Tags

Type your tag names separated by a space and hit enter

Postweaning exposure to gossypol results in epididymis-specific effects throughout puberty and adulthood in rats.
J Androl. 2002 Mar-Apr; 23(2):220-8.JA

Abstract

Gossypol, a yellow pigment found in cottonseeds, well known for its antifertility properties in animals, has been used as a contraceptive by men. The aims of this work were to evaluate the effects of gossypol throughout sexual development of male rats and to provide additional data to clarify the target site or sites of this compound in the male reproductive system. Gossypol (15 mg/kg per day) was given to animals from weaning through prepuberty (41 days), early puberty (51 days), puberty (61 days), and sexual maturity (91 days). Ventral prostate weight and fructose levels were similar in control and treated rats, suggesting that androgen levels were normal. No histological effects on the testis were detected, but there was a significant decrease in the sperm concentration in the cauda epididymidis of gossypol-treated animals killed at 61 and 91 days, as well as a significant increase in abnormal sperm in the vas deferens of treated animals. Moreover, the histology of the cauda epididymidis of the rats treated throughout puberty (ie, until days 51 and 61) showed a great number of round bodies in the lumen of the epididymis. These structures stained for the epididymis-specific protein E. Collectively, the data demonstrate that the epididymis is a target of gossypol when postweaning exposure extends throughout pubertal development, and that whereas more subtle histological effects commence around puberty, indicators reproductive competence are compromised in adulthood.

Authors+Show Affiliations

Department of Anatomy, Institute of Biosciences, State University of São Paulo, Botucatu, Brazil.No affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't
Research Support, U.S. Gov't, Non-P.H.S.

Language

eng

PubMed ID

11868815

Citation

Romualdo, Genair Silva, et al. "Postweaning Exposure to Gossypol Results in Epididymis-specific Effects Throughout Puberty and Adulthood in Rats." Journal of Andrology, vol. 23, no. 2, 2002, pp. 220-8.
Romualdo GS, Klinefelter GR, de K. Postweaning exposure to gossypol results in epididymis-specific effects throughout puberty and adulthood in rats. J Androl. 2002;23(2):220-8.
Romualdo, G. S., Klinefelter, G. R., & de, K. (2002). Postweaning exposure to gossypol results in epididymis-specific effects throughout puberty and adulthood in rats. Journal of Andrology, 23(2), 220-8.
Romualdo GS, Klinefelter GR, de K. Postweaning Exposure to Gossypol Results in Epididymis-specific Effects Throughout Puberty and Adulthood in Rats. J Androl. 2002 Mar-Apr;23(2):220-8. PubMed PMID: 11868815.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Postweaning exposure to gossypol results in epididymis-specific effects throughout puberty and adulthood in rats. AU - Romualdo,Genair Silva, AU - Klinefelter,Gary R, AU - de,KempinasWilmaGrava, PY - 2002/3/1/pubmed PY - 2002/8/23/medline PY - 2002/3/1/entrez SP - 220 EP - 8 JF - Journal of andrology JO - J Androl VL - 23 IS - 2 N2 - Gossypol, a yellow pigment found in cottonseeds, well known for its antifertility properties in animals, has been used as a contraceptive by men. The aims of this work were to evaluate the effects of gossypol throughout sexual development of male rats and to provide additional data to clarify the target site or sites of this compound in the male reproductive system. Gossypol (15 mg/kg per day) was given to animals from weaning through prepuberty (41 days), early puberty (51 days), puberty (61 days), and sexual maturity (91 days). Ventral prostate weight and fructose levels were similar in control and treated rats, suggesting that androgen levels were normal. No histological effects on the testis were detected, but there was a significant decrease in the sperm concentration in the cauda epididymidis of gossypol-treated animals killed at 61 and 91 days, as well as a significant increase in abnormal sperm in the vas deferens of treated animals. Moreover, the histology of the cauda epididymidis of the rats treated throughout puberty (ie, until days 51 and 61) showed a great number of round bodies in the lumen of the epididymis. These structures stained for the epididymis-specific protein E. Collectively, the data demonstrate that the epididymis is a target of gossypol when postweaning exposure extends throughout pubertal development, and that whereas more subtle histological effects commence around puberty, indicators reproductive competence are compromised in adulthood. SN - 0196-3635 UR - https://www.unboundmedicine.com/medline/citation/11868815/Postweaning_exposure_to_gossypol_results_in_epididymis_specific_effects_throughout_puberty_and_adulthood_in_rats_ L2 - https://onlinelibrary.wiley.com/resolve/openurl?genre=article&sid=nlm:pubmed&issn=0196-3635&date=2002&volume=23&issue=2&spage=220 DB - PRIME DP - Unbound Medicine ER -