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Macrophage migration inhibitory factor is markedly expressed in active and early-stage endometriotic lesions.
J Clin Endocrinol Metab 2002; 87(2):883-9JC

Abstract

The establishment of a new vascular supply is essential for the survival of endometrial tissue and its development in ectopic locations. We have previously shown that ectopic endometrial cells release an important mitogenic activity for human endothelial cells and identified macrophage migration inhibitory factor (MIF) as one of the principal bioactive molecules involved in endothelial cell proliferation. In the present study, immunohistochemical and dual immunofluorescence analyses showed that MIF is effectively expressed by endometriotic tissue, particularly in the glands, and identified endothelial cells, macrophages, and T lymphocytes as cells markedly expressing MIF in the stroma. Western blot analysis showed a single 12.5-kDa band corresponding to the known mol wt of the molecule. The highest concentrations of MIF protein in endometriotic tissue, as measured by ELISA, were found in flame-like red endometriotic lesions, compared with typical black-bluish (P < 0.01) or with white lesions (P < 0.01). Interestingly, MIF displayed a marked expression in lesions from the initial stage of endometriosis (stage I). Semiquantitative RT-PCR analysis of MIF mRNA levels in the same endometriotic tissues showed a pattern of expression comparable with that of the protein. In view of its potent proinflammatory and angiogenic properties, local production of MIF within endometrial implants, particularly in those that are highly vascularized and representing the earliest and most active forms of the disease, make plausible the involvement of this factor in the local immunoinflammatory process observed in endometriosis and the initial steps of endometriotic tissue growth and development.

Authors+Show Affiliations

Unité d'Endocrinologie de la Reproduction, Centre de Recherche, Hôpital Saint-François d'Assise, Centre Hospitalier Universitaire de Québec, Université Laval, Québec, Canada G1L 3L5.No affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

11836337

Citation

Kats, Rouslan, et al. "Macrophage Migration Inhibitory Factor Is Markedly Expressed in Active and Early-stage Endometriotic Lesions." The Journal of Clinical Endocrinology and Metabolism, vol. 87, no. 2, 2002, pp. 883-9.
Kats R, Metz CN, Akoum A. Macrophage migration inhibitory factor is markedly expressed in active and early-stage endometriotic lesions. J Clin Endocrinol Metab. 2002;87(2):883-9.
Kats, R., Metz, C. N., & Akoum, A. (2002). Macrophage migration inhibitory factor is markedly expressed in active and early-stage endometriotic lesions. The Journal of Clinical Endocrinology and Metabolism, 87(2), pp. 883-9.
Kats R, Metz CN, Akoum A. Macrophage Migration Inhibitory Factor Is Markedly Expressed in Active and Early-stage Endometriotic Lesions. J Clin Endocrinol Metab. 2002;87(2):883-9. PubMed PMID: 11836337.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Macrophage migration inhibitory factor is markedly expressed in active and early-stage endometriotic lesions. AU - Kats,Rouslan, AU - Metz,Christine N, AU - Akoum,Ali, PY - 2002/2/12/pubmed PY - 2002/3/2/medline PY - 2002/2/12/entrez SP - 883 EP - 9 JF - The Journal of clinical endocrinology and metabolism JO - J. Clin. Endocrinol. Metab. VL - 87 IS - 2 N2 - The establishment of a new vascular supply is essential for the survival of endometrial tissue and its development in ectopic locations. We have previously shown that ectopic endometrial cells release an important mitogenic activity for human endothelial cells and identified macrophage migration inhibitory factor (MIF) as one of the principal bioactive molecules involved in endothelial cell proliferation. In the present study, immunohistochemical and dual immunofluorescence analyses showed that MIF is effectively expressed by endometriotic tissue, particularly in the glands, and identified endothelial cells, macrophages, and T lymphocytes as cells markedly expressing MIF in the stroma. Western blot analysis showed a single 12.5-kDa band corresponding to the known mol wt of the molecule. The highest concentrations of MIF protein in endometriotic tissue, as measured by ELISA, were found in flame-like red endometriotic lesions, compared with typical black-bluish (P < 0.01) or with white lesions (P < 0.01). Interestingly, MIF displayed a marked expression in lesions from the initial stage of endometriosis (stage I). Semiquantitative RT-PCR analysis of MIF mRNA levels in the same endometriotic tissues showed a pattern of expression comparable with that of the protein. In view of its potent proinflammatory and angiogenic properties, local production of MIF within endometrial implants, particularly in those that are highly vascularized and representing the earliest and most active forms of the disease, make plausible the involvement of this factor in the local immunoinflammatory process observed in endometriosis and the initial steps of endometriotic tissue growth and development. SN - 0021-972X UR - https://www.unboundmedicine.com/medline/citation/11836337/Macrophage_migration_inhibitory_factor_is_markedly_expressed_in_active_and_early_stage_endometriotic_lesions_ L2 - https://academic.oup.com/jcem/article-lookup/doi/10.1210/jcem.87.2.8260 DB - PRIME DP - Unbound Medicine ER -