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Long-term effects of light damage on the retina of albino and pigmented rats.
Invest Ophthalmol Vis Sci 2002; 43(3):813-20IO

Abstract

PURPOSE

To observe the morphology and physiology of the retina in rats 11 weeks after a constant (24-hour) but moderate (500-lux) illumination for 1 week.

METHODS

Levels of aspartate, gamma-aminobutyric acid (GABA), glutamate, glutamine, and taurine were measured by high-pressure liquid chromatography (HPLC) in the retina and vitreous humor of albino (Wistar) and pigmented (Long-Evans) rats. Semithin sections were used to determine retinal morphology. The TUNEL method was used to detect cells degenerating by apoptosis. Because the GABAergic system has been shown to be particularly sensitive to the loss of photoreceptors, an additional immunohistochemical study using anti-GABA, anti-glutamate decarboxylase (GAD)(67) and anti-GAD(65) antibodies was performed.

RESULTS

No apparent morphologic changes were found in the retina of pigmented rats after constant illumination, whereas in albino rats disappearance of photoreceptors (except in the extreme retinal periphery) and cell bodies was observed. A significant number of TUNEL-positive nuclei also occurred in the remaining nuclear and ganglion cell layers. However, no change in the distribution of GABA, GAD(67), and GAD(65) immunoreactivities was found in either strain under constant illumination compared with control animals. Constant illumination affected the retinal levels of aspartate, glutamate, glutamine, glycine in both strains, whereas GABA contents did not change and taurine was decreased only in albino rats. A significant increase of vitreal glutamate levels was also found in both strains and of taurine levels only in albino rats.

CONCLUSIONS

Phototoxicity can provoke durable retinal alterations beyond the period of lighting, suggesting progressive and probably continuous modifications of retinal physiology, even in pigmented animals in which the retina seems morphologically normal.

Authors+Show Affiliations

L'Institut National de la Santé et de la Recherche Médicale U 450, Laboratoire de Neuro-Cytologie Oculaire, 15 rue de l'Ecole de Médecine, 75006 Paris, France.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Comparative Study
Journal Article

Language

eng

PubMed ID

11867603

Citation

Wasowicz, Marguerite, et al. "Long-term Effects of Light Damage On the Retina of Albino and Pigmented Rats." Investigative Ophthalmology & Visual Science, vol. 43, no. 3, 2002, pp. 813-20.
Wasowicz M, Morice C, Ferrari P, et al. Long-term effects of light damage on the retina of albino and pigmented rats. Invest Ophthalmol Vis Sci. 2002;43(3):813-20.
Wasowicz, M., Morice, C., Ferrari, P., Callebert, J., & Versaux-Botteri, C. (2002). Long-term effects of light damage on the retina of albino and pigmented rats. Investigative Ophthalmology & Visual Science, 43(3), pp. 813-20.
Wasowicz M, et al. Long-term Effects of Light Damage On the Retina of Albino and Pigmented Rats. Invest Ophthalmol Vis Sci. 2002;43(3):813-20. PubMed PMID: 11867603.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Long-term effects of light damage on the retina of albino and pigmented rats. AU - Wasowicz,Marguerite, AU - Morice,Cécile, AU - Ferrari,Patricia, AU - Callebert,Jacques, AU - Versaux-Botteri,Claudine, PY - 2002/2/28/pubmed PY - 2002/3/29/medline PY - 2002/2/28/entrez SP - 813 EP - 20 JF - Investigative ophthalmology & visual science JO - Invest. Ophthalmol. Vis. Sci. VL - 43 IS - 3 N2 - PURPOSE: To observe the morphology and physiology of the retina in rats 11 weeks after a constant (24-hour) but moderate (500-lux) illumination for 1 week. METHODS: Levels of aspartate, gamma-aminobutyric acid (GABA), glutamate, glutamine, and taurine were measured by high-pressure liquid chromatography (HPLC) in the retina and vitreous humor of albino (Wistar) and pigmented (Long-Evans) rats. Semithin sections were used to determine retinal morphology. The TUNEL method was used to detect cells degenerating by apoptosis. Because the GABAergic system has been shown to be particularly sensitive to the loss of photoreceptors, an additional immunohistochemical study using anti-GABA, anti-glutamate decarboxylase (GAD)(67) and anti-GAD(65) antibodies was performed. RESULTS: No apparent morphologic changes were found in the retina of pigmented rats after constant illumination, whereas in albino rats disappearance of photoreceptors (except in the extreme retinal periphery) and cell bodies was observed. A significant number of TUNEL-positive nuclei also occurred in the remaining nuclear and ganglion cell layers. However, no change in the distribution of GABA, GAD(67), and GAD(65) immunoreactivities was found in either strain under constant illumination compared with control animals. Constant illumination affected the retinal levels of aspartate, glutamate, glutamine, glycine in both strains, whereas GABA contents did not change and taurine was decreased only in albino rats. A significant increase of vitreal glutamate levels was also found in both strains and of taurine levels only in albino rats. CONCLUSIONS: Phototoxicity can provoke durable retinal alterations beyond the period of lighting, suggesting progressive and probably continuous modifications of retinal physiology, even in pigmented animals in which the retina seems morphologically normal. SN - 0146-0404 UR - https://www.unboundmedicine.com/medline/citation/11867603/Long_term_effects_of_light_damage_on_the_retina_of_albino_and_pigmented_rats_ L2 - http://iovs.arvojournals.org/article.aspx?volume=43&page=813 DB - PRIME DP - Unbound Medicine ER -