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Changes in P2X receptor responses of sensory neurons from P2X3-deficient mice.
Eur J Neurosci 2001; 14(11):1784-92EJ

Abstract

Dorsal root ganglion (DRG) neurons respond to ATP with transient, persistent or biphasic inward currents. In contrast, the ATP responses in nodose neurons are persistent. These sustained currents are also heterogeneous, with one component being accounted for by P2X2/3 receptors, and the residual response probably mediated by P2X2 receptors, although the direct evidence for this has been lacking. In the present study, we examined the P2X receptors on DRG and nodose neurons from P2X3-deficient (P2X3-/-) mice, using whole cell voltage-clamp recording and immunohistochemistry. We found that all P2X3-/- DRG neurons lacked rapidly desensitizing response to ATP, and both DRG and nodose neurons from P2X3-null mutant mice no longer responded to alpha,beta-methylene ATP (alphabetameATP). In contrast, ATP evoked persistent inward current in 12% of DRG neurons and 84% of nodose neurons from P2X3-/- mice. This retained persistent response to ATP on nodose neurons had an EC50 for ATP of 77 microm, was antagonized by Cibacron blue and pyridoxal-5-phosphate-6-azophenyl-2',4'-disulphonic acid, potentiated by Zn2+ and acidification, but not enhanced by ivermectin or diinosine pentaphosphate. 2',3'-O-Trinitrophenyl-ATP antagonized this response with an IC50 of 8 microm. All these properties are consistent with those of recombinant P2X2 homomeric receptors. Furthermore, specific P2X2 receptor immunoreactivity detected in wild-type sensory neurons was unaltered in null mutant mice. Therefore, the alphabetameATP-insensitive persistent responses on nodose neurons are likely to be mediated by P2X2 homomers, which contribute to 60% of currents evoked by 100 microm ATP in the wild type.

Authors+Show Affiliations

Autonomic Neuroscience Institute, Department of Anatomy and Developmental Biology, Royal Free and University College Medical School, Rowland Hill Street, London, NW3 2PF, UK. y.zhong@ucl.ac.ukNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

11860473

Citation

Zhong, Y, et al. "Changes in P2X Receptor Responses of Sensory Neurons From P2X3-deficient Mice." The European Journal of Neuroscience, vol. 14, no. 11, 2001, pp. 1784-92.
Zhong Y, Dunn PM, Bardini M, et al. Changes in P2X receptor responses of sensory neurons from P2X3-deficient mice. Eur J Neurosci. 2001;14(11):1784-92.
Zhong, Y., Dunn, P. M., Bardini, M., Ford, A. P., Cockayne, D. A., & Burnstock, G. (2001). Changes in P2X receptor responses of sensory neurons from P2X3-deficient mice. The European Journal of Neuroscience, 14(11), pp. 1784-92.
Zhong Y, et al. Changes in P2X Receptor Responses of Sensory Neurons From P2X3-deficient Mice. Eur J Neurosci. 2001;14(11):1784-92. PubMed PMID: 11860473.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Changes in P2X receptor responses of sensory neurons from P2X3-deficient mice. AU - Zhong,Y, AU - Dunn,P M, AU - Bardini,M, AU - Ford,A P, AU - Cockayne,D A, AU - Burnstock,G, PY - 2002/2/28/pubmed PY - 2002/4/6/medline PY - 2002/2/28/entrez SP - 1784 EP - 92 JF - The European journal of neuroscience JO - Eur. J. Neurosci. VL - 14 IS - 11 N2 - Dorsal root ganglion (DRG) neurons respond to ATP with transient, persistent or biphasic inward currents. In contrast, the ATP responses in nodose neurons are persistent. These sustained currents are also heterogeneous, with one component being accounted for by P2X2/3 receptors, and the residual response probably mediated by P2X2 receptors, although the direct evidence for this has been lacking. In the present study, we examined the P2X receptors on DRG and nodose neurons from P2X3-deficient (P2X3-/-) mice, using whole cell voltage-clamp recording and immunohistochemistry. We found that all P2X3-/- DRG neurons lacked rapidly desensitizing response to ATP, and both DRG and nodose neurons from P2X3-null mutant mice no longer responded to alpha,beta-methylene ATP (alphabetameATP). In contrast, ATP evoked persistent inward current in 12% of DRG neurons and 84% of nodose neurons from P2X3-/- mice. This retained persistent response to ATP on nodose neurons had an EC50 for ATP of 77 microm, was antagonized by Cibacron blue and pyridoxal-5-phosphate-6-azophenyl-2',4'-disulphonic acid, potentiated by Zn2+ and acidification, but not enhanced by ivermectin or diinosine pentaphosphate. 2',3'-O-Trinitrophenyl-ATP antagonized this response with an IC50 of 8 microm. All these properties are consistent with those of recombinant P2X2 homomeric receptors. Furthermore, specific P2X2 receptor immunoreactivity detected in wild-type sensory neurons was unaltered in null mutant mice. Therefore, the alphabetameATP-insensitive persistent responses on nodose neurons are likely to be mediated by P2X2 homomers, which contribute to 60% of currents evoked by 100 microm ATP in the wild type. SN - 0953-816X UR - https://www.unboundmedicine.com/medline/citation/11860473/Changes_in_P2X_receptor_responses_of_sensory_neurons_from_P2X3_deficient_mice_ L2 - https://onlinelibrary.wiley.com/resolve/openurl?genre=article&sid=nlm:pubmed&issn=0953-816X&date=2001&volume=14&issue=11&spage=1784 DB - PRIME DP - Unbound Medicine ER -