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Pharmacological properties of nicotinic acetylcholine receptors in isolated Locusta migratoria neurones.
Microsc Res Tech. 2002 Feb 15; 56(4):249-55.MR

Abstract

Mechanically dissociated neuronal cell bodies from the thoracic ganglia of Locusta migratoria were viable in culture conditions for up to 2 days and were voltage-clamped to record the effects of GABAergic drugs and physostigmine on the membrane conductance and ACh responses of the dissociated cells. Bicuculline, hydrastine, and gabazine inhibited the EC50 ACh responses of the cells. Both bicuculline and hydrastine were full inhibitors of the ACh responses but gabazine behaved as a partial inhibitor. Bicuculline, hydrastine, and gabazine inhibited the ACh responses in a non-competitive and voltage-independent fashion, suggesting that they are allosteric inhibitors of locust nicotinic ACh receptors. Physostigmine activated currents when applied onto isolated locust neurones. The responses activated by physostigmine were inhibited competitively by tubocurarine, which indicates that physostigmine interacts with the ACh site of locust nicotinic ACh receptors. However, maximal concentrations of physostigmine elicited currents of smaller amplitudes to those evoked by maximal ACh concentrations. Single-channel recordings suggest that the partial efficacy of physostigmine may reflect the low frequency of opening of physostigmine-induced single currents relative to that of ACh-single currents.

Authors+Show Affiliations

School of Biological and Molecular Sciences, Oxford Brookes University, Oxford OX3 0BP, United Kingdom.No affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article

Language

eng

PubMed ID

11877800

Citation

Jackson, Charles, et al. "Pharmacological Properties of Nicotinic Acetylcholine Receptors in Isolated Locusta Migratoria Neurones." Microscopy Research and Technique, vol. 56, no. 4, 2002, pp. 249-55.
Jackson C, Bermudez I, Beadle DJ. Pharmacological properties of nicotinic acetylcholine receptors in isolated Locusta migratoria neurones. Microsc Res Tech. 2002;56(4):249-55.
Jackson, C., Bermudez, I., & Beadle, D. J. (2002). Pharmacological properties of nicotinic acetylcholine receptors in isolated Locusta migratoria neurones. Microscopy Research and Technique, 56(4), 249-55.
Jackson C, Bermudez I, Beadle DJ. Pharmacological Properties of Nicotinic Acetylcholine Receptors in Isolated Locusta Migratoria Neurones. Microsc Res Tech. 2002 Feb 15;56(4):249-55. PubMed PMID: 11877800.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Pharmacological properties of nicotinic acetylcholine receptors in isolated Locusta migratoria neurones. AU - Jackson,Charles, AU - Bermudez,Isabel, AU - Beadle,David J, PY - 2002/3/6/pubmed PY - 2002/4/19/medline PY - 2002/3/6/entrez SP - 249 EP - 55 JF - Microscopy research and technique JO - Microsc Res Tech VL - 56 IS - 4 N2 - Mechanically dissociated neuronal cell bodies from the thoracic ganglia of Locusta migratoria were viable in culture conditions for up to 2 days and were voltage-clamped to record the effects of GABAergic drugs and physostigmine on the membrane conductance and ACh responses of the dissociated cells. Bicuculline, hydrastine, and gabazine inhibited the EC50 ACh responses of the cells. Both bicuculline and hydrastine were full inhibitors of the ACh responses but gabazine behaved as a partial inhibitor. Bicuculline, hydrastine, and gabazine inhibited the ACh responses in a non-competitive and voltage-independent fashion, suggesting that they are allosteric inhibitors of locust nicotinic ACh receptors. Physostigmine activated currents when applied onto isolated locust neurones. The responses activated by physostigmine were inhibited competitively by tubocurarine, which indicates that physostigmine interacts with the ACh site of locust nicotinic ACh receptors. However, maximal concentrations of physostigmine elicited currents of smaller amplitudes to those evoked by maximal ACh concentrations. Single-channel recordings suggest that the partial efficacy of physostigmine may reflect the low frequency of opening of physostigmine-induced single currents relative to that of ACh-single currents. SN - 1059-910X UR - https://www.unboundmedicine.com/medline/citation/11877800/Pharmacological_properties_of_nicotinic_acetylcholine_receptors_in_isolated_Locusta_migratoria_neurones_ L2 - https://doi.org/10.1002/jemt.10028 DB - PRIME DP - Unbound Medicine ER -