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Characterization of the ves gene, which is expressed at a low temperature in Escherichia coli.
J Mol Microbiol Biotechnol 2002; 4(2):163-9JM

Abstract

A gene, designated ves, that is expressionally responsive to temperature was found in Escherichia coli. Experiments with a single-copy lacZ operon fusion and primer extension analysis revealed that ves was expressed at a low temperature with a peak around 25 degrees C but was hardly expressed at 42 degrees C. After a temperature downshift, the mRNA level increased until 6 to 12 h and then decreased. Consistently, an A + T-rich sequence similar to UP elements seen in cold-shock inducible cold-shock protein (Csp) genes was found up-stream of the ves promoter, and its 5'-untranslated region was found to share similarity with those of the cold-shock inducible and cold-adaptive cspA and cspB genes. Additionally, a putative down-stream box, which also exists in cold-inducible proteins, was found. The ves product was identified by overproduction and determination of its N-terminal sequence. Similarity of the C-terminal portion of Ves to the CspA family suggests that Ves belongs to this family. The results of gene-disruption experiments suggest that ves is not essential for E. coli.

Authors+Show Affiliations

Yamada M
Department of Biological Chemistry, Faculty of Agriculture, Yamaguchi University, Japan. yamada@agr.yamaguchi-u.ac.jp
Nagamitsu H
No affiliation info available
Izu H
No affiliation info available
Nakamura K
No affiliation info available
Talukder AA
No affiliation info available

MeSH

Amino Acid SequenceBacterial ProteinsBase SequenceCarrier ProteinsCold TemperatureDNA-Directed RNA PolymerasesEscherichia coliEscherichia coli ProteinsGene DeletionGene Expression Regulation, BacterialGenes, BacterialHeat-Shock ProteinsMolecular Sequence DataOperator Regions, GeneticRNA, MessengerReverse Transcriptase Polymerase Chain ReactionSequence Analysis, DNATemperatureTranscription, GeneticViral Proteins

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

11873911

Citation

Yamada, Mamoru, et al. "Characterization of the Ves Gene, Which Is Expressed at a Low Temperature in Escherichia Coli." Journal of Molecular Microbiology and Biotechnology, vol. 4, no. 2, 2002, pp. 163-9.
Yamada M, Nagamitsu H, Izu H, et al. Characterization of the ves gene, which is expressed at a low temperature in Escherichia coli. J Mol Microbiol Biotechnol. 2002;4(2):163-9.
Yamada, M., Nagamitsu, H., Izu, H., Nakamura, K., & Talukder, A. A. (2002). Characterization of the ves gene, which is expressed at a low temperature in Escherichia coli. Journal of Molecular Microbiology and Biotechnology, 4(2), pp. 163-9.
Yamada M, et al. Characterization of the Ves Gene, Which Is Expressed at a Low Temperature in Escherichia Coli. J Mol Microbiol Biotechnol. 2002;4(2):163-9. PubMed PMID: 11873911.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Characterization of the ves gene, which is expressed at a low temperature in Escherichia coli. AU - Yamada,Mamoru, AU - Nagamitsu,Hiroshi, AU - Izu,Hanae, AU - Nakamura,Kazunori, AU - Talukder,Ali Azam, PY - 2002/3/5/pubmed PY - 2002/9/11/medline PY - 2002/3/5/entrez SP - 163 EP - 9 JF - Journal of molecular microbiology and biotechnology JO - J. Mol. Microbiol. Biotechnol. VL - 4 IS - 2 N2 - A gene, designated ves, that is expressionally responsive to temperature was found in Escherichia coli. Experiments with a single-copy lacZ operon fusion and primer extension analysis revealed that ves was expressed at a low temperature with a peak around 25 degrees C but was hardly expressed at 42 degrees C. After a temperature downshift, the mRNA level increased until 6 to 12 h and then decreased. Consistently, an A + T-rich sequence similar to UP elements seen in cold-shock inducible cold-shock protein (Csp) genes was found up-stream of the ves promoter, and its 5'-untranslated region was found to share similarity with those of the cold-shock inducible and cold-adaptive cspA and cspB genes. Additionally, a putative down-stream box, which also exists in cold-inducible proteins, was found. The ves product was identified by overproduction and determination of its N-terminal sequence. Similarity of the C-terminal portion of Ves to the CspA family suggests that Ves belongs to this family. The results of gene-disruption experiments suggest that ves is not essential for E. coli. SN - 1464-1801 UR - https://www.unboundmedicine.com/medline/citation/11873911/Characterization_of_the_ves_gene_which_is_expressed_at_a_low_temperature_in_Escherichia_coli_ DB - PRIME DP - Unbound Medicine ER -