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Isolation and characterization of repetitive DNA sequences from Panax ginseng.
Mol Genet Genomics 2002; 266(6):951-61MG

Abstract

Repetitive sequences constitute a significant component of most eukaryotic genomes, and the isolation and characterization of repetitive DNA sequences provide an insight into the organization and evolution of the genome of interest. We report the isolation and characterization of the major classes of repetitive sequences from the genome of Panax ginseng. The isolation of repetitive DNA from P. ginseng was achieved by the reannealing of chemically hydrolyzed (200 bp-1 kb fragments) and heat-denatured genomic DNA to low C(o)t value. The low C(o)t fraction was cloned, and fifty-five P. ginseng clones were identified that contained repetitive sequences. Sequence analysis revealed that the fraction includes repetitive telomeric sequences, species-specific satellite sequences, chloroplast DNA fragments and sequences that are homologous to retrotransposons. Two of the retrotransposon-like sequences are homologous to Ty1/ copia-type retroelements of Zea mays, and six cloned sequences are homologous to various regions of the del retrotransposon of Lilium henryi. The del retrotransposon-like sequences and several novel repetitive DNA sequences from P. ginseng were used to differentiate P. ginseng from P. quinquefolius, and should be useful for evolutionary studies of these disjunct species.

Authors+Show Affiliations

Department of Zoology, University of Hong Kong, Pokfulam, Hong Kong.No affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

11862489

Citation

Ho, I S H., and F C. Leung. "Isolation and Characterization of Repetitive DNA Sequences From Panax Ginseng." Molecular Genetics and Genomics : MGG, vol. 266, no. 6, 2002, pp. 951-61.
Ho IS, Leung FC. Isolation and characterization of repetitive DNA sequences from Panax ginseng. Mol Genet Genomics. 2002;266(6):951-61.
Ho, I. S., & Leung, F. C. (2002). Isolation and characterization of repetitive DNA sequences from Panax ginseng. Molecular Genetics and Genomics : MGG, 266(6), pp. 951-61.
Ho IS, Leung FC. Isolation and Characterization of Repetitive DNA Sequences From Panax Ginseng. Mol Genet Genomics. 2002;266(6):951-61. PubMed PMID: 11862489.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Isolation and characterization of repetitive DNA sequences from Panax ginseng. AU - Ho,I S H, AU - Leung,F C, Y1 - 2001/12/19/ PY - 2001/08/18/received PY - 2001/10/30/accepted PY - 2002/2/28/pubmed PY - 2002/5/9/medline PY - 2002/2/28/entrez SP - 951 EP - 61 JF - Molecular genetics and genomics : MGG JO - Mol. Genet. Genomics VL - 266 IS - 6 N2 - Repetitive sequences constitute a significant component of most eukaryotic genomes, and the isolation and characterization of repetitive DNA sequences provide an insight into the organization and evolution of the genome of interest. We report the isolation and characterization of the major classes of repetitive sequences from the genome of Panax ginseng. The isolation of repetitive DNA from P. ginseng was achieved by the reannealing of chemically hydrolyzed (200 bp-1 kb fragments) and heat-denatured genomic DNA to low C(o)t value. The low C(o)t fraction was cloned, and fifty-five P. ginseng clones were identified that contained repetitive sequences. Sequence analysis revealed that the fraction includes repetitive telomeric sequences, species-specific satellite sequences, chloroplast DNA fragments and sequences that are homologous to retrotransposons. Two of the retrotransposon-like sequences are homologous to Ty1/ copia-type retroelements of Zea mays, and six cloned sequences are homologous to various regions of the del retrotransposon of Lilium henryi. The del retrotransposon-like sequences and several novel repetitive DNA sequences from P. ginseng were used to differentiate P. ginseng from P. quinquefolius, and should be useful for evolutionary studies of these disjunct species. SN - 1617-4615 UR - https://www.unboundmedicine.com/medline/citation/11862489/Isolation_and_characterization_of_repetitive_DNA_sequences_from_Panax_ginseng_ L2 - https://dx.doi.org/10.1007/s00438-001-0617-6 DB - PRIME DP - Unbound Medicine ER -