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A non-alpha7 nicotinic acetylcholine receptor modulates excitatory input to hippocampal CA1 interneurons.
J Neurophysiol. 2002 Mar; 87(3):1651-4.JN

Abstract

The nicotinic acetylcholine receptor (nAChR), particularly the alpha7 subtype, has received profound attention for its role in modifying excitatory postsynaptic currents (EPSCs) in hippocampal pyramidal neurons as well as in neurons from other brain regions. Here, we tested the possibility that an nAChR could affect EPSCs in the interneurons of rat hippocampal slices. Using whole-cell patch-clamp technique on CA1 stratum radiatum interneurons and U-tube application of agents, we show that nicotinic agonists enhance EPSC frequency in interneurons. Among the agents tested, cytisine and mecamylamine were the most effective agonist and antagonist, respectively, suggesting a role for alpha3beta4-containing nAChRs in the modulation of interneuron EPSCs. Ligands selective for the alpha7 nAChR had very little or no effect on interneuron EPSCs. Low concentrations of nicotine also enhanced EPSC frequency, implicating the involvement of non-alpha7 nAChRs in controlling interneuron excitability in smokers. We conclude that nAChR-dependent EPSC modulation in the hippocampus is both subtype- and neuron-specific and that a non-alpha7 nAChR, presumably alpha3beta4, controls glutamate transmission to CA1 interneurons.

Authors+Show Affiliations

Department of Pharmacology and Experimental Therapeutics, University of Maryland School of Medicine, 655 W. Baltimore Street, Baltimore, MD 21201, USA.No affiliation info available

Pub Type(s)

Journal Article
Research Support, U.S. Gov't, P.H.S.

Language

eng

PubMed ID

11877536

Citation

Alkondon, Manickavasagom, and Edson X. Albuquerque. "A Non-alpha7 Nicotinic Acetylcholine Receptor Modulates Excitatory Input to Hippocampal CA1 Interneurons." Journal of Neurophysiology, vol. 87, no. 3, 2002, pp. 1651-4.
Alkondon M, Albuquerque EX. A non-alpha7 nicotinic acetylcholine receptor modulates excitatory input to hippocampal CA1 interneurons. J Neurophysiol. 2002;87(3):1651-4.
Alkondon, M., & Albuquerque, E. X. (2002). A non-alpha7 nicotinic acetylcholine receptor modulates excitatory input to hippocampal CA1 interneurons. Journal of Neurophysiology, 87(3), 1651-4.
Alkondon M, Albuquerque EX. A Non-alpha7 Nicotinic Acetylcholine Receptor Modulates Excitatory Input to Hippocampal CA1 Interneurons. J Neurophysiol. 2002;87(3):1651-4. PubMed PMID: 11877536.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - A non-alpha7 nicotinic acetylcholine receptor modulates excitatory input to hippocampal CA1 interneurons. AU - Alkondon,Manickavasagom, AU - Albuquerque,Edson X, PY - 2002/3/6/pubmed PY - 2002/5/22/medline PY - 2002/3/6/entrez SP - 1651 EP - 4 JF - Journal of neurophysiology JO - J. Neurophysiol. VL - 87 IS - 3 N2 - The nicotinic acetylcholine receptor (nAChR), particularly the alpha7 subtype, has received profound attention for its role in modifying excitatory postsynaptic currents (EPSCs) in hippocampal pyramidal neurons as well as in neurons from other brain regions. Here, we tested the possibility that an nAChR could affect EPSCs in the interneurons of rat hippocampal slices. Using whole-cell patch-clamp technique on CA1 stratum radiatum interneurons and U-tube application of agents, we show that nicotinic agonists enhance EPSC frequency in interneurons. Among the agents tested, cytisine and mecamylamine were the most effective agonist and antagonist, respectively, suggesting a role for alpha3beta4-containing nAChRs in the modulation of interneuron EPSCs. Ligands selective for the alpha7 nAChR had very little or no effect on interneuron EPSCs. Low concentrations of nicotine also enhanced EPSC frequency, implicating the involvement of non-alpha7 nAChRs in controlling interneuron excitability in smokers. We conclude that nAChR-dependent EPSC modulation in the hippocampus is both subtype- and neuron-specific and that a non-alpha7 nAChR, presumably alpha3beta4, controls glutamate transmission to CA1 interneurons. SN - 0022-3077 UR - https://www.unboundmedicine.com/medline/citation/11877536/A_non_alpha7_nicotinic_acetylcholine_receptor_modulates_excitatory_input_to_hippocampal_CA1_interneurons_ L2 - http://www.physiology.org/doi/full/10.1152/jn.00708.2001?url_ver=Z39.88-2003&rfr_id=ori:rid:crossref.org&rfr_dat=cr_pub=pubmed DB - PRIME DP - Unbound Medicine ER -