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Analysis of artichoke (Cynara cardunculus L.) extract by means of micellar electrokinetic capillary chromatography.
Electrophoresis. 2002 Jan; 23(2):249-52.E

Abstract

The main constituents of artichoke extract were separated by micellar electrokinetic chromatography (MEKC), using a buffer consisting of 100 mM sodium dodecyl sulfate (SDS) in 20 mM sodium dihydrogen phosphate, 20 mM disodium tetraborate (pH 8.6) as background electrolyte. Optimum separation voltage of 28 kV (positive polarity) and a capillary temperature of 25 degrees C gave the best analysis. The UV detection was performed at 200 nm. The method was successfully used to analyze plant and drug samples as well as for the study of artichoke antioxidant activity. The quantitative MEKC results were in good agreement to those obtained previously by reversed-phase high-performance liquid chromatography (RP-HPLC).

Authors+Show Affiliations

Sevcíková P
Department of Biochemistry, Faculty of Science, Masaryk University, Brno, Czech Republic.
Glatz Z
No affiliation info available
Slanina J
No affiliation info available

MeSH

AsteraceaeChromatography, Micellar Electrokinetic CapillaryMolecular StructurePlant Extracts

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

11840531

Citation

Sevcíková, Petra, et al. "Analysis of Artichoke (Cynara Cardunculus L.) Extract By Means of Micellar Electrokinetic Capillary Chromatography." Electrophoresis, vol. 23, no. 2, 2002, pp. 249-52.
Sevcíková P, Glatz Z, Slanina J. Analysis of artichoke (Cynara cardunculus L.) extract by means of micellar electrokinetic capillary chromatography. Electrophoresis. 2002;23(2):249-52.
Sevcíková, P., Glatz, Z., & Slanina, J. (2002). Analysis of artichoke (Cynara cardunculus L.) extract by means of micellar electrokinetic capillary chromatography. Electrophoresis, 23(2), 249-52.
Sevcíková P, Glatz Z, Slanina J. Analysis of Artichoke (Cynara Cardunculus L.) Extract By Means of Micellar Electrokinetic Capillary Chromatography. Electrophoresis. 2002;23(2):249-52. PubMed PMID: 11840531.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Analysis of artichoke (Cynara cardunculus L.) extract by means of micellar electrokinetic capillary chromatography. AU - Sevcíková,Petra, AU - Glatz,Zdenek, AU - Slanina,Jirí, PY - 2002/2/13/pubmed PY - 2002/5/23/medline PY - 2002/2/13/entrez SP - 249 EP - 52 JF - Electrophoresis JO - Electrophoresis VL - 23 IS - 2 N2 - The main constituents of artichoke extract were separated by micellar electrokinetic chromatography (MEKC), using a buffer consisting of 100 mM sodium dodecyl sulfate (SDS) in 20 mM sodium dihydrogen phosphate, 20 mM disodium tetraborate (pH 8.6) as background electrolyte. Optimum separation voltage of 28 kV (positive polarity) and a capillary temperature of 25 degrees C gave the best analysis. The UV detection was performed at 200 nm. The method was successfully used to analyze plant and drug samples as well as for the study of artichoke antioxidant activity. The quantitative MEKC results were in good agreement to those obtained previously by reversed-phase high-performance liquid chromatography (RP-HPLC). SN - 0173-0835 UR - https://www.unboundmedicine.com/medline/citation/11840531/Analysis_of_artichoke__Cynara_cardunculus_L___extract_by_means_of_micellar_electrokinetic_capillary_chromatography_ L2 - https://doi.org/10.1002/1522-2683(200202)23:2<249::AID-ELPS249>3.0.CO;2-Y DB - PRIME DP - Unbound Medicine ER -