 EP - 72 JF - Acta biochimica Polonica JO - Acta Biochim Pol VL - 48 IS - 3 N2 - Higher plants, several algae, bacteria, some strains of Streptomyces and possibly malaria parasite Plasmodium falciparum contain the novel, plastidic DOXP/MEP pathway for isoprenoid biosynthesis. This pathway, alternative with respect to the classical mevalonate pathway, starts with condensation of pyruvate and glyceraldehyde-3-phosphate which yields 1-deoxy-D-xylulose 5-phosphate (DOXP); the latter product can be converted to isopentenyl diphosphate (IPP) and eventually to isoprenoids or thiamine and pyridoxal. Subsequent reactions of this pathway involve transformation of DOXP to 2-C-methyl-D-erythritol 4-phosphate (MEP) which after condensation with CTP forms 4-diphosphocytidyl-2-amethyl-D-erythritol (CDP-ME). Then CDP-ME is phosphorylated to 4-diphosphocytidyl-2-amethyl-D-erythritol 2-phosphate (CDP-ME2P) and to 2-C-methyl-D-erythritol-2,4-cyclodiphosphate (ME-2,4cPP) which is the last known intermediate of the DOXP/MEP pathway. For- mation of IPP and dimethylallyl diphosphate (DMAPP) from ME-2,4cPP still requires clarification. This novel pathway appears to be involved in biosynthesis of carotenoids, phytol (side chain of chlorophylls), isoprene, mono-, di-, tetraterpenes and plastoquinone whereas the mevalonate pathway is responsible for formation of sterols, sesquiterpenes and triterpenes. Several isoprenoids were found to be of mixed origin suggesting that some exchange and/or cooperation exists between these two pathways of different biosynthetic origin. Contradictory results described below could indicate that these two pathways are operating under different physiological conditions of the cell and are dependent on the developmental state of plastids. SN - 0001-527X UR - https://www.unboundmedicine.com/medline/citation/11833775/Isoprenoid_biosynthesis_via_1_deoxy_D_xylulose_5_phosphate/2_C_methyl_D_erythritol_4_phosphate__DOXP/MEP__pathway_ L2 - http://www.actabp.pl/pdf/3_2001/663.pdf DB - PRIME DP - Unbound Medicine ER -