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Analysis of plant hormones in tobacco flowers by micellar electrokinetic capillary chromatography coupled with on-line large volume sample stacking.
J Chromatogr A. 2002 Feb 01; 945(1-2):257-65.JC

Abstract

Micellar electrokinetic capillary chromatography was developed to analyze plant hormones including gibberellic acid, abscisic acid, indole-3-acetic acid, alpha-naphthaleneacetic acid, 2,4-dichlorophenoxyacetic acid, kinetin-6-furfurylaminopurine and N6-benzyladenine. The influences of some crucial parameters including buffer concentration, pH value, micelle concentration and applied voltage on electrophoretic separation were investigated. Under optimum conditions (50 mM borate as the running buffer containing 50 mM sodium dodecylsulfate, pH 8.0; separation voltage: -15 kV; injection: hydrodynamic injection, 5 s at 50 mbar; temperature: 25 degrees C), a complete separation of seven plant hormones was accomplished within 30 min. Emphasis was placed on improving detection sensitivity in order to detect small amounts of hormones in plant tissue. Multiple wavelength detection and expanded bubble cell capillary were used with enrichment factors of 2 and 3, respectively. In addition, an on-line concentration method of large volume sample stacking was designed. Enrichment factors of up to approximately 10-600 were achieved for these hormones with detection limits down to 0.306 ng/ml. The method was successfully applied to analyzing abscisic acid in flowers of transgenic tobacco.

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Authors+Show Affiliations

Liu BF
Key Laboratory of MOE for Plant Developmental Biology, Center for Developmental Biology, College of Life Science, Wuhan University, PR China.
Zhong XH
No affiliation info available
Lu YT
No affiliation info available

MeSH

BuffersChromatography, Micellar Electrokinetic CapillaryPlant Growth RegulatorsSensitivity and SpecificityTobacco

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

11860141

Citation

Liu, Bi-Feng, et al. "Analysis of Plant Hormones in Tobacco Flowers By Micellar Electrokinetic Capillary Chromatography Coupled With On-line Large Volume Sample Stacking." Journal of Chromatography. A, vol. 945, no. 1-2, 2002, pp. 257-65.
Liu BF, Zhong XH, Lu YT. Analysis of plant hormones in tobacco flowers by micellar electrokinetic capillary chromatography coupled with on-line large volume sample stacking. J Chromatogr A. 2002;945(1-2):257-65.
Liu, B. F., Zhong, X. H., & Lu, Y. T. (2002). Analysis of plant hormones in tobacco flowers by micellar electrokinetic capillary chromatography coupled with on-line large volume sample stacking. Journal of Chromatography. A, 945(1-2), 257-65.
Liu BF, Zhong XH, Lu YT. Analysis of Plant Hormones in Tobacco Flowers By Micellar Electrokinetic Capillary Chromatography Coupled With On-line Large Volume Sample Stacking. J Chromatogr A. 2002 Feb 1;945(1-2):257-65. PubMed PMID: 11860141.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Analysis of plant hormones in tobacco flowers by micellar electrokinetic capillary chromatography coupled with on-line large volume sample stacking. AU - Liu,Bi-Feng, AU - Zhong,Xue-Hua, AU - Lu,Ying-Tang, PY - 2002/2/28/pubmed PY - 2002/8/1/medline PY - 2002/2/28/entrez SP - 257 EP - 65 JF - Journal of chromatography. A JO - J Chromatogr A VL - 945 IS - 1-2 N2 - Micellar electrokinetic capillary chromatography was developed to analyze plant hormones including gibberellic acid, abscisic acid, indole-3-acetic acid, alpha-naphthaleneacetic acid, 2,4-dichlorophenoxyacetic acid, kinetin-6-furfurylaminopurine and N6-benzyladenine. The influences of some crucial parameters including buffer concentration, pH value, micelle concentration and applied voltage on electrophoretic separation were investigated. Under optimum conditions (50 mM borate as the running buffer containing 50 mM sodium dodecylsulfate, pH 8.0; separation voltage: -15 kV; injection: hydrodynamic injection, 5 s at 50 mbar; temperature: 25 degrees C), a complete separation of seven plant hormones was accomplished within 30 min. Emphasis was placed on improving detection sensitivity in order to detect small amounts of hormones in plant tissue. Multiple wavelength detection and expanded bubble cell capillary were used with enrichment factors of 2 and 3, respectively. In addition, an on-line concentration method of large volume sample stacking was designed. Enrichment factors of up to approximately 10-600 were achieved for these hormones with detection limits down to 0.306 ng/ml. The method was successfully applied to analyzing abscisic acid in flowers of transgenic tobacco. SN - 0021-9673 UR - https://www.unboundmedicine.com/medline/citation/11860141/Analysis_of_plant_hormones_in_tobacco_flowers_by_micellar_electrokinetic_capillary_chromatography_coupled_with_on_line_large_volume_sample_stacking_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0021-9673(01)01503-5 DB - PRIME DP - Unbound Medicine ER -