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An immunocytochemical profile of the endocrine pancreas using an occlusive duct ligation model.
JOP. 2000 Nov; 1(4):191-203.JOP

Abstract

CONTEXT

Ligation of the pancreatic duct, distally to its confluence into the bile duct has been shown to induce endocrine tissue regeneration. The surplus endocrine tissue formed is presumed to be able to replace pathologically and/or experimentally compromised tissue.

OBJECTIVE

This is a quantitative study on the histology of duct ligated pancreas employing immunocytochemistry and computerised morphometry.

INTERVENTIONS

Pancreatic duct ligation was performed on 25 groups of six normal Sprague-Dawley rats. Experimental animals were sacrificed at 12-hour intervals from day one to ten post-duct ligation and every 24 hours thereafter to day 14, the pancreas removed, fixed and processed. Six consecutive 3-6 micron serial sections were cut on a rotary hand microtome, floated onto 3-aminopropyl-trimethoxysilan coated slides and alternatively immunocytochemically stained for insulin, glucagon, pancreatic polypeptide and somatostatin.

RESULTS

Pancreas transformation between days 1/2 and 3 1/2 was characterised by acinar deletion and the appearance of immunoreactive cells for the primary endocrine hormones. Transdifferentiation of existing endocrine tissue saw islet insulin core cells replaced by pancreatic polypeptide- and somatostatin positive cells, glucagon deletion and random appearance of all endocrine cell types within the inter-islet interstitium by day 3 1/2. Days 4 to 14 were characterised by cellular migration and islet reconstruction.

CONCLUSIONS

To date our laboratory has investigated transplantation of foetal tissue beneath the renal capsule in syngeneic, isogeneic and allogeneic normal and diabetic rats. As pancreatic duct ligation induces the development of surplus endocrine tissue our next step would be to investigate the use of ligated pancreas as a replacement for foetal tissue.

Authors+Show Affiliations

Department of Anatomy and Histology, Faculty of Medicine, University of Stellenbosch. Tygerberg, South Africa. bjp@gerga.sun.ac.zaNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Comparative Study
Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

11856861

Citation

Page, B J., et al. "An Immunocytochemical Profile of the Endocrine Pancreas Using an Occlusive Duct Ligation Model." JOP : Journal of the Pancreas, vol. 1, no. 4, 2000, pp. 191-203.
Page BJ, du Toit DF, Muller CJ, et al. An immunocytochemical profile of the endocrine pancreas using an occlusive duct ligation model. JOP. 2000;1(4):191-203.
Page, B. J., du Toit, D. F., Muller, C. J., Mattysen, J., & Lyners, R. (2000). An immunocytochemical profile of the endocrine pancreas using an occlusive duct ligation model. JOP : Journal of the Pancreas, 1(4), 191-203.
Page BJ, et al. An Immunocytochemical Profile of the Endocrine Pancreas Using an Occlusive Duct Ligation Model. JOP. 2000;1(4):191-203. PubMed PMID: 11856861.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - An immunocytochemical profile of the endocrine pancreas using an occlusive duct ligation model. AU - Page,B J, AU - du Toit,D F, AU - Muller,C J, AU - Mattysen,J, AU - Lyners,R, PY - 2002/2/22/pubmed PY - 2002/4/9/medline PY - 2002/2/22/entrez SP - 191 EP - 203 JF - JOP : Journal of the pancreas JO - JOP VL - 1 IS - 4 N2 - CONTEXT: Ligation of the pancreatic duct, distally to its confluence into the bile duct has been shown to induce endocrine tissue regeneration. The surplus endocrine tissue formed is presumed to be able to replace pathologically and/or experimentally compromised tissue. OBJECTIVE: This is a quantitative study on the histology of duct ligated pancreas employing immunocytochemistry and computerised morphometry. INTERVENTIONS: Pancreatic duct ligation was performed on 25 groups of six normal Sprague-Dawley rats. Experimental animals were sacrificed at 12-hour intervals from day one to ten post-duct ligation and every 24 hours thereafter to day 14, the pancreas removed, fixed and processed. Six consecutive 3-6 micron serial sections were cut on a rotary hand microtome, floated onto 3-aminopropyl-trimethoxysilan coated slides and alternatively immunocytochemically stained for insulin, glucagon, pancreatic polypeptide and somatostatin. RESULTS: Pancreas transformation between days 1/2 and 3 1/2 was characterised by acinar deletion and the appearance of immunoreactive cells for the primary endocrine hormones. Transdifferentiation of existing endocrine tissue saw islet insulin core cells replaced by pancreatic polypeptide- and somatostatin positive cells, glucagon deletion and random appearance of all endocrine cell types within the inter-islet interstitium by day 3 1/2. Days 4 to 14 were characterised by cellular migration and islet reconstruction. CONCLUSIONS: To date our laboratory has investigated transplantation of foetal tissue beneath the renal capsule in syngeneic, isogeneic and allogeneic normal and diabetic rats. As pancreatic duct ligation induces the development of surplus endocrine tissue our next step would be to investigate the use of ligated pancreas as a replacement for foetal tissue. SN - 1590-8577 UR - https://www.unboundmedicine.com/medline/citation/11856861/An_immunocytochemical_profile_of_the_endocrine_pancreas_using_an_occlusive_duct_ligation_model_ L2 - https://antibodies.cancer.gov/detail/CPTC-HLA-B-1 DB - PRIME DP - Unbound Medicine ER -