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Expression and function of 4-1BB and 4-1BB ligand on murine dendritic cells.
Int Immunol. 2002 Mar; 14(3):275-86.II

Abstract

4-1BB (CDw137) and its ligand (4-1BBL) have been implicated in cellular immune responses. To further characterize the expression and function of 4-1BBL, we newly generated an anti-mouse 4-1BBL mAb (TKS-1), which can inhibit the interaction of 4-1BBL with 4-1BB. Flow cytometric analyses using TKS-1 and an anti-mouse 4-1BB mAb indicated that 4-1BB was inducible on both CD4(+) and CD8(+) splenic T cells by stimulation with immobilized anti-CD3 mAb, but 4-1BBL was not expressed on resting or activated T cells. 4-1BBL expression was inducible on splenic B cells by stimulation with anti-IgM antibody plus anti-CD40 mAb, on peritoneal macrophages by stimulation with lipopolysaccharide (LPS) and on splenic dendritic cells (DC) by stimulation with anti-CD40 mAb or LPS. Interestingly, splenic DC expressed 4-1BB constitutively, which was down-regulated by anti-CD40 stimulation. Co-culture of splenic DC with 4-1BBL-transfected cells or 4-1BBL-expressing tumor cell lines led to cytokine (IL-6 and IL-12) production and co-stimulatory molecule up-regulation by splenic DC, indicating that 4-1BBL can directly activate DC. Moreover, IL-12 production by anti-CD40-stimulated DC was partially inhibited by TKS-1. These results suggest that 4-1BB expressed on DC may be involved in DC activation through DC--tumor interaction and DC--DC interaction.

Authors+Show Affiliations

Department of Immunology, Juntendo University School of Medicine, 2-1-1 Hongo, Bunkyo-ku, Tokyo 113-8421, Japan.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

11867564

Citation

Futagawa, Toshiro, et al. "Expression and Function of 4-1BB and 4-1BB Ligand On Murine Dendritic Cells." International Immunology, vol. 14, no. 3, 2002, pp. 275-86.
Futagawa T, Akiba H, Kodama T, et al. Expression and function of 4-1BB and 4-1BB ligand on murine dendritic cells. Int Immunol. 2002;14(3):275-86.
Futagawa, T., Akiba, H., Kodama, T., Takeda, K., Hosoda, Y., Yagita, H., & Okumura, K. (2002). Expression and function of 4-1BB and 4-1BB ligand on murine dendritic cells. International Immunology, 14(3), 275-86.
Futagawa T, et al. Expression and Function of 4-1BB and 4-1BB Ligand On Murine Dendritic Cells. Int Immunol. 2002;14(3):275-86. PubMed PMID: 11867564.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Expression and function of 4-1BB and 4-1BB ligand on murine dendritic cells. AU - Futagawa,Toshiro, AU - Akiba,Hisaya, AU - Kodama,Tomohiro, AU - Takeda,Kazuyoshi, AU - Hosoda,Yasuyuki, AU - Yagita,Hideo, AU - Okumura,Ko, PY - 2002/2/28/pubmed PY - 2002/8/6/medline PY - 2002/2/28/entrez SP - 275 EP - 86 JF - International immunology JO - Int. Immunol. VL - 14 IS - 3 N2 - 4-1BB (CDw137) and its ligand (4-1BBL) have been implicated in cellular immune responses. To further characterize the expression and function of 4-1BBL, we newly generated an anti-mouse 4-1BBL mAb (TKS-1), which can inhibit the interaction of 4-1BBL with 4-1BB. Flow cytometric analyses using TKS-1 and an anti-mouse 4-1BB mAb indicated that 4-1BB was inducible on both CD4(+) and CD8(+) splenic T cells by stimulation with immobilized anti-CD3 mAb, but 4-1BBL was not expressed on resting or activated T cells. 4-1BBL expression was inducible on splenic B cells by stimulation with anti-IgM antibody plus anti-CD40 mAb, on peritoneal macrophages by stimulation with lipopolysaccharide (LPS) and on splenic dendritic cells (DC) by stimulation with anti-CD40 mAb or LPS. Interestingly, splenic DC expressed 4-1BB constitutively, which was down-regulated by anti-CD40 stimulation. Co-culture of splenic DC with 4-1BBL-transfected cells or 4-1BBL-expressing tumor cell lines led to cytokine (IL-6 and IL-12) production and co-stimulatory molecule up-regulation by splenic DC, indicating that 4-1BBL can directly activate DC. Moreover, IL-12 production by anti-CD40-stimulated DC was partially inhibited by TKS-1. These results suggest that 4-1BB expressed on DC may be involved in DC activation through DC--tumor interaction and DC--DC interaction. SN - 0953-8178 UR - https://www.unboundmedicine.com/medline/citation/11867564/Expression_and_function_of_4_1BB_and_4_1BB_ligand_on_murine_dendritic_cells_ L2 - https://academic.oup.com/intimm/article-lookup/doi/10.1093/intimm/14.3.275 DB - PRIME DP - Unbound Medicine ER -