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The mRNA export factor Dbp5 is associated with Balbiani ring mRNP from gene to cytoplasm.
EMBO J. 2002 Mar 01; 21(5):1177-87.EJ

Abstract

The DEAD box RNA helicase Dbp5 is essential for nucleocytoplasmic transport of mRNA-protein (mRNP) complexes. Dbp5 is present mainly in the cytoplasm and is enriched at the cytoplasmic side of nuclear pore complexes (NPCs), suggesting that it acts in the late part of mRNP export. Here, we visualize the assembly and transport of a specific mRNP particle, the Balbiani ring mRNP in the dipteran Chironomus tentans, and show that a Dbp5 homologue in C.tentans, Ct-Dbp5, binds to pre-mRNP co-transcriptionally and accompanies the mRNP to and through the nuclear pores and into the cytoplasm. We also demonstrate that Ct-Dbp5 accumulates in the nucleus and partly disappears from the NPC when nuclear export of mRNA is inhibited. The fact that Ct-Dbp5 is present along the exiting mRNP fibril extending from the nuclear pore into the cytoplasm supports the view that Ct-Dbp5 is involved in restructuring the mRNP prior to translation. Finally, the addition of the export factor Dbp5 to the growing transcript highlights the importance of the co-transcriptional loading process in determining the fate of mRNA.

Authors+Show Affiliations

Department of Cell and Molecular Biology, Medical Nobel Institute, Karolinska Institutet, SE-17177 Stockholm, Sweden.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Comparative Study
Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

11867546

Citation

Zhao, Jian, et al. "The mRNA Export Factor Dbp5 Is Associated With Balbiani Ring mRNP From Gene to Cytoplasm." The EMBO Journal, vol. 21, no. 5, 2002, pp. 1177-87.
Zhao J, Jin SB, Björkroth B, et al. The mRNA export factor Dbp5 is associated with Balbiani ring mRNP from gene to cytoplasm. EMBO J. 2002;21(5):1177-87.
Zhao, J., Jin, S. B., Björkroth, B., Wieslander, L., & Daneholt, B. (2002). The mRNA export factor Dbp5 is associated with Balbiani ring mRNP from gene to cytoplasm. The EMBO Journal, 21(5), 1177-87.
Zhao J, et al. The mRNA Export Factor Dbp5 Is Associated With Balbiani Ring mRNP From Gene to Cytoplasm. EMBO J. 2002 Mar 1;21(5):1177-87. PubMed PMID: 11867546.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - The mRNA export factor Dbp5 is associated with Balbiani ring mRNP from gene to cytoplasm. AU - Zhao,Jian, AU - Jin,Shao-Bo, AU - Björkroth,Birgitta, AU - Wieslander,Lars, AU - Daneholt,Bertil, PY - 2002/2/28/pubmed PY - 2002/5/15/medline PY - 2002/2/28/entrez SP - 1177 EP - 87 JF - The EMBO journal JO - EMBO J VL - 21 IS - 5 N2 - The DEAD box RNA helicase Dbp5 is essential for nucleocytoplasmic transport of mRNA-protein (mRNP) complexes. Dbp5 is present mainly in the cytoplasm and is enriched at the cytoplasmic side of nuclear pore complexes (NPCs), suggesting that it acts in the late part of mRNP export. Here, we visualize the assembly and transport of a specific mRNP particle, the Balbiani ring mRNP in the dipteran Chironomus tentans, and show that a Dbp5 homologue in C.tentans, Ct-Dbp5, binds to pre-mRNP co-transcriptionally and accompanies the mRNP to and through the nuclear pores and into the cytoplasm. We also demonstrate that Ct-Dbp5 accumulates in the nucleus and partly disappears from the NPC when nuclear export of mRNA is inhibited. The fact that Ct-Dbp5 is present along the exiting mRNP fibril extending from the nuclear pore into the cytoplasm supports the view that Ct-Dbp5 is involved in restructuring the mRNP prior to translation. Finally, the addition of the export factor Dbp5 to the growing transcript highlights the importance of the co-transcriptional loading process in determining the fate of mRNA. SN - 0261-4189 UR - https://www.unboundmedicine.com/medline/citation/11867546/The_mRNA_export_factor_Dbp5_is_associated_with_Balbiani_ring_mRNP_from_gene_to_cytoplasm_ L2 - https://doi.org/10.1093/emboj/21.5.1177 DB - PRIME DP - Unbound Medicine ER -