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Adenosine A(3) receptor-mediated potentiation of mucociliary transport and epithelial ciliary motility.
Am J Physiol Lung Cell Mol Physiol. 2002 Mar; 282(3):L556-62.AJ

Abstract

To examine the effect of adenosine A(3) receptor stimulation on airway mucociliary clearance, we measured transport of Evans blue dye in rabbit trachea in vivo and ciliary motility of epithelium by the photoelectric method in vitro. Mucociliary transport was enhanced dose dependently by the selective A(3) agonist N(6)-(3-iodobenzyl)-5'-N-methylcarbamoyladenosine (IB-MECA) and to a lesser extent by the less-selective N(6)-2-(4-amino-3-iodophenyl)ethyladenosine, whereas the A(1) agonist N-cyclopentyladenosine (CPA) and the A(2) agonist CGS-21680 had no effect. The effect of IB-MECA was abolished by pretreatment with the selective A(3) antagonist MRS-1220 but not by the A(1) antagonist 1,3-dipropyly-8-cyclopentylxanthine or the A(2) antagonist 3,7-dimethyl-L-propargylxanthine. Epithelial ciliary beat frequency was increased by IB-MECA in a concentration-dependent manner, the maximal increase being 33%, and this effect was inhibited by MRS-1220. The IB-MECA-induced ciliary stimulation was not altered by the Rp diastereomer of cAMP but was greatly inhibited by Ca(2+)-free medium containing BAPTA-AM. Incubation with IB-MECA increased intracellular Ca(2+) contents. Therefore, A(3) agonist enhances airway mucociliary clearance probably through Ca(2+)-mediated stimulation of ciliary motility of airway epithelium.

Authors+Show Affiliations

First Department of Medicine, Tokyo Women's Medical University School of Medicine, Tokyo 162-8666, Japan.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

11839552

Citation

Taira, Manako, et al. "Adenosine A(3) Receptor-mediated Potentiation of Mucociliary Transport and Epithelial Ciliary Motility." American Journal of Physiology. Lung Cellular and Molecular Physiology, vol. 282, no. 3, 2002, pp. L556-62.
Taira M, Tamaoki J, Nishimura K, et al. Adenosine A(3) receptor-mediated potentiation of mucociliary transport and epithelial ciliary motility. Am J Physiol Lung Cell Mol Physiol. 2002;282(3):L556-62.
Taira, M., Tamaoki, J., Nishimura, K., Nakata, J., Kondo, M., Takemura, H., & Nagai, A. (2002). Adenosine A(3) receptor-mediated potentiation of mucociliary transport and epithelial ciliary motility. American Journal of Physiology. Lung Cellular and Molecular Physiology, 282(3), L556-62.
Taira M, et al. Adenosine A(3) Receptor-mediated Potentiation of Mucociliary Transport and Epithelial Ciliary Motility. Am J Physiol Lung Cell Mol Physiol. 2002;282(3):L556-62. PubMed PMID: 11839552.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Adenosine A(3) receptor-mediated potentiation of mucociliary transport and epithelial ciliary motility. AU - Taira,Manako, AU - Tamaoki,Jun, AU - Nishimura,Kazuyuki, AU - Nakata,Junko, AU - Kondo,Mitsuko, AU - Takemura,Hisashi, AU - Nagai,Atsushi, PY - 2002/2/13/pubmed PY - 2002/3/8/medline PY - 2002/2/13/entrez SP - L556 EP - 62 JF - American journal of physiology. Lung cellular and molecular physiology JO - Am J Physiol Lung Cell Mol Physiol VL - 282 IS - 3 N2 - To examine the effect of adenosine A(3) receptor stimulation on airway mucociliary clearance, we measured transport of Evans blue dye in rabbit trachea in vivo and ciliary motility of epithelium by the photoelectric method in vitro. Mucociliary transport was enhanced dose dependently by the selective A(3) agonist N(6)-(3-iodobenzyl)-5'-N-methylcarbamoyladenosine (IB-MECA) and to a lesser extent by the less-selective N(6)-2-(4-amino-3-iodophenyl)ethyladenosine, whereas the A(1) agonist N-cyclopentyladenosine (CPA) and the A(2) agonist CGS-21680 had no effect. The effect of IB-MECA was abolished by pretreatment with the selective A(3) antagonist MRS-1220 but not by the A(1) antagonist 1,3-dipropyly-8-cyclopentylxanthine or the A(2) antagonist 3,7-dimethyl-L-propargylxanthine. Epithelial ciliary beat frequency was increased by IB-MECA in a concentration-dependent manner, the maximal increase being 33%, and this effect was inhibited by MRS-1220. The IB-MECA-induced ciliary stimulation was not altered by the Rp diastereomer of cAMP but was greatly inhibited by Ca(2+)-free medium containing BAPTA-AM. Incubation with IB-MECA increased intracellular Ca(2+) contents. Therefore, A(3) agonist enhances airway mucociliary clearance probably through Ca(2+)-mediated stimulation of ciliary motility of airway epithelium. SN - 1040-0605 UR - https://www.unboundmedicine.com/medline/citation/11839552/Adenosine_A_3__receptor_mediated_potentiation_of_mucociliary_transport_and_epithelial_ciliary_motility_ L2 - https://journals.physiology.org/doi/10.1152/ajplung.00360.2001?url_ver=Z39.88-2003&rfr_id=ori:rid:crossref.org&rfr_dat=cr_pub=pubmed DB - PRIME DP - Unbound Medicine ER -