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Characterization of transient outward K+ current and ultra-rapid delayed rectifier K+ current in isolated human atrial myocytes from patients with congestive heart failure.
Acta Pharmacol Sin. 2002 Feb; 23(2):110-6.AP

Abstract

AIM

To study the properties of transient outward K+ current (Ito) and ultra-rapid delayed rectifier K+ current (IKur) in isolated human atrial myocytes from patients with congestive heart failure (CHF).

METHODS

Single cells were isolated from CHF patients with collagenase and protease. Ito and IKur were recorded using whole cell patch-clamp technique.

RESULTS

The activation and inactivation of I(to) were voltage-dependent and time-dependent. The half-activation and half-inactivation voltage were (15 +/- 12) mV and (-45 +/- 4) mV respectively. When membrane potential went up from -40 mV to +60 mV, the activation time constant means decreased from (6.9 +/- 2.3) ms to (1.40 +/- 0.20) ms, while the inactivation time constant means decreased from (69 +/- 17) ms to (21 +/- 14) ms. Otherwise, the mean reactivation time constants was (125 +/- 65) ms when the membrane potential was held at -80 mV, but the recovery was not complete during the interval observed. Ito showed less frequency-dependent reduction at test frequency between 0.2-2 Hz. Compared with Ito, the activation of IKur only showed voltage-dependence, without time-dependence. Its mean current densities was (3.4 +/- 0.7) pA/pF when test potential was +60 mV. The half activation voltage of IKur was (23 +/- 14) mV. No clear frequency-dependence was observed at the same frequency range of Ito either.

CONCLUSION

I(to) and IKur are important outward potassium channel currents in isolated human atrial myocytes from CHF patients and they have different kinetic properties.

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Authors+Show Affiliations

Xu ZX
Department of Pharmacology, Tongji Medical College of Huazhong University of Science and Technology, Wuhan 430030, China. xuzhengxin@yahoo.com
Jin MW
No affiliation info available

MeSH

AdolescentAdultCell SeparationDelayed Rectifier Potassium ChannelsHeart AtriaHeart FailureHumansMembrane PotentialsMiddle AgedMuscle CellsPatch-Clamp TechniquesPotassium ChannelsPotassium Channels, Voltage-Gated

Pub Type(s)

Journal Article

Language

eng

PubMed ID

11866869

Citation

Xu, Zheng-Xin, and Man-Wen Jin. "Characterization of Transient Outward K+ Current and Ultra-rapid Delayed Rectifier K+ Current in Isolated Human Atrial Myocytes From Patients With Congestive Heart Failure." Acta Pharmacologica Sinica, vol. 23, no. 2, 2002, pp. 110-6.
Xu ZX, Jin MW. Characterization of transient outward K+ current and ultra-rapid delayed rectifier K+ current in isolated human atrial myocytes from patients with congestive heart failure. Acta Pharmacol Sin. 2002;23(2):110-6.
Xu, Z. X., & Jin, M. W. (2002). Characterization of transient outward K+ current and ultra-rapid delayed rectifier K+ current in isolated human atrial myocytes from patients with congestive heart failure. Acta Pharmacologica Sinica, 23(2), 110-6.
Xu ZX, Jin MW. Characterization of Transient Outward K+ Current and Ultra-rapid Delayed Rectifier K+ Current in Isolated Human Atrial Myocytes From Patients With Congestive Heart Failure. Acta Pharmacol Sin. 2002;23(2):110-6. PubMed PMID: 11866869.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Characterization of transient outward K+ current and ultra-rapid delayed rectifier K+ current in isolated human atrial myocytes from patients with congestive heart failure. AU - Xu,Zheng-Xin, AU - Jin,Man-Wen, PY - 2002/2/28/pubmed PY - 2003/9/17/medline PY - 2002/2/28/entrez SP - 110 EP - 6 JF - Acta pharmacologica Sinica JO - Acta Pharmacol Sin VL - 23 IS - 2 N2 - AIM: To study the properties of transient outward K+ current (Ito) and ultra-rapid delayed rectifier K+ current (IKur) in isolated human atrial myocytes from patients with congestive heart failure (CHF). METHODS: Single cells were isolated from CHF patients with collagenase and protease. Ito and IKur were recorded using whole cell patch-clamp technique. RESULTS: The activation and inactivation of I(to) were voltage-dependent and time-dependent. The half-activation and half-inactivation voltage were (15 +/- 12) mV and (-45 +/- 4) mV respectively. When membrane potential went up from -40 mV to +60 mV, the activation time constant means decreased from (6.9 +/- 2.3) ms to (1.40 +/- 0.20) ms, while the inactivation time constant means decreased from (69 +/- 17) ms to (21 +/- 14) ms. Otherwise, the mean reactivation time constants was (125 +/- 65) ms when the membrane potential was held at -80 mV, but the recovery was not complete during the interval observed. Ito showed less frequency-dependent reduction at test frequency between 0.2-2 Hz. Compared with Ito, the activation of IKur only showed voltage-dependence, without time-dependence. Its mean current densities was (3.4 +/- 0.7) pA/pF when test potential was +60 mV. The half activation voltage of IKur was (23 +/- 14) mV. No clear frequency-dependence was observed at the same frequency range of Ito either. CONCLUSION: I(to) and IKur are important outward potassium channel currents in isolated human atrial myocytes from CHF patients and they have different kinetic properties. SN - 1671-4083 UR - https://www.unboundmedicine.com/medline/citation/11866869/Characterization_of_transient_outward_K+_current_and_ultra_rapid_delayed_rectifier_K+_current_in_isolated_human_atrial_myocytes_from_patients_with_congestive_heart_failure_ L2 - http://www.chinaphar.com/1671-4083/23/110.pdf DB - PRIME DP - Unbound Medicine ER -