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Determination of lignans in Schisandra chinensis using micellar electrokinetic capillary chromatography.
Electrophoresis. 2002 Jan; 23(2):253-8.E

Abstract

Micellar electrokinetic capillary chromatography (MEKC) has been developed as a promising method for the determination of lignans in plant samples. The separation conditions have been optimized with respect to the different parameters including sodium dodecyl sulfate (SDS) and acetonitrile concentration, pH of the background electrolyte, separation voltage, and capillary temperature. The background electrolyte consisting of 40 mM SDS and 35% acetonitrile in 10 mM tetraborate buffer (pH 9.3) was found to be the most suitable electrolyte for this analysis. The applied voltage of 28 kV (positive polarity) and the capillary temperature 25 degrees C gave the best separation of lignans. The interday reproducibility of the peak areas and the migration times was below 2.0%. The results of MEKC analyses were compared with those obtained by capillary electrochromatography (CEC) and reversed-phase high-performance liquid chromatography (RP-HPLC). The possibilities of using this method for the determination of lignans in drug and in serum samples were also tested.

Authors+Show Affiliations

Sterbová H
Department of Biochemistry, Faculty of Medicine, Masaryk University, Brno, Czech Republic.
Sevcíková P
No affiliation info available
Kvasnicková L
No affiliation info available
Glatz Z
No affiliation info available
Slanina J
No affiliation info available

MeSH

Chromatography, Micellar Electrokinetic CapillaryHumansLignansMolecular StructureSchisandraceae

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

11840532

Citation

Sterbová, Hana, et al. "Determination of Lignans in Schisandra Chinensis Using Micellar Electrokinetic Capillary Chromatography." Electrophoresis, vol. 23, no. 2, 2002, pp. 253-8.
Sterbová H, Sevcíková P, Kvasnicková L, et al. Determination of lignans in Schisandra chinensis using micellar electrokinetic capillary chromatography. Electrophoresis. 2002;23(2):253-8.
Sterbová, H., Sevcíková, P., Kvasnicková, L., Glatz, Z., & Slanina, J. (2002). Determination of lignans in Schisandra chinensis using micellar electrokinetic capillary chromatography. Electrophoresis, 23(2), 253-8.
Sterbová H, et al. Determination of Lignans in Schisandra Chinensis Using Micellar Electrokinetic Capillary Chromatography. Electrophoresis. 2002;23(2):253-8. PubMed PMID: 11840532.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Determination of lignans in Schisandra chinensis using micellar electrokinetic capillary chromatography. AU - Sterbová,Hana, AU - Sevcíková,Petra, AU - Kvasnicková,Leona, AU - Glatz,Zdenek, AU - Slanina,Jirí, PY - 2002/2/13/pubmed PY - 2002/5/23/medline PY - 2002/2/13/entrez SP - 253 EP - 8 JF - Electrophoresis JO - Electrophoresis VL - 23 IS - 2 N2 - Micellar electrokinetic capillary chromatography (MEKC) has been developed as a promising method for the determination of lignans in plant samples. The separation conditions have been optimized with respect to the different parameters including sodium dodecyl sulfate (SDS) and acetonitrile concentration, pH of the background electrolyte, separation voltage, and capillary temperature. The background electrolyte consisting of 40 mM SDS and 35% acetonitrile in 10 mM tetraborate buffer (pH 9.3) was found to be the most suitable electrolyte for this analysis. The applied voltage of 28 kV (positive polarity) and the capillary temperature 25 degrees C gave the best separation of lignans. The interday reproducibility of the peak areas and the migration times was below 2.0%. The results of MEKC analyses were compared with those obtained by capillary electrochromatography (CEC) and reversed-phase high-performance liquid chromatography (RP-HPLC). The possibilities of using this method for the determination of lignans in drug and in serum samples were also tested. SN - 0173-0835 UR - https://www.unboundmedicine.com/medline/citation/11840532/Determination_of_lignans_in_Schisandra_chinensis_using_micellar_electrokinetic_capillary_chromatography_ L2 - https://doi.org/10.1002/1522-2683(200202)23:2<253::AID-ELPS253>3.0.CO;2-H DB - PRIME DP - Unbound Medicine ER -